Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season

Human type A influenza viruses A(H1N1)pdm09 have caused seasonal epidemics of influenza since the 2009–2010 pandemic. A(H1N1)pdm09 viruses had a leading role in the severe epidemic season of 2015/16 in the Northern Hemisphere and caused a high incidence of acute respiratory infection (ARI) in Ukrain...

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Autores principales:	Oksana Zolotarova, Anna Fesenko, Olga Holubka, Larysa Radchenko, Eric Bortz, Iryna Budzanivska, Alla Mironenko
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	influenza H1N1 A(H1N1)pdm09 phylogenetics acute respiratory infection mutation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/00050431320b474e85cacba7cee46d59
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spelling	oai:doaj.org-article:00050431320b474e85cacba7cee46d592021-11-25T19:12:28ZGenotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season10.3390/v131121251999-4915https://doaj.org/article/00050431320b474e85cacba7cee46d592021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2125https://doaj.org/toc/1999-4915Human type A influenza viruses A(H1N1)pdm09 have caused seasonal epidemics of influenza since the 2009–2010 pandemic. A(H1N1)pdm09 viruses had a leading role in the severe epidemic season of 2015/16 in the Northern Hemisphere and caused a high incidence of acute respiratory infection (ARI) in Ukraine. Serious complications of influenza-associated severe ARI (SARI) were observed in the very young and individuals at increased risk, and 391 fatal cases occurred in the 2015/16 epidemic season. We analyzed the genetic changes in the genomes of A(H1N1)pdm09 influenza viruses isolated from SARI cases in Ukraine during the 2015/16 season. The viral hemagglutinin (HA) fell in H1 group 6B.1 for all but four isolates, with known mutations affecting glycosylation, the Sa antigenic site (S162N in all 6B.1 isolates), or virulence (D222G/N in two isolates). Other mutations occurred in antigenic site Ca (A141P and S236P), and a subgroup of four strains were in group 6B.2, with potential alterations to antigenicity in A(H1N1)pdm09 viruses circulating in 2015/16 in Ukraine. A cluster of Ukrainian isolates exhibited novel D2E and N48S mutations in the RNA binding domain, and E125D in the effector domain, of immune evasion nonstructural protein 1 (NS1). The diverse spectrum of amino-acid substitutions in HA, NS1, and other viral proteins including nucleoprotein (NP) and the polymerase complex suggested the concurrent circulation of multiple lineages of A(H1N1)pdm09 influenza viruses in the human population in Ukraine, a country with low vaccination coverage, complicating public health measures against influenza.Oksana ZolotarovaAnna FesenkoOlga HolubkaLarysa RadchenkoEric BortzIryna BudzanivskaAlla MironenkoMDPI AGarticleinfluenzaH1N1A(H1N1)pdm09phylogeneticsacute respiratory infectionmutationMicrobiologyQR1-502ENViruses, Vol 13, Iss 2125, p 2125 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	influenza H1N1 A(H1N1)pdm09 phylogenetics acute respiratory infection mutation Microbiology QR1-502
spellingShingle	influenza H1N1 A(H1N1)pdm09 phylogenetics acute respiratory infection mutation Microbiology QR1-502 Oksana Zolotarova Anna Fesenko Olga Holubka Larysa Radchenko Eric Bortz Iryna Budzanivska Alla Mironenko Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
description	Human type A influenza viruses A(H1N1)pdm09 have caused seasonal epidemics of influenza since the 2009–2010 pandemic. A(H1N1)pdm09 viruses had a leading role in the severe epidemic season of 2015/16 in the Northern Hemisphere and caused a high incidence of acute respiratory infection (ARI) in Ukraine. Serious complications of influenza-associated severe ARI (SARI) were observed in the very young and individuals at increased risk, and 391 fatal cases occurred in the 2015/16 epidemic season. We analyzed the genetic changes in the genomes of A(H1N1)pdm09 influenza viruses isolated from SARI cases in Ukraine during the 2015/16 season. The viral hemagglutinin (HA) fell in H1 group 6B.1 for all but four isolates, with known mutations affecting glycosylation, the Sa antigenic site (S162N in all 6B.1 isolates), or virulence (D222G/N in two isolates). Other mutations occurred in antigenic site Ca (A141P and S236P), and a subgroup of four strains were in group 6B.2, with potential alterations to antigenicity in A(H1N1)pdm09 viruses circulating in 2015/16 in Ukraine. A cluster of Ukrainian isolates exhibited novel D2E and N48S mutations in the RNA binding domain, and E125D in the effector domain, of immune evasion nonstructural protein 1 (NS1). The diverse spectrum of amino-acid substitutions in HA, NS1, and other viral proteins including nucleoprotein (NP) and the polymerase complex suggested the concurrent circulation of multiple lineages of A(H1N1)pdm09 influenza viruses in the human population in Ukraine, a country with low vaccination coverage, complicating public health measures against influenza.
format	article
author	Oksana Zolotarova Anna Fesenko Olga Holubka Larysa Radchenko Eric Bortz Iryna Budzanivska Alla Mironenko
author_facet	Oksana Zolotarova Anna Fesenko Olga Holubka Larysa Radchenko Eric Bortz Iryna Budzanivska Alla Mironenko
author_sort	Oksana Zolotarova
title	Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
title_short	Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
title_full	Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
title_fullStr	Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
title_full_unstemmed	Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season
title_sort	genotypic variants of pandemic h1n1 influenza a viruses isolated from severe acute respiratory infections in ukraine during the 2015/16 influenza season
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/00050431320b474e85cacba7cee46d59
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Genotypic Variants of Pandemic H1N1 Influenza A Viruses Isolated from Severe Acute Respiratory Infections in Ukraine during the 2015/16 Influenza Season

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