Influência da irradiação com lasers de alta potência na microinfiltração bacteriana e fúngica em dentes humanos retrobturados com MTA
This in vitro experimental study evaluated lhe microleakage of E. faecalis and C. albicans in roots whose ressected apical surfaces and retrocavities had suffered irradiation from Nd:YAG, Diode 810 nm and Er,Cr:YSGG lasers, and later retrofiled with MTA. Seventy roots had been prepared, obturated, h...
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Formato: | text (thesis) |
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Universidade de São Paulo (USP) (Brasil)
2008
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Acceso en línea: | https://dialnet.unirioja.es/servlet/oaites?codigo=18688 |
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Sumario: | This in vitro experimental study evaluated lhe microleakage of E. faecalis and C. albicans in roots whose ressected apical surfaces and retrocavities had suffered irradiation from Nd:YAG, Diode 810 nm and Er,Cr:YSGG lasers, and later retrofiled with MTA. Seventy roots had been prepared, obturated, had suffered apicoectomy and were retroprepared with diamond and conventional ultrasonic tips in the average power. The division of specimens was in accordance with the used laser and the type of microorganism. The groups G1 and G5 had not been irradiated, G2 and G6 irradiated with Nd:YAG, G3 and G7 with Diode and G4 and G8 with Er, Cr:YSGG (n = 8). Positive (n = 3) and negative (n = 3) controls had been used. All procedures executed until this point had been carried inside an access difficulties simulation device. After the waterproofing, attachment of the roots in prepared tubes and sterilization, the retrofillings were made using white MTA. The superior chambers were inoculated with the microorganisms and inferior chambers were completed with sterile culture broth in contact with the root tip. The G1, G2, G3 and G4 groups had been inoculated with E. faeca!is and the rest of the groups with C. albicans. The contaminated broths of the upper chambers were exchanged each 3 days and for 60 days the verification of the turbidity in the inferior chambers was evaluated daily. At the end, the results had indicated that: the positive control had 100% microieakage, the negative control none and the experimental groups had presented microieakege patterns that range from 50% (G1) to 100% (G2), with a total of 68.75% of all specimens presenting microleakage. Applying the Exact Fischer test and Kruskai-Wallis test (p > 0,05), no significant statistical difference between the groups were observed in any interaction. lt can be concluded that the treatment of the retrocavities and ressected apical surface, with the lasers used in this study, did not modify the rate of E. faecalis and C. a!bicans microleakage in MTA retrofillings. Also the type of used microorganism did not seem to modify the amount of microleakage in the specimens. |
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