CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells

Abstract Generation of pancreatic β cells from pluripotent stem cells is a key technology to develop cell therapy for insulin-dependent diabetes and considerable efforts have been made to produce β cells. However, due to multiple and lengthy differentiation steps, production of β cells is often unst...

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Autores principales:	Ami Watanabe, Anna Tanaka, Chizuko Koga, Masahito Matsumoto, Yasushi Okazaki, Tatsuya Kin, Atsushi Miyajima
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0045ce09fa434bfe9dd473e76af13f0e
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spelling	oai:doaj.org-article:0045ce09fa434bfe9dd473e76af13f0e2021-12-02T15:38:11ZCD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells10.1038/s41598-021-88978-y2045-2322https://doaj.org/article/0045ce09fa434bfe9dd473e76af13f0e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88978-yhttps://doaj.org/toc/2045-2322Abstract Generation of pancreatic β cells from pluripotent stem cells is a key technology to develop cell therapy for insulin-dependent diabetes and considerable efforts have been made to produce β cells. However, due to multiple and lengthy differentiation steps, production of β cells is often unstable. It is also desirable to eliminate undifferentiated cells to avoid potential risks of tumorigenesis. To isolate β cell precursors from late stage pancreatic endocrine progenitor (EP) cells derived from iPS cells, we have identified CD82, a member of the tetraspanin family. CD82+ cells at the EP stage differentiated into endocrine cells more efficiently than CD82− EP stage cells. We also show that CD82+ cells in human islets secreted insulin more efficiently than CD82− cells. Furthermore, knockdown of CD82 expression by siRNA or inhibition of CD82 by monoclonal antibodies in NGN3+ cells suppressed the function of β cells with glucose-stimulated insulin secretion, suggesting that CD82 plays a role in maturation of EP cells to β cells.Ami WatanabeAnna TanakaChizuko KogaMasahito MatsumotoYasushi OkazakiTatsuya KinAtsushi MiyajimaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ami Watanabe Anna Tanaka Chizuko Koga Masahito Matsumoto Yasushi Okazaki Tatsuya Kin Atsushi Miyajima CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
description	Abstract Generation of pancreatic β cells from pluripotent stem cells is a key technology to develop cell therapy for insulin-dependent diabetes and considerable efforts have been made to produce β cells. However, due to multiple and lengthy differentiation steps, production of β cells is often unstable. It is also desirable to eliminate undifferentiated cells to avoid potential risks of tumorigenesis. To isolate β cell precursors from late stage pancreatic endocrine progenitor (EP) cells derived from iPS cells, we have identified CD82, a member of the tetraspanin family. CD82+ cells at the EP stage differentiated into endocrine cells more efficiently than CD82− EP stage cells. We also show that CD82+ cells in human islets secreted insulin more efficiently than CD82− cells. Furthermore, knockdown of CD82 expression by siRNA or inhibition of CD82 by monoclonal antibodies in NGN3+ cells suppressed the function of β cells with glucose-stimulated insulin secretion, suggesting that CD82 plays a role in maturation of EP cells to β cells.
format	article
author	Ami Watanabe Anna Tanaka Chizuko Koga Masahito Matsumoto Yasushi Okazaki Tatsuya Kin Atsushi Miyajima
author_facet	Ami Watanabe Anna Tanaka Chizuko Koga Masahito Matsumoto Yasushi Okazaki Tatsuya Kin Atsushi Miyajima
author_sort	Ami Watanabe
title	CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
title_short	CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
title_full	CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
title_fullStr	CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
title_full_unstemmed	CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells
title_sort	cd82 is a marker to isolate β cell precursors from human ips cells and plays a role for the maturation of β cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0045ce09fa434bfe9dd473e76af13f0e
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CD82 is a marker to isolate β cell precursors from human iPS cells and plays a role for the maturation of β cells

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