Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.

The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13)C-labelled glucose or glu...

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Autores principales:	Nadine Gillmaier, Andreas Götz, Anette Schulz, Wolfgang Eisenreich, Werner Goebel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0036e0b206ae4eaab3c7c16beca3ca9d
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spelling	oai:doaj.org-article:0036e0b206ae4eaab3c7c16beca3ca9d2021-11-18T08:03:55ZMetabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.1932-620310.1371/journal.pone.0052378https://doaj.org/article/0036e0b206ae4eaab3c7c16beca3ca9d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285016/?tool=EBIhttps://doaj.org/toc/1932-6203The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13)C-labelled glucose or glutamine as carbon tracers. The (13)C-profiles of protein-derived amino acids from labelled host cells and intracellular L. monocytogenes identified active metabolic pathways in the different cell types. In the primary cells, infection with live L. monocytogenes increased glycolytic activity and enhanced flux of pyruvate into the TCA cycle via pyruvate dehydrogenase and pyruvate carboxylase, while in J774A.1 cells the already high glycolytic and glutaminolytic activities hardly changed upon infection. The carbon metabolism of intracellular L. monocytogenes was similar in both host cells. Taken together, the data suggest that efficient listerial replication in the cytosol of the host cells mainly depends on the glycolytic activity of the hosts.Nadine GillmaierAndreas GötzAnette SchulzWolfgang EisenreichWerner GoebelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52378 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nadine Gillmaier Andreas Götz Anette Schulz Wolfgang Eisenreich Werner Goebel Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
description	The metabolic response of host cells, in particular of primary mammalian cells, to bacterial infections is poorly understood. Here, we compare the carbon metabolism of primary mouse macrophages and of established J774A.1 cells upon Listeria monocytogenes infection using (13)C-labelled glucose or glutamine as carbon tracers. The (13)C-profiles of protein-derived amino acids from labelled host cells and intracellular L. monocytogenes identified active metabolic pathways in the different cell types. In the primary cells, infection with live L. monocytogenes increased glycolytic activity and enhanced flux of pyruvate into the TCA cycle via pyruvate dehydrogenase and pyruvate carboxylase, while in J774A.1 cells the already high glycolytic and glutaminolytic activities hardly changed upon infection. The carbon metabolism of intracellular L. monocytogenes was similar in both host cells. Taken together, the data suggest that efficient listerial replication in the cytosol of the host cells mainly depends on the glycolytic activity of the hosts.
format	article
author	Nadine Gillmaier Andreas Götz Anette Schulz Wolfgang Eisenreich Werner Goebel
author_facet	Nadine Gillmaier Andreas Götz Anette Schulz Wolfgang Eisenreich Werner Goebel
author_sort	Nadine Gillmaier
title	Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
title_short	Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
title_full	Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
title_fullStr	Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
title_full_unstemmed	Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.
title_sort	metabolic responses of primary and transformed cells to intracellular listeria monocytogenes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0036e0b206ae4eaab3c7c16beca3ca9d
work_keys_str_mv	AT nadinegillmaier metabolicresponsesofprimaryandtransformedcellstointracellularlisteriamonocytogenes AT andreasgotz metabolicresponsesofprimaryandtransformedcellstointracellularlisteriamonocytogenes AT anetteschulz metabolicresponsesofprimaryandtransformedcellstointracellularlisteriamonocytogenes AT wolfgangeisenreich metabolicresponsesofprimaryandtransformedcellstointracellularlisteriamonocytogenes AT wernergoebel metabolicresponsesofprimaryandtransformedcellstointracellularlisteriamonocytogenes
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Metabolic responses of primary and transformed cells to intracellular Listeria monocytogenes.

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