Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection

Abstract Acinetobacter baumannii is a serious health threat with a high mortality rate. We have already reported prophylactic effects of IgYs raised against OmpA and Omp34 as well as against inactivated whole-cell (IWC) of A. baumannii in a murine pneumonia model. However, the infection was exacerba...

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Autores principales:	Abolfazl Jahangiri, Parviz Owlia, Iraj Rasooli, Jafar Salimian, Ehsan Derakhshanifar, Zahra Aghajani, Sajad Abdollahi, Saeed Khalili, Daryush Talei, Elham Darzi Eslam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/003868d4bba647bc94e9931e3ffa91af
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spelling	oai:doaj.org-article:003868d4bba647bc94e9931e3ffa91af2021-12-02T11:50:40ZSpecific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection10.1038/s41598-021-81356-82045-2322https://doaj.org/article/003868d4bba647bc94e9931e3ffa91af2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81356-8https://doaj.org/toc/2045-2322Abstract Acinetobacter baumannii is a serious health threat with a high mortality rate. We have already reported prophylactic effects of IgYs raised against OmpA and Omp34 as well as against inactivated whole-cell (IWC) of A. baumannii in a murine pneumonia model. However, the infection was exacerbated in the mice group that received IgYs raised against the combination of OmpA and Omp34. The current study was conducted to propose reasons for the observed antibody-dependent enhancement (ADE) in addition to the therapeutic effect of specific IgYs in the murine pneumonia model. This phenomenon was hypothetically attributed to topologically inaccessible similar epitopes of OmpA and Omp34 sharing similarity with peptides of mice proteins. In silico analyses revealed that some inaccessible peptides of OmpA shared similarity with peptides of Omp34 and Mus musculus. Specific anti-OmpA and anti-Omp34 IgYs cross-reacted with Omp34 and OmpA respectively. Specific IgYs showed different protectivity against A. baumannii AbI101 in the murine pneumonia model. IgYs triggered against OmpA or IWC of A. baumannii were the most protective antibodies. IgY triggered against Omp34 is ranked next after those against OmpA. The lowest protection was observed in mice received IgYs raised against the combination of rOmpA and rOmp34. In conclusion, specific IgYs against OmpA, Omp34, and IWC of A. baumannii could serve as novel biotherapeutics against A. baumannii pneumonia.Abolfazl JahangiriParviz OwliaIraj RasooliJafar SalimianEhsan DerakhshanifarZahra AghajaniSajad AbdollahiSaeed KhaliliDaryush TaleiElham Darzi EslamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abolfazl Jahangiri Parviz Owlia Iraj Rasooli Jafar Salimian Ehsan Derakhshanifar Zahra Aghajani Sajad Abdollahi Saeed Khalili Daryush Talei Elham Darzi Eslam Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
description	Abstract Acinetobacter baumannii is a serious health threat with a high mortality rate. We have already reported prophylactic effects of IgYs raised against OmpA and Omp34 as well as against inactivated whole-cell (IWC) of A. baumannii in a murine pneumonia model. However, the infection was exacerbated in the mice group that received IgYs raised against the combination of OmpA and Omp34. The current study was conducted to propose reasons for the observed antibody-dependent enhancement (ADE) in addition to the therapeutic effect of specific IgYs in the murine pneumonia model. This phenomenon was hypothetically attributed to topologically inaccessible similar epitopes of OmpA and Omp34 sharing similarity with peptides of mice proteins. In silico analyses revealed that some inaccessible peptides of OmpA shared similarity with peptides of Omp34 and Mus musculus. Specific anti-OmpA and anti-Omp34 IgYs cross-reacted with Omp34 and OmpA respectively. Specific IgYs showed different protectivity against A. baumannii AbI101 in the murine pneumonia model. IgYs triggered against OmpA or IWC of A. baumannii were the most protective antibodies. IgY triggered against Omp34 is ranked next after those against OmpA. The lowest protection was observed in mice received IgYs raised against the combination of rOmpA and rOmp34. In conclusion, specific IgYs against OmpA, Omp34, and IWC of A. baumannii could serve as novel biotherapeutics against A. baumannii pneumonia.
format	article
author	Abolfazl Jahangiri Parviz Owlia Iraj Rasooli Jafar Salimian Ehsan Derakhshanifar Zahra Aghajani Sajad Abdollahi Saeed Khalili Daryush Talei Elham Darzi Eslam
author_facet	Abolfazl Jahangiri Parviz Owlia Iraj Rasooli Jafar Salimian Ehsan Derakhshanifar Zahra Aghajani Sajad Abdollahi Saeed Khalili Daryush Talei Elham Darzi Eslam
author_sort	Abolfazl Jahangiri
title	Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
title_short	Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
title_full	Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
title_fullStr	Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
title_full_unstemmed	Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection
title_sort	specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against acinetobacter baumannii pneumonia infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/003868d4bba647bc94e9931e3ffa91af
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Specific egg yolk immunoglobulin as a promising non-antibiotic biotherapeutic product against Acinetobacter baumannii pneumonia infection

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