Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compare...

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Autores principales:	Yumiko Hirokawa, Jordan Clarke, Michelle Palmieri, Tao Tan, Dmitri Mouradov, Shan Li, Cong Lin, Fuqiang Li, Huijuan Luo, Kui Wu, Maree Faux, Chin Wee Tan, Margaret Lee, Grace Gard, Peter Gibbs, Antony W. Burgess, Oliver M. Sieber
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/006bc56a251a435dbc12018d048e3d9b
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spelling	oai:doaj.org-article:006bc56a251a435dbc12018d048e3d9b2021-12-02T17:25:42ZLow-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine10.1038/s42003-021-02607-y2399-3642https://doaj.org/article/006bc56a251a435dbc12018d048e3d9b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02607-yhttps://doaj.org/toc/2399-3642Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compared to the existing dome method, while maintaining similar morphology, and represents a useful approach for high-throughput applications of organoids.Yumiko HirokawaJordan ClarkeMichelle PalmieriTao TanDmitri MouradovShan LiCong LinFuqiang LiHuijuan LuoKui WuMaree FauxChin Wee TanMargaret LeeGrace GardPeter GibbsAntony W. BurgessOliver M. SieberNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Yumiko Hirokawa Jordan Clarke Michelle Palmieri Tao Tan Dmitri Mouradov Shan Li Cong Lin Fuqiang Li Huijuan Luo Kui Wu Maree Faux Chin Wee Tan Margaret Lee Grace Gard Peter Gibbs Antony W. Burgess Oliver M. Sieber Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
description	Given the practical limitations of solid matrix-based protocols in organoid culture, Yumiko Hirokawa et al. assess the ability of low-concentration Matrigel conditions to promote intestinal organoid growth. Their results suggest that a low-viscosity culture system can improve live cell yield compared to the existing dome method, while maintaining similar morphology, and represents a useful approach for high-throughput applications of organoids.
format	article
author	Yumiko Hirokawa Jordan Clarke Michelle Palmieri Tao Tan Dmitri Mouradov Shan Li Cong Lin Fuqiang Li Huijuan Luo Kui Wu Maree Faux Chin Wee Tan Margaret Lee Grace Gard Peter Gibbs Antony W. Burgess Oliver M. Sieber
author_facet	Yumiko Hirokawa Jordan Clarke Michelle Palmieri Tao Tan Dmitri Mouradov Shan Li Cong Lin Fuqiang Li Huijuan Luo Kui Wu Maree Faux Chin Wee Tan Margaret Lee Grace Gard Peter Gibbs Antony W. Burgess Oliver M. Sieber
author_sort	Yumiko Hirokawa
title	Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
title_short	Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
title_full	Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
title_fullStr	Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
title_full_unstemmed	Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
title_sort	low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/006bc56a251a435dbc12018d048e3d9b
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Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

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