Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars

Simple carbohydrates are associated with the enhanced risk of cardiovascular disease and adverse changes in lipoproteins in the organism. Conversely, sugars are known to exert a stabilizing effect on biological membranes, and this effect is widely exploited in medicine and industry for cryopreservat...

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Autores principales:	Victoria Vitkova, Vesela Yordanova, Galya Staneva, Ognyan Petkov, Angelina Stoyanova-Ivanova, Krassimira Antonova, Georgi Popkirov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipid bilayers sucrose capacitance relative permittivity dipole potential membrane structure Chemical technology TP1-1185 Chemical engineering TP155-156
Acceso en línea:	https://doaj.org/article/002848a88b174eb583046ab291494167
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spelling	oai:doaj.org-article:002848a88b174eb583046ab2914941672021-11-25T18:19:46ZDielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars10.3390/membranes111108472077-0375https://doaj.org/article/002848a88b174eb583046ab2914941672021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0375/11/11/847https://doaj.org/toc/2077-0375Simple carbohydrates are associated with the enhanced risk of cardiovascular disease and adverse changes in lipoproteins in the organism. Conversely, sugars are known to exert a stabilizing effect on biological membranes, and this effect is widely exploited in medicine and industry for cryopreservation of tissues and materials. In view of elucidating molecular mechanisms involved in the interaction of mono- and disaccharides with biomimetic lipid systems, we study the alteration of dielectric properties, the degree of hydration, and the rotational order parameter and dipole potential of lipid bilayers in the presence of sugars. Frequency-dependent deformation of cell-size unilamellar lipid vesicles in alternating electric fields and fast Fourier transform electrochemical impedance spectroscopy are applied to measure the specific capacitance of phosphatidylcholine lipid bilayers in sucrose, glucose and fructose aqueous solutions. Alteration of membrane specific capacitance is reported in sucrose solutions, while preservation of membrane dielectric properties is established in the presence of glucose and fructose. We address the effect of sugars on the hydration and the rotational order parameter for 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3- phosphocholine (POPC) and 1-stearoyl-2-oleoyl-<i>sn</i>-glycero-3- phosphocholine (SOPC). An increased degree of lipid packing is reported in sucrose solutions. The obtained results provide evidence that some small carbohydrates are able to change membrane dielectric properties, structure, and order related to membrane homeostasis. The reported data are also relevant to future developments based on the response of lipid bilayers to external physical stimuli such as electric fields and temperature changes.Victoria VitkovaVesela YordanovaGalya StanevaOgnyan PetkovAngelina Stoyanova-IvanovaKrassimira AntonovaGeorgi PopkirovMDPI AGarticlelipid bilayerssucrosecapacitancerelative permittivitydipole potentialmembrane structureChemical technologyTP1-1185Chemical engineeringTP155-156ENMembranes, Vol 11, Iss 847, p 847 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid bilayers sucrose capacitance relative permittivity dipole potential membrane structure Chemical technology TP1-1185 Chemical engineering TP155-156
spellingShingle	lipid bilayers sucrose capacitance relative permittivity dipole potential membrane structure Chemical technology TP1-1185 Chemical engineering TP155-156 Victoria Vitkova Vesela Yordanova Galya Staneva Ognyan Petkov Angelina Stoyanova-Ivanova Krassimira Antonova Georgi Popkirov Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
description	Simple carbohydrates are associated with the enhanced risk of cardiovascular disease and adverse changes in lipoproteins in the organism. Conversely, sugars are known to exert a stabilizing effect on biological membranes, and this effect is widely exploited in medicine and industry for cryopreservation of tissues and materials. In view of elucidating molecular mechanisms involved in the interaction of mono- and disaccharides with biomimetic lipid systems, we study the alteration of dielectric properties, the degree of hydration, and the rotational order parameter and dipole potential of lipid bilayers in the presence of sugars. Frequency-dependent deformation of cell-size unilamellar lipid vesicles in alternating electric fields and fast Fourier transform electrochemical impedance spectroscopy are applied to measure the specific capacitance of phosphatidylcholine lipid bilayers in sucrose, glucose and fructose aqueous solutions. Alteration of membrane specific capacitance is reported in sucrose solutions, while preservation of membrane dielectric properties is established in the presence of glucose and fructose. We address the effect of sugars on the hydration and the rotational order parameter for 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3- phosphocholine (POPC) and 1-stearoyl-2-oleoyl-<i>sn</i>-glycero-3- phosphocholine (SOPC). An increased degree of lipid packing is reported in sucrose solutions. The obtained results provide evidence that some small carbohydrates are able to change membrane dielectric properties, structure, and order related to membrane homeostasis. The reported data are also relevant to future developments based on the response of lipid bilayers to external physical stimuli such as electric fields and temperature changes.
format	article
author	Victoria Vitkova Vesela Yordanova Galya Staneva Ognyan Petkov Angelina Stoyanova-Ivanova Krassimira Antonova Georgi Popkirov
author_facet	Victoria Vitkova Vesela Yordanova Galya Staneva Ognyan Petkov Angelina Stoyanova-Ivanova Krassimira Antonova Georgi Popkirov
author_sort	Victoria Vitkova
title	Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
title_short	Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
title_full	Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
title_fullStr	Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
title_full_unstemmed	Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars
title_sort	dielectric properties of phosphatidylcholine membranes and the effect of sugars
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/002848a88b174eb583046ab291494167
work_keys_str_mv	AT victoriavitkova dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT veselayordanova dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT galyastaneva dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT ognyanpetkov dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT angelinastoyanovaivanova dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT krassimiraantonova dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars AT georgipopkirov dielectricpropertiesofphosphatidylcholinemembranesandtheeffectofsugars
_version_	1718411298517024768

Dielectric Properties of Phosphatidylcholine Membranes and the Effect of Sugars

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