Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway

Abstract Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxyge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cathrine E. Fagernes, Kåre-Olav Stensløkken, Åsmund K. Røhr, Michael Berenbrink, Stian Ellefsen, Göran E. Nilsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0002d3631a1a4460aa047a6d33fa435d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0002d3631a1a4460aa047a6d33fa435d
record_format	dspace
spelling	oai:doaj.org-article:0002d3631a1a4460aa047a6d33fa435d2021-12-02T11:53:09ZExtreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway10.1038/s41598-017-07385-42045-2322https://doaj.org/article/0002d3631a1a4460aa047a6d33fa435d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07385-4https://doaj.org/toc/2045-2322Abstract Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxygen by producing ethanol as their metabolic end-product. Here we show that this has been made possible by the evolution of a pyruvate decarboxylase, analogous to that in brewer’s yeast and the first described in vertebrates, in addition to a specialized alcohol dehydrogenase. Whole-genome duplication events have provided additional gene copies of the pyruvate dehydrogenase multienzyme complex that have evolved into a pyruvate decarboxylase, while other copies retained the essential function of the parent enzymes. We reveal the key molecular substitution in duplicated pyruvate dehydrogenase genes that underpins one of the most extreme hypoxic survival strategies among vertebrates and that is highly deleterious in humans.Cathrine E. FagernesKåre-Olav StensløkkenÅsmund K. RøhrMichael BerenbrinkStian EllefsenGöran E. NilssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cathrine E. Fagernes Kåre-Olav Stensløkken Åsmund K. Røhr Michael Berenbrink Stian Ellefsen Göran E. Nilsson Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
description	Abstract Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxygen by producing ethanol as their metabolic end-product. Here we show that this has been made possible by the evolution of a pyruvate decarboxylase, analogous to that in brewer’s yeast and the first described in vertebrates, in addition to a specialized alcohol dehydrogenase. Whole-genome duplication events have provided additional gene copies of the pyruvate dehydrogenase multienzyme complex that have evolved into a pyruvate decarboxylase, while other copies retained the essential function of the parent enzymes. We reveal the key molecular substitution in duplicated pyruvate dehydrogenase genes that underpins one of the most extreme hypoxic survival strategies among vertebrates and that is highly deleterious in humans.
format	article
author	Cathrine E. Fagernes Kåre-Olav Stensløkken Åsmund K. Røhr Michael Berenbrink Stian Ellefsen Göran E. Nilsson
author_facet	Cathrine E. Fagernes Kåre-Olav Stensløkken Åsmund K. Røhr Michael Berenbrink Stian Ellefsen Göran E. Nilsson
author_sort	Cathrine E. Fagernes
title	Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
title_short	Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
title_full	Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
title_fullStr	Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
title_full_unstemmed	Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
title_sort	extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0002d3631a1a4460aa047a6d33fa435d
work_keys_str_mv	AT cathrineefagernes extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway AT kareolavstensløkken extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway AT asmundkrøhr extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway AT michaelberenbrink extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway AT stianellefsen extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway AT goranenilsson extremeanoxiatoleranceincruciancarpandgoldfishthroughneofunctionalizationofduplicatedgenescreatinganewethanolproducingpyruvatedecarboxylasepathway
_version_	1718394846048157696

Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol-producing pyruvate decarboxylase pathway

Ejemplares similares