Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions

Abstract. Saputro TB, Muslihatin W, Wahyuni DK, Nurhidayati T, Wardhani FO, Rosalia E. 2019. Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions. Biodiversitas 20: 3299-3308. Soy...

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Autores principales:	triono bagus saputro, WIRDHATUL MUSLIHATIN, DWI KUSUMA WAHYUNI, TUTIK NURHIDAYATI, FILA OXI WARDHANI, ELENA ROSALIA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	acs, adh, gamma rays, glycine max, issr, mi, pic, sds-page, sod, waterlogging Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/001324bf37de41f981cdf662ed4f52a6
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spelling	oai:doaj.org-article:001324bf37de41f981cdf662ed4f52a62021-11-21T21:57:24ZVariation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions1412-033X2085-472210.13057/biodiv/d201124https://doaj.org/article/001324bf37de41f981cdf662ed4f52a62019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4053https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Saputro TB, Muslihatin W, Wahyuni DK, Nurhidayati T, Wardhani FO, Rosalia E. 2019. Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions. Biodiversitas 20: 3299-3308. Soybean (Glycine max L.) is the main food commodity that contains high proteins, oils, and carbohydrates. Waterlogging is considered as the dominant factor and major constraint responsible for the decrements of soybean production in Indonesia. The development of promising G.max that tolerates waterlogging is essentially needed. In this study, induction of variation was conducted by gamma rays irradiation with doses of 25Gy, 50Gy, 75Gy, and 100Gy. The mutant or variants lines were then selected under waterlogging conditions with 100%, 150%, 200%, and 250% of field capacity. The results showed that, in 250% waterlogging condition, plants irradiated with 25Gy shows the best performance in amount of roots, adventitious roots and number of pods parameters, while plants irradiated with 50Gy have the highest growth indicated by amount of root nodules, plant heights, dry weights, leaf area, and chlorophyll contents. The genotypes then were assayed by the Inter Simple Sequence Repeats (ISSR) to confirm that the mutants differ compared to its wild type. Out of 10 ISSR primers, seven primers showed polymorphic patterns. The best primer to differentiate the mutant lines and its wild type is primer ISSR1 [ (AC)8G] that able to generate the highest level of polymorphism with 44.0%. The comparison of protein profiles among the mutant lines showed that proteins with molecular weight 53,78 KDa; 43,12 KDa; and 20,62 Kda are all overexpressed for plants irradiated at 25Gy and treated with 250% waterlogging stress. All those three proteins are predicted as 1-amino cyclopropane-1-carboxylate synthase (ACS), Alcohol-dehydrogenase (ADH), and Superoxide dismutase (SOD) respectively.triono bagus saputroWIRDHATUL MUSLIHATINDWI KUSUMA WAHYUNITUTIK NURHIDAYATIFILA OXI WARDHANIELENA ROSALIAMBI & UNS Soloarticleacs, adh, gamma rays, glycine max, issr, mi, pic, sds-page, sod, waterloggingBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	acs, adh, gamma rays, glycine max, issr, mi, pic, sds-page, sod, waterlogging Biology (General) QH301-705.5
spellingShingle	acs, adh, gamma rays, glycine max, issr, mi, pic, sds-page, sod, waterlogging Biology (General) QH301-705.5 triono bagus saputro WIRDHATUL MUSLIHATIN DWI KUSUMA WAHYUNI TUTIK NURHIDAYATI FILA OXI WARDHANI ELENA ROSALIA Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
description	Abstract. Saputro TB, Muslihatin W, Wahyuni DK, Nurhidayati T, Wardhani FO, Rosalia E. 2019. Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions. Biodiversitas 20: 3299-3308. Soybean (Glycine max L.) is the main food commodity that contains high proteins, oils, and carbohydrates. Waterlogging is considered as the dominant factor and major constraint responsible for the decrements of soybean production in Indonesia. The development of promising G.max that tolerates waterlogging is essentially needed. In this study, induction of variation was conducted by gamma rays irradiation with doses of 25Gy, 50Gy, 75Gy, and 100Gy. The mutant or variants lines were then selected under waterlogging conditions with 100%, 150%, 200%, and 250% of field capacity. The results showed that, in 250% waterlogging condition, plants irradiated with 25Gy shows the best performance in amount of roots, adventitious roots and number of pods parameters, while plants irradiated with 50Gy have the highest growth indicated by amount of root nodules, plant heights, dry weights, leaf area, and chlorophyll contents. The genotypes then were assayed by the Inter Simple Sequence Repeats (ISSR) to confirm that the mutants differ compared to its wild type. Out of 10 ISSR primers, seven primers showed polymorphic patterns. The best primer to differentiate the mutant lines and its wild type is primer ISSR1 [ (AC)8G] that able to generate the highest level of polymorphism with 44.0%. The comparison of protein profiles among the mutant lines showed that proteins with molecular weight 53,78 KDa; 43,12 KDa; and 20,62 Kda are all overexpressed for plants irradiated at 25Gy and treated with 250% waterlogging stress. All those three proteins are predicted as 1-amino cyclopropane-1-carboxylate synthase (ACS), Alcohol-dehydrogenase (ADH), and Superoxide dismutase (SOD) respectively.
format	article
author	triono bagus saputro WIRDHATUL MUSLIHATIN DWI KUSUMA WAHYUNI TUTIK NURHIDAYATI FILA OXI WARDHANI ELENA ROSALIA
author_facet	triono bagus saputro WIRDHATUL MUSLIHATIN DWI KUSUMA WAHYUNI TUTIK NURHIDAYATI FILA OXI WARDHANI ELENA ROSALIA
author_sort	triono bagus saputro
title	Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
title_short	Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
title_full	Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
title_fullStr	Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
title_full_unstemmed	Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
title_sort	variation induction of glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/001324bf37de41f981cdf662ed4f52a6
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Variation induction of Glycine max through low dose gamma irradiation produces genetic and physiological alteration as source of tolerant variants in waterlogging conditions

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