In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model

Resorbable polyglycolic acid (PGA) chondrocyte grafts are clinically established for human articular cartilage defects. Long-term implant performance was addressed in a standardized in vitro model. PGA implants (+/− bovine chondrocytes) were placed inside cartilage rings punched out of bovine femora...

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Autores principales:	Victoria Horbert, Long Xin, Peter Föhr, René Huber, Rainer H. Burgkart, Raimund W. Kinne
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bovine cartilage punch model polyglycolic acid (PGA) articular cartilage regeneration push-out test for implants Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/002264c43a764adcb4de52e775acc7cb
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spelling	oai:doaj.org-article:002264c43a764adcb4de52e775acc7cb2021-11-11T17:13:24ZIn Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model10.3390/ijms2221117691422-00671661-6596https://doaj.org/article/002264c43a764adcb4de52e775acc7cb2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11769https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Resorbable polyglycolic acid (PGA) chondrocyte grafts are clinically established for human articular cartilage defects. Long-term implant performance was addressed in a standardized in vitro model. PGA implants (+/− bovine chondrocytes) were placed inside cartilage rings punched out of bovine femoral trochleas (outer Ø 6 mm; inner defect Ø 2 mm) and cultured for 84 days (12 weeks). Cartilage/PGA hybrids were subsequently analyzed by histology (hematoxylin/eosin; safranin O), immunohistochemistry (aggrecan, collagens 1 and 2), protein assays, quantitative real-time polymerase chain reactions, and implant push-out force measurements. Cartilage/PGA hybrids remained vital with intact matrix until 12 weeks, limited loss of proteoglycans from “host” cartilage or cartilage–PGA interface, and progressively diminishing release of proteoglycans into the supernatant. By contrast, the collagen 2 content in cartilage and cartilage–PGA interface remained approximately constant during culture (with only little collagen 1). Both implants (+/− cells) displayed implant colonization and progressively increased aggrecan and collagen 2 mRNA, but significantly decreased push-out forces over time. Cell-loaded PGA showed significantly accelerated cell colonization and significantly extended deposition of aggrecan. Augmented chondrogenic differentiation in PGA and cartilage/PGA-interface for up to 84 days suggests initial cartilage regeneration. Due to the PGA resorbability, however, the model exhibits limitations in assessing the “lateral implant bonding”.Victoria HorbertLong XinPeter FöhrRené HuberRainer H. BurgkartRaimund W. KinneMDPI AGarticlebovine cartilage punch modelpolyglycolic acid (PGA)articular cartilage regenerationpush-out test for implantsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11769, p 11769 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine cartilage punch model polyglycolic acid (PGA) articular cartilage regeneration push-out test for implants Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	bovine cartilage punch model polyglycolic acid (PGA) articular cartilage regeneration push-out test for implants Biology (General) QH301-705.5 Chemistry QD1-999 Victoria Horbert Long Xin Peter Föhr René Huber Rainer H. Burgkart Raimund W. Kinne In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
description	Resorbable polyglycolic acid (PGA) chondrocyte grafts are clinically established for human articular cartilage defects. Long-term implant performance was addressed in a standardized in vitro model. PGA implants (+/− bovine chondrocytes) were placed inside cartilage rings punched out of bovine femoral trochleas (outer Ø 6 mm; inner defect Ø 2 mm) and cultured for 84 days (12 weeks). Cartilage/PGA hybrids were subsequently analyzed by histology (hematoxylin/eosin; safranin O), immunohistochemistry (aggrecan, collagens 1 and 2), protein assays, quantitative real-time polymerase chain reactions, and implant push-out force measurements. Cartilage/PGA hybrids remained vital with intact matrix until 12 weeks, limited loss of proteoglycans from “host” cartilage or cartilage–PGA interface, and progressively diminishing release of proteoglycans into the supernatant. By contrast, the collagen 2 content in cartilage and cartilage–PGA interface remained approximately constant during culture (with only little collagen 1). Both implants (+/− cells) displayed implant colonization and progressively increased aggrecan and collagen 2 mRNA, but significantly decreased push-out forces over time. Cell-loaded PGA showed significantly accelerated cell colonization and significantly extended deposition of aggrecan. Augmented chondrogenic differentiation in PGA and cartilage/PGA-interface for up to 84 days suggests initial cartilage regeneration. Due to the PGA resorbability, however, the model exhibits limitations in assessing the “lateral implant bonding”.
format	article
author	Victoria Horbert Long Xin Peter Föhr René Huber Rainer H. Burgkart Raimund W. Kinne
author_facet	Victoria Horbert Long Xin Peter Föhr René Huber Rainer H. Burgkart Raimund W. Kinne
author_sort	Victoria Horbert
title	In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
title_short	In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
title_full	In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
title_fullStr	In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
title_full_unstemmed	In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model
title_sort	in vitro cartilage regeneration with a three-dimensional polyglycolic acid (pga) implant in a bovine cartilage punch model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/002264c43a764adcb4de52e775acc7cb
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In Vitro Cartilage Regeneration with a Three-Dimensional Polyglycolic Acid (PGA) Implant in a Bovine Cartilage Punch Model

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