Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard

Abstract Linoleic acid (C18:2) is an important polyunsaturated fatty acid in the seed oil of many crops. Here, we report that mutations in the promoter, intron and CDS of the FAD2 genes SalFAD2.LIA1 and SalFAD2.LIA2 generate three alleles LIA 1a , LIA 1b and lia 1 and two alleles LIA 2 and lia 2, re...

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Autores principales:	Fangqin Zeng, Vicky Roslinsky, Bifang Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0022117cd50c484683619d1df1a7b611
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spelling	oai:doaj.org-article:0022117cd50c484683619d1df1a7b6112021-12-02T15:05:40ZMutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard10.1038/s41598-017-08317-y2045-2322https://doaj.org/article/0022117cd50c484683619d1df1a7b6112017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08317-yhttps://doaj.org/toc/2045-2322Abstract Linoleic acid (C18:2) is an important polyunsaturated fatty acid in the seed oil of many crops. Here, we report that mutations in the promoter, intron and CDS of the FAD2 genes SalFAD2.LIA1 and SalFAD2.LIA2 generate three alleles LIA 1a , LIA 1b and lia 1 and two alleles LIA 2 and lia 2, respectively, controlling the C18:2 variation (4.4–32.7%) in yellow mustard. The allelic effect on increasing C18:2 content is LIA 1a > LIA 1b > lia 1 , LIA 2 > lia 2, and LIA 1a > LIA 2. The five FAD 2 alleles each contain two exons, one intron and a promoter adjacent to exon 1. LIA 1a has a 1152 bp CDS, a 1221 bp intron with promoter function and a 607 bp promoter. Compared with LIA 1a , the intron of LIA 1b has reduced promoter activity and that of LIA 2 and lia 2 has no promoter function due to extensive SNP and indel mutations. lia 1 differed from LIA 1b by having an insertion of 1223 bp retrotransposon in its intron. lia 2 with mutations in the promoter has reduced promoter activity compared with LIA 2 . This study revealed that complex quantitative variation of trait phenotype in plants could be modulated by multiple alleles of oligogenic loci resulting from mutations in the regulatory region and CDS.Fangqin ZengVicky RoslinskyBifang ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fangqin Zeng Vicky Roslinsky Bifang Cheng Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
description	Abstract Linoleic acid (C18:2) is an important polyunsaturated fatty acid in the seed oil of many crops. Here, we report that mutations in the promoter, intron and CDS of the FAD2 genes SalFAD2.LIA1 and SalFAD2.LIA2 generate three alleles LIA 1a , LIA 1b and lia 1 and two alleles LIA 2 and lia 2, respectively, controlling the C18:2 variation (4.4–32.7%) in yellow mustard. The allelic effect on increasing C18:2 content is LIA 1a > LIA 1b > lia 1 , LIA 2 > lia 2, and LIA 1a > LIA 2. The five FAD 2 alleles each contain two exons, one intron and a promoter adjacent to exon 1. LIA 1a has a 1152 bp CDS, a 1221 bp intron with promoter function and a 607 bp promoter. Compared with LIA 1a , the intron of LIA 1b has reduced promoter activity and that of LIA 2 and lia 2 has no promoter function due to extensive SNP and indel mutations. lia 1 differed from LIA 1b by having an insertion of 1223 bp retrotransposon in its intron. lia 2 with mutations in the promoter has reduced promoter activity compared with LIA 2 . This study revealed that complex quantitative variation of trait phenotype in plants could be modulated by multiple alleles of oligogenic loci resulting from mutations in the regulatory region and CDS.
format	article
author	Fangqin Zeng Vicky Roslinsky Bifang Cheng
author_facet	Fangqin Zeng Vicky Roslinsky Bifang Cheng
author_sort	Fangqin Zeng
title	Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
title_short	Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
title_full	Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
title_fullStr	Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
title_full_unstemmed	Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard
title_sort	mutations in the promoter, intron and cds of two fad2 generate multiple alleles modulating linoleic acid level in yellow mustard
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0022117cd50c484683619d1df1a7b611
work_keys_str_mv	AT fangqinzeng mutationsinthepromoterintronandcdsoftwofad2generatemultipleallelesmodulatinglinoleicacidlevelinyellowmustard AT vickyroslinsky mutationsinthepromoterintronandcdsoftwofad2generatemultipleallelesmodulatinglinoleicacidlevelinyellowmustard AT bifangcheng mutationsinthepromoterintronandcdsoftwofad2generatemultipleallelesmodulatinglinoleicacidlevelinyellowmustard
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Mutations in the promoter, intron and CDS of two FAD2 generate multiple alleles modulating linoleic acid level in yellow mustard

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