The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.

The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and ar...

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Autores principales:	Mohammad H Dezfulian, Danielle M Soulliere, Rajdeep K Dhaliwal, Madhulika Sareen, William L Crosby
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/000442ab5fb64411a8527b17890ec1c9
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spelling	oai:doaj.org-article:000442ab5fb64411a8527b17890ec1c92021-11-18T08:06:37ZThe SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.1932-620310.1371/journal.pone.0050984https://doaj.org/article/000442ab5fb64411a8527b17890ec1c92012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226441/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. Phylogenetic analysis based on deduced polypeptide sequence organizes the family of ASK proteins into 7 clades. The complexity of the ASK gene family, together with the close structural similarity among its members raises the prospect of significant functional redundancy among select paralogs. We have assessed the potential for functional redundancy within the ASK gene family by analyzing an expanded set of criteria that define redundancy with higher resolution. The criteria used include quantitative expression of locus-specific transcripts using qRT-PCR, assessment of the sub-cellular localization of individual ASK:YFP auto-fluorescent fusion proteins expressed in vivo as well as the in planta assessment of individual ASK-F-Box protein interactions using bimolecular fluorescent complementation techniques in combination with confocal imagery in live cells. The results indicate significant functional divergence of steady state transcript abundance and protein-protein interaction specificity involving ASK proteins in a pattern that is poorly predicted by sequence-based phylogeny. The information emerging from this and related studies will prove important for defining the functional intersection of expression, localization and gene product interaction that better predicts the formation of discrete SCF complexes, as a prelude to investigating their molecular mode of action.Mohammad H DezfulianDanielle M SoulliereRajdeep K DhaliwalMadhulika SareenWilliam L CrosbyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50984 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad H Dezfulian Danielle M Soulliere Rajdeep K Dhaliwal Madhulika Sareen William L Crosby The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
description	The Arabidopsis thaliana genome encodes several families of polypeptides that are known or predicted to participate in the formation of the SCF-class of E3-ubiquitin ligase complexes. One such gene family encodes the Skp1-like class of polypeptide subunits, where 21 genes have been identified and are known to be expressed in Arabidopsis. Phylogenetic analysis based on deduced polypeptide sequence organizes the family of ASK proteins into 7 clades. The complexity of the ASK gene family, together with the close structural similarity among its members raises the prospect of significant functional redundancy among select paralogs. We have assessed the potential for functional redundancy within the ASK gene family by analyzing an expanded set of criteria that define redundancy with higher resolution. The criteria used include quantitative expression of locus-specific transcripts using qRT-PCR, assessment of the sub-cellular localization of individual ASK:YFP auto-fluorescent fusion proteins expressed in vivo as well as the in planta assessment of individual ASK-F-Box protein interactions using bimolecular fluorescent complementation techniques in combination with confocal imagery in live cells. The results indicate significant functional divergence of steady state transcript abundance and protein-protein interaction specificity involving ASK proteins in a pattern that is poorly predicted by sequence-based phylogeny. The information emerging from this and related studies will prove important for defining the functional intersection of expression, localization and gene product interaction that better predicts the formation of discrete SCF complexes, as a prelude to investigating their molecular mode of action.
format	article
author	Mohammad H Dezfulian Danielle M Soulliere Rajdeep K Dhaliwal Madhulika Sareen William L Crosby
author_facet	Mohammad H Dezfulian Danielle M Soulliere Rajdeep K Dhaliwal Madhulika Sareen William L Crosby
author_sort	Mohammad H Dezfulian
title	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
title_short	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
title_full	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
title_fullStr	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
title_full_unstemmed	The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
title_sort	skp1-like gene family of arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/000442ab5fb64411a8527b17890ec1c9
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The SKP1-like gene family of Arabidopsis exhibits a high degree of differential gene expression and gene product interaction during development.

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