Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

Abstract The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal prog...

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Autores principales:	S. Marozin, B. Simon-Nobbe, S. Irausek, L. W. K. Chung, G. Lepperdinger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/004ee85307cc48c09f62539ad1ee1a48
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spelling	oai:doaj.org-article:004ee85307cc48c09f62539ad1ee1a482021-12-02T16:53:00ZKinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells10.1038/s41598-021-90161-22045-2322https://doaj.org/article/004ee85307cc48c09f62539ad1ee1a482021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90161-2https://doaj.org/toc/2045-2322Abstract The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal progenitor cells and used to investigate bone-metabolism in vitro. Hereby, we studied whether and to what extent the conditionally immortalized cell line hFOB 1.19 can serve as a surrogate model for bone-marrow derived mesenchymal stromal cells (bmMSC). hFOB indeed exhibit specific characteristics reminiscent of bmMSC, as colony formation, migration capacity and the propensity to grow as multicellular aggregates. After prolonged culture, in contrast to the expected effect of immortalization, hFOB acquired a delayed growth rate. In close resemblance to bmMSC at increasing passages, also hFOB showed morphological abnormalities, enlargement and finally reduced proliferation rates together with enhanced expression of the cell cycle inhibitors p21 and p16. hFOB not only have the ability to undergo multilineage differentiation but portray several important aspects of human bone marrow mesenchymal stromal cells. Superior to primary MSC and osteoblasts, hFOB enabled the generation of continuous cell lines. These provide an advanced basis for investigating age-related dysfunctions of MSCs in an in vitro 3D-stem cell microenvironment.S. MarozinB. Simon-NobbeS. IrausekL. W. K. ChungG. LepperdingerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Marozin B. Simon-Nobbe S. Irausek L. W. K. Chung G. Lepperdinger Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
description	Abstract The human fetal osteoblast cell line (hFOB 1.19) has been proposed as an accessible experimental model for study of osteoblast biology relating to drug development and biomaterial engineering. For their multilineage differentiation potential, hFOB has been compared to human mesenchymal progenitor cells and used to investigate bone-metabolism in vitro. Hereby, we studied whether and to what extent the conditionally immortalized cell line hFOB 1.19 can serve as a surrogate model for bone-marrow derived mesenchymal stromal cells (bmMSC). hFOB indeed exhibit specific characteristics reminiscent of bmMSC, as colony formation, migration capacity and the propensity to grow as multicellular aggregates. After prolonged culture, in contrast to the expected effect of immortalization, hFOB acquired a delayed growth rate. In close resemblance to bmMSC at increasing passages, also hFOB showed morphological abnormalities, enlargement and finally reduced proliferation rates together with enhanced expression of the cell cycle inhibitors p21 and p16. hFOB not only have the ability to undergo multilineage differentiation but portray several important aspects of human bone marrow mesenchymal stromal cells. Superior to primary MSC and osteoblasts, hFOB enabled the generation of continuous cell lines. These provide an advanced basis for investigating age-related dysfunctions of MSCs in an in vitro 3D-stem cell microenvironment.
format	article
author	S. Marozin B. Simon-Nobbe S. Irausek L. W. K. Chung G. Lepperdinger
author_facet	S. Marozin B. Simon-Nobbe S. Irausek L. W. K. Chung G. Lepperdinger
author_sort	S. Marozin
title	Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
title_short	Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
title_full	Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
title_fullStr	Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
title_full_unstemmed	Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
title_sort	kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/004ee85307cc48c09f62539ad1ee1a48
work_keys_str_mv	AT smarozin kinshipofconditionallyimmortalizedcellsderivedfromfetalbonetohumanbonederivedmesenchymalstromacells AT bsimonnobbe kinshipofconditionallyimmortalizedcellsderivedfromfetalbonetohumanbonederivedmesenchymalstromacells AT sirausek kinshipofconditionallyimmortalizedcellsderivedfromfetalbonetohumanbonederivedmesenchymalstromacells AT lwkchung kinshipofconditionallyimmortalizedcellsderivedfromfetalbonetohumanbonederivedmesenchymalstromacells AT glepperdinger kinshipofconditionallyimmortalizedcellsderivedfromfetalbonetohumanbonederivedmesenchymalstromacells
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Kinship of conditionally immortalized cells derived from fetal bone to human bone-derived mesenchymal stroma cells

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