Estimulación de la síntesis de colágeno en cultivos celulares. Posible tratamiento de enfermedades degenerativas mediante la dieta
Osteoarthritis is the most frequent worldwide osteoarticular alteration: more than 50% of the population aged 65 and 80% of those older than 75 years suffers it. It consists of a degeneration of the cartilage joint until it disappears to expose the subchondral bone. Cartilage joint has an abundant e...
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| Formato: | text (thesis) |
| Lenguaje: | spa |
| Publicado: |
Universidad de Granada (España)
2006
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| Acceso en línea: | https://dialnet.unirioja.es/servlet/oaites?codigo=47720 |
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| Sumario: | Osteoarthritis is the most frequent worldwide osteoarticular alteration: more than 50% of the population aged 65 and 80% of those older than 75 years suffers it. It consists of a degeneration of the cartilage joint until it disappears to expose the subchondral bone.
Cartilage joint has an abundant extracellular matrix, which reaches 95% of the volume of tissue and is formed primarily by collagen, which constitutes 60% of the dry weight, and proteoglycans.
The main hypothesis of this Thesis is that the cause of osteoarthritis is a problem in collagen synthesis due to the deficiency of some amino acids. Collagen has a unique triple dextrorotatory superhelix structure, in which glycine constitutes one-third of its amino acids. In addition, the mathematical analysis of the glycine biosynthesis pathway shows that this amino acid should be considered an essential amino acid.
Fibroblasts from mouse fetuses were grown in culture media enriched with different concentrations, between 0.025 and 10 mg/mL, of glycine, proline, lysine, isoleucine, aspartic acid; as well as chondrocytes of cartilage of bovine metacarpophalangeal joints to see the effect of the different amino acids on cartilage joint collagen synthesis. Colorimetric, immunofluorescence and ELISAs techniques were used to quantify the synthesis of collagen.
The enrichment of the culture medium with glycine, proline and lysine promotes the synthesis of collagen, both in chondrocytes and fibroblasts. In cultured fibroblasts, glycine and proline exert their maximum effect within the range of physiological concentrations, while concentrations of lysine that exert the greatest stimulating effect are far from this range. As chondrocytes, three amino acids activate the synthesis of collagen with concentrations that occur in the upper area of the range of physiological concentrations.
These results indicate that concentrations of glycine, proline and lysine should be in the upper area of the range of physiological concentrations to avoid problems in the synthesis of collagen, which would be achived by supplementing the diet with these amino acids, principally glycine. |
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