Combining 3D single molecule localization strategies for reproducible bioimaging

3D single molecule localization microscopy suffers from several experimental biases that degrade the resolution or localization precision. Here the authors present a dual-view detection scheme combining supercritical angle fluorescence and astigmatic imaging to obtain precise and unbiased 3D super r...

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Autores principales:	Clément Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Christophe Leterrier, Aurélie Baron, Marie-Ange Badet-Denisot, Boris Vauzeilles, Emmanuel Fort, Sandrine Lévêque-Fort
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/0084b856eb874184ac663769fdb8462a
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spelling	oai:doaj.org-article:0084b856eb874184ac663769fdb8462a2021-12-02T17:32:10ZCombining 3D single molecule localization strategies for reproducible bioimaging10.1038/s41467-019-09901-82041-1723https://doaj.org/article/0084b856eb874184ac663769fdb8462a2019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09901-8https://doaj.org/toc/2041-17233D single molecule localization microscopy suffers from several experimental biases that degrade the resolution or localization precision. Here the authors present a dual-view detection scheme combining supercritical angle fluorescence and astigmatic imaging to obtain precise and unbiased 3D super resolution images.Clément CabrielNicolas BourgPierre JouchetGuillaume DupuisChristophe LeterrierAurélie BaronMarie-Ange Badet-DenisotBoris VauzeillesEmmanuel FortSandrine Lévêque-FortNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Clément Cabriel Nicolas Bourg Pierre Jouchet Guillaume Dupuis Christophe Leterrier Aurélie Baron Marie-Ange Badet-Denisot Boris Vauzeilles Emmanuel Fort Sandrine Lévêque-Fort Combining 3D single molecule localization strategies for reproducible bioimaging
description	3D single molecule localization microscopy suffers from several experimental biases that degrade the resolution or localization precision. Here the authors present a dual-view detection scheme combining supercritical angle fluorescence and astigmatic imaging to obtain precise and unbiased 3D super resolution images.
format	article
author	Clément Cabriel Nicolas Bourg Pierre Jouchet Guillaume Dupuis Christophe Leterrier Aurélie Baron Marie-Ange Badet-Denisot Boris Vauzeilles Emmanuel Fort Sandrine Lévêque-Fort
author_facet	Clément Cabriel Nicolas Bourg Pierre Jouchet Guillaume Dupuis Christophe Leterrier Aurélie Baron Marie-Ange Badet-Denisot Boris Vauzeilles Emmanuel Fort Sandrine Lévêque-Fort
author_sort	Clément Cabriel
title	Combining 3D single molecule localization strategies for reproducible bioimaging
title_short	Combining 3D single molecule localization strategies for reproducible bioimaging
title_full	Combining 3D single molecule localization strategies for reproducible bioimaging
title_fullStr	Combining 3D single molecule localization strategies for reproducible bioimaging
title_full_unstemmed	Combining 3D single molecule localization strategies for reproducible bioimaging
title_sort	combining 3d single molecule localization strategies for reproducible bioimaging
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/0084b856eb874184ac663769fdb8462a
work_keys_str_mv	AT clementcabriel combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT nicolasbourg combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT pierrejouchet combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT guillaumedupuis combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT christopheleterrier combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT aureliebaron combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT marieangebadetdenisot combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT borisvauzeilles combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT emmanuelfort combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging AT sandrinelevequefort combining3dsinglemoleculelocalizationstrategiesforreproduciblebioimaging
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Combining 3D single molecule localization strategies for reproducible bioimaging

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