Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.

The magnocellular neurons (MCNs) in the hypothalamus selectively express either oxytocin (OXT) or vasopressin (AVP) neuropeptide genes, a property that defines their phenotypes. Here we examine the molecular basis of this selectivity in the OXT MCNs by stereotaxic microinjections of adeno-associated...

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Autores principales:	Raymond L Fields, Todd A Ponzio, Makoto Kawasaki, Harold Gainer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/007a3c0d7667495c99002bf301bddbd0
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spelling	oai:doaj.org-article:007a3c0d7667495c99002bf301bddbd02021-11-18T07:27:12ZCell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.1932-620310.1371/journal.pone.0032085https://doaj.org/article/007a3c0d7667495c99002bf301bddbd02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22363799/?tool=EBIhttps://doaj.org/toc/1932-6203The magnocellular neurons (MCNs) in the hypothalamus selectively express either oxytocin (OXT) or vasopressin (AVP) neuropeptide genes, a property that defines their phenotypes. Here we examine the molecular basis of this selectivity in the OXT MCNs by stereotaxic microinjections of adeno-associated virus (AAV) vectors that contain various OXT gene promoter deletion constructs using EGFP as the reporter into the rat supraoptic nucleus (SON). Two weeks following injection of the AAVs, immunohistochemical assays of EGFP expression from these constructs were done to determine whether the EGFP reporter co-localizes with either the OXT- or AVP-immunoreactivity in the MCNs. The results show that the key elements in the OT gene promoter that regulate the cell-type specific expression the SON are located -216 to -100 bp upstream of the transcription start site. We hypothesize that within this 116 bp domain a repressor exists that inhibits expression specifically in AVP MCNs, thereby leading to the cell-type specific expression of the OXT gene only in the OXT MCNs.Raymond L FieldsTodd A PonzioMakoto KawasakiHarold GainerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e32085 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raymond L Fields Todd A Ponzio Makoto Kawasaki Harold Gainer Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
description	The magnocellular neurons (MCNs) in the hypothalamus selectively express either oxytocin (OXT) or vasopressin (AVP) neuropeptide genes, a property that defines their phenotypes. Here we examine the molecular basis of this selectivity in the OXT MCNs by stereotaxic microinjections of adeno-associated virus (AAV) vectors that contain various OXT gene promoter deletion constructs using EGFP as the reporter into the rat supraoptic nucleus (SON). Two weeks following injection of the AAVs, immunohistochemical assays of EGFP expression from these constructs were done to determine whether the EGFP reporter co-localizes with either the OXT- or AVP-immunoreactivity in the MCNs. The results show that the key elements in the OT gene promoter that regulate the cell-type specific expression the SON are located -216 to -100 bp upstream of the transcription start site. We hypothesize that within this 116 bp domain a repressor exists that inhibits expression specifically in AVP MCNs, thereby leading to the cell-type specific expression of the OXT gene only in the OXT MCNs.
format	article
author	Raymond L Fields Todd A Ponzio Makoto Kawasaki Harold Gainer
author_facet	Raymond L Fields Todd A Ponzio Makoto Kawasaki Harold Gainer
author_sort	Raymond L Fields
title	Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
title_short	Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
title_full	Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
title_fullStr	Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
title_full_unstemmed	Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
title_sort	cell-type specific oxytocin gene expression from aav delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/007a3c0d7667495c99002bf301bddbd0
work_keys_str_mv	AT raymondlfields celltypespecificoxytocingeneexpressionfromaavdeliveredpromoterdeletionconstructsintotheratsupraopticnucleusinvivo AT toddaponzio celltypespecificoxytocingeneexpressionfromaavdeliveredpromoterdeletionconstructsintotheratsupraopticnucleusinvivo AT makotokawasaki celltypespecificoxytocingeneexpressionfromaavdeliveredpromoterdeletionconstructsintotheratsupraopticnucleusinvivo AT haroldgainer celltypespecificoxytocingeneexpressionfromaavdeliveredpromoterdeletionconstructsintotheratsupraopticnucleusinvivo
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Cell-type specific oxytocin gene expression from AAV delivered promoter deletion constructs into the rat supraoptic nucleus in vivo.

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