Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment.
Cigarette smoke (CS) protects against intestinal inflammation during ulcerative colitis. Immunoregulatory mechanisms sustaining this effect remain unknown. The aim of this study was to assess the effects of CS on experimental colitis and to characterize the intestinal inflammatory response at the ce...
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2013
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oai:doaj.org-article:001f3796374648b1956ebcf27154f5502021-11-18T07:47:50ZColonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment.1932-620310.1371/journal.pone.0062208https://doaj.org/article/001f3796374648b1956ebcf27154f5502013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638007/?tool=EBIhttps://doaj.org/toc/1932-6203Cigarette smoke (CS) protects against intestinal inflammation during ulcerative colitis. Immunoregulatory mechanisms sustaining this effect remain unknown. The aim of this study was to assess the effects of CS on experimental colitis and to characterize the intestinal inflammatory response at the cellular and molecular levels. Using the InExpose® System, a smoking device accurately reproducing human smoking habit, we pre-exposed C57BL/6 mice for 2 weeks to CS, and then we induced colitis by administration of dextran sodium sulfate (DSS). This system allowed us to demonstrate that CS exposure improved colonic inflammation (significant decrease in clinical score, body weight loss and weight/length colonic ratio). This improvement was associated with a significant decrease in colonic proinflammatory Th1/Th17 cytokine expression, as compared to unexposed mice (TNF (p=0.0169), IFNγ (p<0.0001), and IL-17 (p=0.0008)). Smoke exposure also induced an increased expression of IL-10 mRNA (p=0.0035) and a marked recruitment of iNKT (invariant Natural Killer T; CD45+ TCRβ+ CD1d tetramer+) cells in the colon of DSS-untreated mice. Demonstration of the role of iNKT cells in CS-dependent colitis improvement was performed using two different strains of NKT cells deficient mice. Indeed, in Jα18KO and CD1dKO animals, CS exposure failed to induce significant regulation of DSS-induced colitis both at the clinical and molecular levels. Thus, our study demonstrates that iNKT cells are pivotal actors in the CS-dependent protection of the colon. These results highlight the role of intestinal iNKT lymphocytes and their responsiveness to environmental stimuli. Targeting iNKT cells would represent a new therapeutic way for inflammatory bowel diseases.Muriel MontbarbonMuriel PichavantAudrey LangloisEdmone ErdualFrançois MaggiottoChristel NeutThierry MallevaeySébastien DharancyLaurent DubuquoyFrançois TrotteinAntoine CortotPierre DesreumauxPhilippe GossetBenjamin BertinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62208 (2013) |
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Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Muriel Montbarbon Muriel Pichavant Audrey Langlois Edmone Erdual François Maggiotto Christel Neut Thierry Mallevaey Sébastien Dharancy Laurent Dubuquoy François Trottein Antoine Cortot Pierre Desreumaux Philippe Gosset Benjamin Bertin Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| description |
Cigarette smoke (CS) protects against intestinal inflammation during ulcerative colitis. Immunoregulatory mechanisms sustaining this effect remain unknown. The aim of this study was to assess the effects of CS on experimental colitis and to characterize the intestinal inflammatory response at the cellular and molecular levels. Using the InExpose® System, a smoking device accurately reproducing human smoking habit, we pre-exposed C57BL/6 mice for 2 weeks to CS, and then we induced colitis by administration of dextran sodium sulfate (DSS). This system allowed us to demonstrate that CS exposure improved colonic inflammation (significant decrease in clinical score, body weight loss and weight/length colonic ratio). This improvement was associated with a significant decrease in colonic proinflammatory Th1/Th17 cytokine expression, as compared to unexposed mice (TNF (p=0.0169), IFNγ (p<0.0001), and IL-17 (p=0.0008)). Smoke exposure also induced an increased expression of IL-10 mRNA (p=0.0035) and a marked recruitment of iNKT (invariant Natural Killer T; CD45+ TCRβ+ CD1d tetramer+) cells in the colon of DSS-untreated mice. Demonstration of the role of iNKT cells in CS-dependent colitis improvement was performed using two different strains of NKT cells deficient mice. Indeed, in Jα18KO and CD1dKO animals, CS exposure failed to induce significant regulation of DSS-induced colitis both at the clinical and molecular levels. Thus, our study demonstrates that iNKT cells are pivotal actors in the CS-dependent protection of the colon. These results highlight the role of intestinal iNKT lymphocytes and their responsiveness to environmental stimuli. Targeting iNKT cells would represent a new therapeutic way for inflammatory bowel diseases. |
| format |
article |
| author |
Muriel Montbarbon Muriel Pichavant Audrey Langlois Edmone Erdual François Maggiotto Christel Neut Thierry Mallevaey Sébastien Dharancy Laurent Dubuquoy François Trottein Antoine Cortot Pierre Desreumaux Philippe Gosset Benjamin Bertin |
| author_facet |
Muriel Montbarbon Muriel Pichavant Audrey Langlois Edmone Erdual François Maggiotto Christel Neut Thierry Mallevaey Sébastien Dharancy Laurent Dubuquoy François Trottein Antoine Cortot Pierre Desreumaux Philippe Gosset Benjamin Bertin |
| author_sort |
Muriel Montbarbon |
| title |
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| title_short |
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| title_full |
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| title_fullStr |
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| title_full_unstemmed |
Colonic inflammation in mice is improved by cigarette smoke through iNKT cells recruitment. |
| title_sort |
colonic inflammation in mice is improved by cigarette smoke through inkt cells recruitment. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/001f3796374648b1956ebcf27154f550 |
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