Interferometric rotating point spread function

Interferometric rotating point spread function

Abstract Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4...

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Autores principales: Wei Wang, Guohai Situ
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/00435f14fa714b9694c13b2beae98b12
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spelling oai:doaj.org-article:00435f14fa714b9694c13b2beae98b122021-12-02T16:06:22ZInterferometric rotating point spread function10.1038/s41598-017-06203-12045-2322https://doaj.org/article/00435f14fa714b9694c13b2beae98b122017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06203-1https://doaj.org/toc/2045-2322Abstract Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is transferred to the azimuthal rotation. Moreover, we design an iDH PSF whose imaging range reaches 3 μm, which is roughly 3 times as much as that which can be obtained by using other interferometric localization-based super-resolution imaging methods.Wei WangGuohai SituNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Wei Wang
Guohai Situ
Interferometric rotating point spread function
description Abstract Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of their large working depth range. In this article, we propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is transferred to the azimuthal rotation. Moreover, we design an iDH PSF whose imaging range reaches 3 μm, which is roughly 3 times as much as that which can be obtained by using other interferometric localization-based super-resolution imaging methods.
format article
author Wei Wang
Guohai Situ
author_facet Wei Wang
Guohai Situ
author_sort Wei Wang
title Interferometric rotating point spread function
title_short Interferometric rotating point spread function
title_full Interferometric rotating point spread function
title_fullStr Interferometric rotating point spread function
title_full_unstemmed Interferometric rotating point spread function
title_sort interferometric rotating point spread function
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/00435f14fa714b9694c13b2beae98b12
work_keys_str_mv AT weiwang interferometricrotatingpointspreadfunction
AT guohaisitu interferometricrotatingpointspreadfunction
_version_ 1718385003555979264

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