Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice

Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice

Abstract The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study...

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Autores principales: Raife Dilek Turan, Cihan Tastan, Derya Dilek Kancagi, Bulut Yurtsever, Gozde Sir Karakus, Samed Ozer, Selen Abanuz, Didem Cakirsoy, Gamze Tumentemur, Sevda Demir, Utku Seyis, Recai Kuzay, Muhammer Elek, Miyase Ezgi Kocaoglu, Gurcan Ertop, Serap Arbak, Merve Acikel Elmas, Cansu Hemsinlioglu, Ozden Hatirnaz Ng, Sezer Akyoney, Ilayda Sahin, Cavit Kerem Kayhan, Fatma Tokat, Gurler Akpinar, Murat Kasap, Ayse Sesin Kocagoz, Ugur Ozbek, Dilek Telci, Fikrettin Sahin, Koray Yalcin, Siret Ratip, Umit Ince, Ercument Ovali
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/0080510c27ab4fa68a13783ab005d11d
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spelling oai:doaj.org-article:0080510c27ab4fa68a13783ab005d11d2021-12-02T16:35:28ZGamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice10.1038/s41598-021-95086-42045-2322https://doaj.org/article/0080510c27ab4fa68a13783ab005d11d2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95086-4https://doaj.org/toc/2045-2322Abstract The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 1013 or 1014 viral RNA copy per dose) of OZG-38.61.3 was initially determined in BALB/c mice. This was followed by testing the immunogenicity and protective efficacy of the vaccine. Human ACE2-encoding transgenic mice were immunized and then infected with the SARS-CoV-2 virus for the challenge test. This study shows that vaccinated mice have lowered SARS-CoV-2 viral RNA copy numbers both in oropharyngeal specimens and in the histological analysis of the lung tissues along with humoral and cellular immune responses, including the neutralizing antibodies similar to those shown in BALB/c mice without substantial toxicity. Subsequently, plans are being made for the commencement of Phase 1 clinical trial of the OZG-38.61.3 vaccine for the COVID-19 pandemic.Raife Dilek TuranCihan TastanDerya Dilek KancagiBulut YurtseverGozde Sir KarakusSamed OzerSelen AbanuzDidem CakirsoyGamze TumentemurSevda DemirUtku SeyisRecai KuzayMuhammer ElekMiyase Ezgi KocaogluGurcan ErtopSerap ArbakMerve Acikel ElmasCansu HemsinliogluOzden Hatirnaz NgSezer AkyoneyIlayda SahinCavit Kerem KayhanFatma TokatGurler AkpinarMurat KasapAyse Sesin KocagozUgur OzbekDilek TelciFikrettin SahinKoray YalcinSiret RatipUmit InceErcument OvaliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Raife Dilek Turan
Cihan Tastan
Derya Dilek Kancagi
Bulut Yurtsever
Gozde Sir Karakus
Samed Ozer
Selen Abanuz
Didem Cakirsoy
Gamze Tumentemur
Sevda Demir
Utku Seyis
Recai Kuzay
Muhammer Elek
Miyase Ezgi Kocaoglu
Gurcan Ertop
Serap Arbak
Merve Acikel Elmas
Cansu Hemsinlioglu
Ozden Hatirnaz Ng
Sezer Akyoney
Ilayda Sahin
Cavit Kerem Kayhan
Fatma Tokat
Gurler Akpinar
Murat Kasap
Ayse Sesin Kocagoz
Ugur Ozbek
Dilek Telci
Fikrettin Sahin
Koray Yalcin
Siret Ratip
Umit Ince
Ercument Ovali
Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
description Abstract The SARS-CoV-2 virus caused the most severe pandemic around the world, and vaccine development for urgent use became a crucial issue. Inactivated virus formulated vaccines such as Hepatitis A and smallpox proved to be reliable approaches for immunization for prolonged periods. In this study, a gamma-irradiated inactivated virus vaccine does not require an extra purification process, unlike the chemically inactivated vaccines. Hence, the novelty of our vaccine candidate (OZG-38.61.3) is that it is a non-adjuvant added, gamma-irradiated, and intradermally applied inactive viral vaccine. Efficiency and safety dose (either 1013 or 1014 viral RNA copy per dose) of OZG-38.61.3 was initially determined in BALB/c mice. This was followed by testing the immunogenicity and protective efficacy of the vaccine. Human ACE2-encoding transgenic mice were immunized and then infected with the SARS-CoV-2 virus for the challenge test. This study shows that vaccinated mice have lowered SARS-CoV-2 viral RNA copy numbers both in oropharyngeal specimens and in the histological analysis of the lung tissues along with humoral and cellular immune responses, including the neutralizing antibodies similar to those shown in BALB/c mice without substantial toxicity. Subsequently, plans are being made for the commencement of Phase 1 clinical trial of the OZG-38.61.3 vaccine for the COVID-19 pandemic.
format article
author Raife Dilek Turan
Cihan Tastan
Derya Dilek Kancagi
Bulut Yurtsever
Gozde Sir Karakus
Samed Ozer
Selen Abanuz
Didem Cakirsoy
Gamze Tumentemur
Sevda Demir
Utku Seyis
Recai Kuzay
Muhammer Elek
Miyase Ezgi Kocaoglu
Gurcan Ertop
Serap Arbak
Merve Acikel Elmas
Cansu Hemsinlioglu
Ozden Hatirnaz Ng
Sezer Akyoney
Ilayda Sahin
Cavit Kerem Kayhan
Fatma Tokat
Gurler Akpinar
Murat Kasap
Ayse Sesin Kocagoz
Ugur Ozbek
Dilek Telci
Fikrettin Sahin
Koray Yalcin
Siret Ratip
Umit Ince
Ercument Ovali
author_facet Raife Dilek Turan
Cihan Tastan
Derya Dilek Kancagi
Bulut Yurtsever
Gozde Sir Karakus
Samed Ozer
Selen Abanuz
Didem Cakirsoy
Gamze Tumentemur
Sevda Demir
Utku Seyis
Recai Kuzay
Muhammer Elek
Miyase Ezgi Kocaoglu
Gurcan Ertop
Serap Arbak
Merve Acikel Elmas
Cansu Hemsinlioglu
Ozden Hatirnaz Ng
Sezer Akyoney
Ilayda Sahin
Cavit Kerem Kayhan
Fatma Tokat
Gurler Akpinar
Murat Kasap
Ayse Sesin Kocagoz
Ugur Ozbek
Dilek Telci
Fikrettin Sahin
Koray Yalcin
Siret Ratip
Umit Ince
Ercument Ovali
author_sort Raife Dilek Turan
title Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
title_short Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
title_full Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
title_fullStr Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
title_full_unstemmed Gamma-irradiated SARS-CoV-2 vaccine candidate, OZG-38.61.3, confers protection from SARS-CoV-2 challenge in human ACEII-transgenic mice
title_sort gamma-irradiated sars-cov-2 vaccine candidate, ozg-38.61.3, confers protection from sars-cov-2 challenge in human aceii-transgenic mice
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/0080510c27ab4fa68a13783ab005d11d
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