Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study

Abstract MicroRNA-371a-3p (miR371) has been suggested as a sensitive biomarker in testicular germ cell cancer (TGCC). We aimed to compare miR371 with the classical biomarkers α-fetoprotein (AFP) and β-human chorionic gonadotropin (hCGβ). Overall, 180 patients were prospectively enrolled in the study...

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Autores principales:	Mette Pernille Myklebust, Anna Thor, Benedikte Rosenlund, Peder Gjengstø, Ása Karlsdottir, Marianne Brydøy, Bogdan S. Bercea, Christian Olsen, Ida Johnson, Mathilde I. Berg, Carl W. Langberg, Kristine E. Andreassen, Anders Kjellman, Hege S. Haugnes, Olav Dahl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/007a32cb6b57433c9a8d6a521c3627cb
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spelling	oai:doaj.org-article:007a32cb6b57433c9a8d6a521c3627cb2021-12-02T18:49:32ZSerum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study10.1038/s41598-021-94812-22045-2322https://doaj.org/article/007a32cb6b57433c9a8d6a521c3627cb2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94812-2https://doaj.org/toc/2045-2322Abstract MicroRNA-371a-3p (miR371) has been suggested as a sensitive biomarker in testicular germ cell cancer (TGCC). We aimed to compare miR371 with the classical biomarkers α-fetoprotein (AFP) and β-human chorionic gonadotropin (hCGβ). Overall, 180 patients were prospectively enrolled in the study, with serum samples collected before and after orchiectomy. We compared the use of digital droplet PCR (RT-ddPCR) with the quantitative PCR used by others for detection of miR371. The novel RT-ddPCR protocol showed high performance in detection of miR371 in serum samples. In the study cohort, miR371 was measured using RT-ddPCR. MiR371 detected CS1 of the seminoma and the non-seminoma sub-types with a sensitivity of 87% and 89%, respectively. The total sensitivity was 89%. After orchiectomy, miR371 levels declined in 154 of 159 TGCC cases. The ratio of miR371 pre- and post-orchiectomy was 20.5 in CS1 compared to 6.5 in systemic disease. AFP and hCGβ had sensitivities of 52% and 51% in the non-seminomas. MiR371 is a sensitive marker that performs better than the classical markers in all sub-types and clinical stages. Especially for the seminomas CS1, the high sensitivity of miR371 in detecting TGCC cells may have clinical implications.Mette Pernille MyklebustAnna ThorBenedikte RosenlundPeder GjengstøÁsa KarlsdottirMarianne BrydøyBogdan S. BerceaChristian OlsenIda JohnsonMathilde I. BergCarl W. LangbergKristine E. AndreassenAnders KjellmanHege S. HaugnesOlav DahlNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mette Pernille Myklebust Anna Thor Benedikte Rosenlund Peder Gjengstø Ása Karlsdottir Marianne Brydøy Bogdan S. Bercea Christian Olsen Ida Johnson Mathilde I. Berg Carl W. Langberg Kristine E. Andreassen Anders Kjellman Hege S. Haugnes Olav Dahl Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
description	Abstract MicroRNA-371a-3p (miR371) has been suggested as a sensitive biomarker in testicular germ cell cancer (TGCC). We aimed to compare miR371 with the classical biomarkers α-fetoprotein (AFP) and β-human chorionic gonadotropin (hCGβ). Overall, 180 patients were prospectively enrolled in the study, with serum samples collected before and after orchiectomy. We compared the use of digital droplet PCR (RT-ddPCR) with the quantitative PCR used by others for detection of miR371. The novel RT-ddPCR protocol showed high performance in detection of miR371 in serum samples. In the study cohort, miR371 was measured using RT-ddPCR. MiR371 detected CS1 of the seminoma and the non-seminoma sub-types with a sensitivity of 87% and 89%, respectively. The total sensitivity was 89%. After orchiectomy, miR371 levels declined in 154 of 159 TGCC cases. The ratio of miR371 pre- and post-orchiectomy was 20.5 in CS1 compared to 6.5 in systemic disease. AFP and hCGβ had sensitivities of 52% and 51% in the non-seminomas. MiR371 is a sensitive marker that performs better than the classical markers in all sub-types and clinical stages. Especially for the seminomas CS1, the high sensitivity of miR371 in detecting TGCC cells may have clinical implications.
format	article
author	Mette Pernille Myklebust Anna Thor Benedikte Rosenlund Peder Gjengstø Ása Karlsdottir Marianne Brydøy Bogdan S. Bercea Christian Olsen Ida Johnson Mathilde I. Berg Carl W. Langberg Kristine E. Andreassen Anders Kjellman Hege S. Haugnes Olav Dahl
author_facet	Mette Pernille Myklebust Anna Thor Benedikte Rosenlund Peder Gjengstø Ása Karlsdottir Marianne Brydøy Bogdan S. Bercea Christian Olsen Ida Johnson Mathilde I. Berg Carl W. Langberg Kristine E. Andreassen Anders Kjellman Hege S. Haugnes Olav Dahl
author_sort	Mette Pernille Myklebust
title	Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
title_short	Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
title_full	Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
title_fullStr	Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
title_full_unstemmed	Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
title_sort	serum mir371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet pcr in a prospective study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/007a32cb6b57433c9a8d6a521c3627cb
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Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study

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