Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.

Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy. We have developed a filter system to excite fluor...

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Autores principales:	Kazuo Yamagata, Daisaku Iwamoto, Yukari Terashita, Chong Li, Sayaka Wakayama, Yoko Hayashi-Takanaka, Hiroshi Kimura, Kazuhiro Saeki, Teruhiko Wakayama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0070ea1813b94bfa8d89c34e8190b0a2
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id	oai:doaj.org-article:0070ea1813b94bfa8d89c34e8190b0a2
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spelling	oai:doaj.org-article:0070ea1813b94bfa8d89c34e8190b0a22021-11-18T07:28:36ZFluorescence cell imaging and manipulation using conventional halogen lamp microscopy.1932-620310.1371/journal.pone.0031638https://doaj.org/article/0070ea1813b94bfa8d89c34e8190b0a22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22347500/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy. We have developed a filter system to excite fluorescent dyes using a conventional transmission microscope equipped with a halogen lamp. This method allows us to observe previously invisible cell organelles, such as the metaphase spindle of oocytes, without causing phototoxicity. Cells remain healthy even after intensive manipulation under fluorescence observation, such as during bovine, porcine and mouse somatic cell cloning using nuclear transfer. This method does not require expensive epifluorescence equipment and so could help to reduce the science gap between developed and developing countries.Kazuo YamagataDaisaku IwamotoYukari TerashitaChong LiSayaka WakayamaYoko Hayashi-TakanakaHiroshi KimuraKazuhiro SaekiTeruhiko WakayamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31638 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kazuo Yamagata Daisaku Iwamoto Yukari Terashita Chong Li Sayaka Wakayama Yoko Hayashi-Takanaka Hiroshi Kimura Kazuhiro Saeki Teruhiko Wakayama Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
description	Technologies for vitally labeling cells with fluorescent dyes have advanced remarkably. However, to excite fluorescent dyes currently requires powerful illumination, which can cause phototoxic damage to the cells and increases the cost of microscopy. We have developed a filter system to excite fluorescent dyes using a conventional transmission microscope equipped with a halogen lamp. This method allows us to observe previously invisible cell organelles, such as the metaphase spindle of oocytes, without causing phototoxicity. Cells remain healthy even after intensive manipulation under fluorescence observation, such as during bovine, porcine and mouse somatic cell cloning using nuclear transfer. This method does not require expensive epifluorescence equipment and so could help to reduce the science gap between developed and developing countries.
format	article
author	Kazuo Yamagata Daisaku Iwamoto Yukari Terashita Chong Li Sayaka Wakayama Yoko Hayashi-Takanaka Hiroshi Kimura Kazuhiro Saeki Teruhiko Wakayama
author_facet	Kazuo Yamagata Daisaku Iwamoto Yukari Terashita Chong Li Sayaka Wakayama Yoko Hayashi-Takanaka Hiroshi Kimura Kazuhiro Saeki Teruhiko Wakayama
author_sort	Kazuo Yamagata
title	Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
title_short	Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
title_full	Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
title_fullStr	Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
title_full_unstemmed	Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
title_sort	fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0070ea1813b94bfa8d89c34e8190b0a2
work_keys_str_mv	AT kazuoyamagata fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT daisakuiwamoto fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT yukariterashita fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT chongli fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT sayakawakayama fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT yokohayashitakanaka fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT hiroshikimura fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT kazuhirosaeki fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy AT teruhikowakayama fluorescencecellimagingandmanipulationusingconventionalhalogenlampmicroscopy
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Fluorescence cell imaging and manipulation using conventional halogen lamp microscopy.

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