Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus
Objetive: Characterization of macrolide, lincosamide and streptogramin (MLS) resistance mechanisms in Streptococcus and Enterococcus in La Rioja, from 1996 to 2001. Distribution of erythromcyin resistance phenotypes and involved mechanisms were studied for both genera, as well as temporary evolutio...
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Universidad de La Rioja (España)
2002
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Objetive:
Characterization of macrolide, lincosamide and streptogramin (MLS) resistance mechanisms in Streptococcus and Enterococcus in La Rioja, from 1996 to 2001.
Distribution of erythromcyin resistance phenotypes and involved mechanisms were studied for both genera, as well as temporary evolution and clonal diversity for erythromycin-resistant Streptococcus isolates. A new gene that we called msrC was described for an enterococcal species.
Conclusions:
High erythromycin resistance rates were detected for beta-haemolytic group A, B, C and G Streptococcus isolates in La Rioja (13-25%) and it was even higher for S. pneumoniae isolates (54%). Percentages of erythromycin-resistant isolates decreased, from 35 to 20% and from 64 to 47%, for S. pyogenes and S. pneumoniae, respectively. M resistance phenotype was the most frequently found among erythromycin-resistant S. pyogenes isolates (80%). MLSB resistance phenotype was exclusive for beta-haemolytic Streptococcus group C and G, and it was >97% for S. agalactiae and S. pneumoniae. mef(A) or mef(E) genes, associated with M resistance phenotype, and erm(B) or erm(TR) genes, associated with MLSB resistance phenotype, were predominant in Streptococcus, depending on the species. Beta-haemolytic erythromycin-resistant Streptococcus isolates with the same clonal origin were detected in different years of the study.
The erm(B) gene was detected in all erythromycin highly-resistant Enterococcus isolates, with the exception of one isolate of E. faecium which presented the erm(A) gene. A new gene that we called msrC was detected in all E. faecium isolates, independently of their erythromycin resistance pattern. The remaining species of Enterococcus analyzed did not present msrC. The msrC gene, described in E. faecium, contains 1479 bp and it has 53% identity with msr(A) gene, described in Staphylococcus and related to macrolide and streptogramin B resistance in this bacteria. The msrC gene codifies a protein of 492 amino acids and it belongs to a family of efflux pumps genes. An erythromycin efflux system, dependent on energy, was seen in E. faecium isolates with different levels of resistance to this macrolide. The efflux pump MsrC could be related to this erythromycin efflux. |
| author2 |
Torres Manrique, Carmen (Universidad de La Rioja) |
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Torres Manrique, Carmen (Universidad de La Rioja) Portillo Barrio, Aránzazu |
| format |
text (thesis) |
| author |
Portillo Barrio, Aránzazu |
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Portillo Barrio, Aránzazu Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| author_sort |
Portillo Barrio, Aránzazu |
| title |
Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| title_short |
Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| title_full |
Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| title_fullStr |
Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| title_full_unstemmed |
Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| title_sort |
mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcus |
| publisher |
Universidad de La Rioja (España) |
| publishDate |
2002 |
| url |
https://dialnet.unirioja.es/servlet/oaites?codigo=64 |
| work_keys_str_mv |
AT portillobarrioaranzazu mecanismosderesistenciaaantibioticosmacrolidoslincosamidasyestreptograminasenstreptococcusyenterococcus |
| _version_ |
1718346545583095808 |
| spelling |
