Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae)
Abstract East Africa is a hotspot of biodiversity of many orthopteran taxa, including bushcrickets. Gonatoxia Karsch, 1889 species are fully alate Phaneropterinae, which are perfectly adapted to the foliage of forests. We examined five species using combined cytogenetic and molecular data to determi...
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2021
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oai:doaj.org-article:003f3a32dcd04e8f8b794a3c1885dc722021-11-28T12:15:34ZHighly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae)10.1038/s41598-021-02110-82045-2322https://doaj.org/article/003f3a32dcd04e8f8b794a3c1885dc722021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02110-8https://doaj.org/toc/2045-2322Abstract East Africa is a hotspot of biodiversity of many orthopteran taxa, including bushcrickets. Gonatoxia Karsch, 1889 species are fully alate Phaneropterinae, which are perfectly adapted to the foliage of forests. We examined five species using combined cytogenetic and molecular data to determine the inter- and intraspecific genetic diversity. The variation in the diploid number of chromosomes in males ranged from 2n = 28 + X0 and 26 + X0 to 2n = 6 + X0. Fluorescence in situ hybridization showed from one to many 18S rDNA loci as well as interstitial sequences, especially in G. helleri. 18S rDNA loci coincided with active NOR and C-banding patterns. The isolation of populations of the species explains differences in the number of chromosomes (G. maculata), chromosomal polymorphism and chromosomal heterozygosity (G. helleri). Our molecular phylogeny based on the COI locus supported the monophyly of the genus Gonatoxia and separateness of the five examined species in accordance with their morphological features and chromosome numbers as well as the species’ distribution.Elżbieta Warchałowska-ŚliwaBeata GrzywaczMaciej KocińskiAnna Maryańska-NadachowskaKlaus-Gerhard HellerClaudia HempNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Elżbieta Warchałowska-Śliwa Beata Grzywacz Maciej Kociński Anna Maryańska-Nadachowska Klaus-Gerhard Heller Claudia Hemp Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| description |
Abstract East Africa is a hotspot of biodiversity of many orthopteran taxa, including bushcrickets. Gonatoxia Karsch, 1889 species are fully alate Phaneropterinae, which are perfectly adapted to the foliage of forests. We examined five species using combined cytogenetic and molecular data to determine the inter- and intraspecific genetic diversity. The variation in the diploid number of chromosomes in males ranged from 2n = 28 + X0 and 26 + X0 to 2n = 6 + X0. Fluorescence in situ hybridization showed from one to many 18S rDNA loci as well as interstitial sequences, especially in G. helleri. 18S rDNA loci coincided with active NOR and C-banding patterns. The isolation of populations of the species explains differences in the number of chromosomes (G. maculata), chromosomal polymorphism and chromosomal heterozygosity (G. helleri). Our molecular phylogeny based on the COI locus supported the monophyly of the genus Gonatoxia and separateness of the five examined species in accordance with their morphological features and chromosome numbers as well as the species’ distribution. |
| format |
article |
| author |
Elżbieta Warchałowska-Śliwa Beata Grzywacz Maciej Kociński Anna Maryańska-Nadachowska Klaus-Gerhard Heller Claudia Hemp |
| author_facet |
Elżbieta Warchałowska-Śliwa Beata Grzywacz Maciej Kociński Anna Maryańska-Nadachowska Klaus-Gerhard Heller Claudia Hemp |
| author_sort |
Elżbieta Warchałowska-Śliwa |
| title |
Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| title_short |
Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| title_full |
Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| title_fullStr |
Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| title_full_unstemmed |
Highly divergent karyotypes and barcoding of the East African genus Gonatoxia Karsch (Orthoptera: Phaneropterinae) |
| title_sort |
highly divergent karyotypes and barcoding of the east african genus gonatoxia karsch (orthoptera: phaneropterinae) |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/003f3a32dcd04e8f8b794a3c1885dc72 |
| work_keys_str_mv |
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| _version_ |
1718408056310595584 |