The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic

Evolution: Bacteria gain advantageous mutations under simulated microgravity Bacteria grown for an extended period of time under simulated microgravity adopt growth advantages. George Fox and colleagues from the University of Houston, Texas, USA, cultured Escherichia coli bacteria for 1000 generatio...

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Autores principales:	Madhan R. Tirumalai, Fathi Karouia, Quyen Tran, Victor G. Stepanov, Rebekah J. Bruce, C. Mark Ott, Duane L. Pierson, George E. Fox
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/00446c7756a24031b3ea4ce32713b3c5
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spelling	oai:doaj.org-article:00446c7756a24031b3ea4ce32713b3c52021-12-02T11:51:09ZThe adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic10.1038/s41526-017-0020-12373-8065https://doaj.org/article/00446c7756a24031b3ea4ce32713b3c52017-05-01T00:00:00Zhttps://doi.org/10.1038/s41526-017-0020-1https://doaj.org/toc/2373-8065Evolution: Bacteria gain advantageous mutations under simulated microgravity Bacteria grown for an extended period of time under simulated microgravity adopt growth advantages. George Fox and colleagues from the University of Houston, Texas, USA, cultured Escherichia coli bacteria for 1000 generations in a high aspect rotating vessel to simulate the low fluid shear microgravity environment encountered during spaceflight. They then performed growth competition assays and found that the 1000-generation adapted bacteria outcompeted control bacteria grown without simulated microgravity. Genomic sequencing of the adapted bacteria revealed 16 mutations, five of which altered protein sequences. These DNA changes likely explain the growth advantage of the bacteria grown for multiple generations in simulated microgravity. Similar adaptations during prolonged space missions could result in nastier pathogens that might threaten the health of astronauts. Fortunately, the microbes did not appear to acquire antibiotic resistance over the 1000 generation in the modeled microgravity culture.Madhan R. TirumalaiFathi KarouiaQuyen TranVictor G. StepanovRebekah J. BruceC. Mark OttDuane L. PiersonGeorge E. FoxNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 3, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Madhan R. Tirumalai Fathi Karouia Quyen Tran Victor G. Stepanov Rebekah J. Bruce C. Mark Ott Duane L. Pierson George E. Fox The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
description	Evolution: Bacteria gain advantageous mutations under simulated microgravity Bacteria grown for an extended period of time under simulated microgravity adopt growth advantages. George Fox and colleagues from the University of Houston, Texas, USA, cultured Escherichia coli bacteria for 1000 generations in a high aspect rotating vessel to simulate the low fluid shear microgravity environment encountered during spaceflight. They then performed growth competition assays and found that the 1000-generation adapted bacteria outcompeted control bacteria grown without simulated microgravity. Genomic sequencing of the adapted bacteria revealed 16 mutations, five of which altered protein sequences. These DNA changes likely explain the growth advantage of the bacteria grown for multiple generations in simulated microgravity. Similar adaptations during prolonged space missions could result in nastier pathogens that might threaten the health of astronauts. Fortunately, the microbes did not appear to acquire antibiotic resistance over the 1000 generation in the modeled microgravity culture.
format	article
author	Madhan R. Tirumalai Fathi Karouia Quyen Tran Victor G. Stepanov Rebekah J. Bruce C. Mark Ott Duane L. Pierson George E. Fox
author_facet	Madhan R. Tirumalai Fathi Karouia Quyen Tran Victor G. Stepanov Rebekah J. Bruce C. Mark Ott Duane L. Pierson George E. Fox
author_sort	Madhan R. Tirumalai
title	The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
title_short	The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
title_full	The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
title_fullStr	The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
title_full_unstemmed	The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
title_sort	adaptation of escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/00446c7756a24031b3ea4ce32713b3c5
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The adaptation of Escherichia coli cells grown in simulated microgravity for an extended period is both phenotypic and genomic

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