A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes

Ubiquitin (Ub) and Ub-like modifiers (Ubls) can form chains of various topologies, but preparing defined chains for functional studies remains challenging. Here, the authors develop chemoenzymatic tools to tailormake Ub/Ubl chains and study the involvement of specific Ub/SUMO chains in DNA repair.

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Autores principales:	Maximilian Fottner, Maria Weyh, Stefan Gaussmann, Dominic Schwarz, Michael Sattler, Kathrin Lang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0060470e838f4de9846b528a91c52b76
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id	oai:doaj.org-article:0060470e838f4de9846b528a91c52b76
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spelling	oai:doaj.org-article:0060470e838f4de9846b528a91c52b762021-11-14T12:34:29ZA modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes10.1038/s41467-021-26812-92041-1723https://doaj.org/article/0060470e838f4de9846b528a91c52b762021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26812-9https://doaj.org/toc/2041-1723Ubiquitin (Ub) and Ub-like modifiers (Ubls) can form chains of various topologies, but preparing defined chains for functional studies remains challenging. Here, the authors develop chemoenzymatic tools to tailormake Ub/Ubl chains and study the involvement of specific Ub/SUMO chains in DNA repair.Maximilian FottnerMaria WeyhStefan GaussmannDominic SchwarzMichael SattlerKathrin LangNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maximilian Fottner Maria Weyh Stefan Gaussmann Dominic Schwarz Michael Sattler Kathrin Lang A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
description	Ubiquitin (Ub) and Ub-like modifiers (Ubls) can form chains of various topologies, but preparing defined chains for functional studies remains challenging. Here, the authors develop chemoenzymatic tools to tailormake Ub/Ubl chains and study the involvement of specific Ub/SUMO chains in DNA repair.
format	article
author	Maximilian Fottner Maria Weyh Stefan Gaussmann Dominic Schwarz Michael Sattler Kathrin Lang
author_facet	Maximilian Fottner Maria Weyh Stefan Gaussmann Dominic Schwarz Michael Sattler Kathrin Lang
author_sort	Maximilian Fottner
title	A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
title_short	A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
title_full	A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
title_fullStr	A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
title_full_unstemmed	A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
title_sort	modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0060470e838f4de9846b528a91c52b76
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A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes

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