oai-TES00000000072016-04-13Mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas en streptococcus y enterococcusPortillo Barrio, AránzazuObjetive: Characterization of macrolide, lincosamide and streptogramin (MLS) resistance mechanisms in Streptococcus and Enterococcus in La Rioja, from 1996 to 2001. Distribution of erythromcyin resistance phenotypes and involved mechanisms were studied for both genera, as well as temporary evolution and clonal diversity for erythromycin-resistant Streptococcus isolates. A new gene that we called msrC was described for an enterococcal species. Conclusions: High erythromycin resistance rates were detected for beta-haemolytic group A, B, C and G Streptococcus isolates in La Rioja (13-25%) and it was even higher for S. pneumoniae isolates (54%). Percentages of erythromycin-resistant isolates decreased, from 35 to 20% and from 64 to 47%, for S. pyogenes and S. pneumoniae, respectively. M resistance phenotype was the most frequently found among erythromycin-resistant S. pyogenes isolates (80%). MLSB resistance phenotype was exclusive for beta-haemolytic Streptococcus group C and G, and it was >97% for S. agalactiae and S. pneumoniae. mef(A) or mef(E) genes, associated with M resistance phenotype, and erm(B) or erm(TR) genes, associated with MLSB resistance phenotype, were predominant in Streptococcus, depending on the species. Beta-haemolytic erythromycin-resistant Streptococcus isolates with the same clonal origin were detected in different years of the study. The erm(B) gene was detected in all erythromycin highly-resistant Enterococcus isolates, with the exception of one isolate of E. faecium which presented the erm(A) gene. A new gene that we called msrC was detected in all E. faecium isolates, independently of their erythromycin resistance pattern. The remaining species of Enterococcus analyzed did not present msrC. The msrC gene, described in E. faecium, contains 1479 bp and it has 53% identity with msr(A) gene, described in Staphylococcus and related to macrolide and streptogramin B resistance in this bacteria. The msrC gene codifies a protein of 492 amino acids and it belongs to a family of efflux pumps genes. An erythromycin efflux system, dependent on energy, was seen in E. faecium isolates with different levels of resistance to this macrolide. The efflux pump MsrC could be related to this erythromycin efflux.Objetivo: Caracterizar los mecanismos de resistencia a antibióticos macrólidos, lincosamidas y estreptograminas (MLS) en Streptococcus y Enterococcus en La Rioja, entre los años 1996 y 2001. Para ello, se estudió la distribución de fenotipos y mecanismos de resistencia en ambos géneros bacterianos, la evolución temporal y diversidad clonal de aislamientos de Streptococcus resistentes a eritromicina y se caracterizó un nuevo gen, que denominamos msrC, en una especie de Enterococcus. Conclusiones: Se detectaron altas tasas de resistencia a eritromicina en Streptococcus beta-hemolíticos de los grupos A, B, C y G en la Rioja (13-25%); ésta fue superior en S. pneumoniae (54%). El porcentaje de aislamientos resistentes a eritromicina disminuyó, del 35 al 20% y del 64 al 47%, en S. pyogenes y S. pneumoniae, respectivamente. El fenotipo M fue el más frecuente en aislamientos de S. pyogenes resistentes a eritromicina (80%). El fenotipo MLSB fue exclusivo en Streptococcus beta-hemolíticos C y G, y mayoritario en S. agalactiae y S. pneumoniae (>97%). Los genes mef(A) o mef(E), asociados al fenotipo M, y los genes erm(B) o erm(TR), asociados al fenotipo MLSB, fueron los predominantes en Streptococcus, según las especies. Se detectaron aislamientos de Streptococcus beta-hemolíticos resistentes a eritromicina pertenecientes a un mismo clon en diferentes años del estudio. El gen erm(B) fue encontrado en todas los aislamientos de Enterococcus altamente resistentes a eritromicina, a excepción de un aislamiento de E. faecium que presentó erm(A). En todos los aislamientos de E. faecium, independientemente de su fenotipo de resistencia a eritromicina, se detectó un nuevo gen que denominamos msrC. El resto de Enterococcus analizados no presentaron dicho gen. El gen msrC, descrito en E. faecium, contiene 1479 pb y posee un 53% de identidad con msr(A), relacionado en Staphylococcus con resistencia a macrólidos y estreptograminas B. El gen msrC codifica una proteína de 492 aminoácidos y pertenece a una familia de genes de bombas de eflujo. Se evidenció un sistema de bombeo de eritromicina, dependiente de energía, en aislamientos de E. faecium con diferentes niveles de resistencia a dicho macrólido. La bomba de eflujo MsrC podría estar relacionada con este bombeo de eritromicina.Universidad de La Rioja (España)Torres Manrique, Carmen (Universidad de La Rioja)Ruiz Larrea, Fernanda (Universidad de La Rioja)2002text (thesis)application/pdfhttps://dialnet.unirioja.es/servlet/oaites?codigo=64(Tesis) ISBN 84-688-2331-7 spaLICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. 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