CrAssphage and its bacterial host in cat feces

Abstract CrAssphages are a diverse group of related phages detected in human feces where they are the most prevalent and abundant prokaryotic virus. CrAssphages’ cellular host has been identified as the anaerobic Bacteroides intestinalis. CrAssphage has also been reported in non-human primates and e...

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Autores principales:	Yanpeng Li, Emilia Gordon, Ryan C. Shean, Amanda Idle, Xutao Deng, Alexander L. Greninger, Eric Delwart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00493a7ec1c4467595d14ae13bb6f503
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spelling	oai:doaj.org-article:00493a7ec1c4467595d14ae13bb6f5032021-12-02T15:23:06ZCrAssphage and its bacterial host in cat feces10.1038/s41598-020-80076-92045-2322https://doaj.org/article/00493a7ec1c4467595d14ae13bb6f5032021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80076-9https://doaj.org/toc/2045-2322Abstract CrAssphages are a diverse group of related phages detected in human feces where they are the most prevalent and abundant prokaryotic virus. CrAssphages’ cellular host has been identified as the anaerobic Bacteroides intestinalis. CrAssphage has also been reported in non-human primates and environmental samples and has been proposed as a marker of human fecal contamination. Here we describe crAssphage DNA in a feline fecal sample. 95% of the ~ 100 Kb genome could be assembled and classified in genus 1 of the recently proposed Alphacrassvirinae subfamily. The cat origin of the fecal sample was confirmed by partial mitochondrial DNA sequencing. High levels of Bacteroides intestinalis DNA could also be detected in this cat’s feces. Fecal samples longitudinally collected over a 4-week period showed the continuous shedding of crAssphage DNA. We therefore report the first genome sequence-confirmed detection of crAssphage in fecal samples of a non-primate mammal.Yanpeng LiEmilia GordonRyan C. SheanAmanda IdleXutao DengAlexander L. GreningerEric DelwartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanpeng Li Emilia Gordon Ryan C. Shean Amanda Idle Xutao Deng Alexander L. Greninger Eric Delwart CrAssphage and its bacterial host in cat feces
description	Abstract CrAssphages are a diverse group of related phages detected in human feces where they are the most prevalent and abundant prokaryotic virus. CrAssphages’ cellular host has been identified as the anaerobic Bacteroides intestinalis. CrAssphage has also been reported in non-human primates and environmental samples and has been proposed as a marker of human fecal contamination. Here we describe crAssphage DNA in a feline fecal sample. 95% of the ~ 100 Kb genome could be assembled and classified in genus 1 of the recently proposed Alphacrassvirinae subfamily. The cat origin of the fecal sample was confirmed by partial mitochondrial DNA sequencing. High levels of Bacteroides intestinalis DNA could also be detected in this cat’s feces. Fecal samples longitudinally collected over a 4-week period showed the continuous shedding of crAssphage DNA. We therefore report the first genome sequence-confirmed detection of crAssphage in fecal samples of a non-primate mammal.
format	article
author	Yanpeng Li Emilia Gordon Ryan C. Shean Amanda Idle Xutao Deng Alexander L. Greninger Eric Delwart
author_facet	Yanpeng Li Emilia Gordon Ryan C. Shean Amanda Idle Xutao Deng Alexander L. Greninger Eric Delwart
author_sort	Yanpeng Li
title	CrAssphage and its bacterial host in cat feces
title_short	CrAssphage and its bacterial host in cat feces
title_full	CrAssphage and its bacterial host in cat feces
title_fullStr	CrAssphage and its bacterial host in cat feces
title_full_unstemmed	CrAssphage and its bacterial host in cat feces
title_sort	crassphage and its bacterial host in cat feces
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/00493a7ec1c4467595d14ae13bb6f503
work_keys_str_mv	AT yanpengli crassphageanditsbacterialhostincatfeces AT emiliagordon crassphageanditsbacterialhostincatfeces AT ryancshean crassphageanditsbacterialhostincatfeces AT amandaidle crassphageanditsbacterialhostincatfeces AT xutaodeng crassphageanditsbacterialhostincatfeces AT alexanderlgreninger crassphageanditsbacterialhostincatfeces AT ericdelwart crassphageanditsbacterialhostincatfeces
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CrAssphage and its bacterial host in cat feces

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