The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes

Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth...

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Autores principales:	Alin Daniel Floare, Alexandra Denisa Scurtu, Octavia Iulia Balean, Doina Chioran, Roxana Buzatu, Ruxandra Sava Rosianu, Vlad Tiberiu Alexa, Daniela Jumanca, Laura-Cristina Rusu, Robert Cosmin Racea, Dorina Coricovac, Iulia Pinzaru, Cristina Adriana Dehelean, Atena Galuscan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ozone dental remineralization primary gingival fibroblasts primary gingival keratinocytes Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/001ecd00d21143c782a13cf26df389c3
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spelling	oai:doaj.org-article:001ecd00d21143c782a13cf26df389c32021-11-25T18:51:09ZThe Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes10.3390/pr91119782227-9717https://doaj.org/article/001ecd00d21143c782a13cf26df389c32021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1978https://doaj.org/toc/2227-9717Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure.Alin Daniel FloareAlexandra Denisa ScurtuOctavia Iulia BaleanDoina ChioranRoxana BuzatuRuxandra Sava RosianuVlad Tiberiu AlexaDaniela JumancaLaura-Cristina RusuRobert Cosmin RaceaDorina CoricovacIulia PinzaruCristina Adriana DeheleanAtena GaluscanMDPI AGarticleozonedental remineralizationprimary gingival fibroblastsprimary gingival keratinocytesChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1978, p 1978 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ozone dental remineralization primary gingival fibroblasts primary gingival keratinocytes Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	ozone dental remineralization primary gingival fibroblasts primary gingival keratinocytes Chemical technology TP1-1185 Chemistry QD1-999 Alin Daniel Floare Alexandra Denisa Scurtu Octavia Iulia Balean Doina Chioran Roxana Buzatu Ruxandra Sava Rosianu Vlad Tiberiu Alexa Daniela Jumanca Laura-Cristina Rusu Robert Cosmin Racea Dorina Coricovac Iulia Pinzaru Cristina Adriana Dehelean Atena Galuscan The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
description	Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure.
format	article
author	Alin Daniel Floare Alexandra Denisa Scurtu Octavia Iulia Balean Doina Chioran Roxana Buzatu Ruxandra Sava Rosianu Vlad Tiberiu Alexa Daniela Jumanca Laura-Cristina Rusu Robert Cosmin Racea Dorina Coricovac Iulia Pinzaru Cristina Adriana Dehelean Atena Galuscan
author_facet	Alin Daniel Floare Alexandra Denisa Scurtu Octavia Iulia Balean Doina Chioran Roxana Buzatu Ruxandra Sava Rosianu Vlad Tiberiu Alexa Daniela Jumanca Laura-Cristina Rusu Robert Cosmin Racea Dorina Coricovac Iulia Pinzaru Cristina Adriana Dehelean Atena Galuscan
author_sort	Alin Daniel Floare
title	The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
title_short	The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
title_full	The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
title_fullStr	The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
title_full_unstemmed	The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes
title_sort	biological effects of ozone gas on soft and hard dental tissues and the impact on human gingival fibroblasts and gingival keratinocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/001ecd00d21143c782a13cf26df389c3
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The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes

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