Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines

Water quality deterioration in source waters poses increased health, environmental, and economic risks. Here, we genotyped Cryptosporidium spp. obtained from water samples of Laguna Lake, Philippines, and its tributaries for the purpose of source-tracking fecal contamination. A total of 104 surface...

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Autores principales:	Laurice Beatrice Raphaelle O. dela Peña, Mark Raymond A. Vejano, Windell L. Rivera
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	cryptosporidium laguna lake microbial source tracking philippines surface water Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/0089eb3e441c4d20867665a3824514b3
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spelling	oai:doaj.org-article:0089eb3e441c4d20867665a3824514b32021-11-06T06:05:23ZMolecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines1477-89201996-782910.2166/wh.2021.059https://doaj.org/article/0089eb3e441c4d20867665a3824514b32021-06-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/3/534https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Water quality deterioration in source waters poses increased health, environmental, and economic risks. Here, we genotyped Cryptosporidium spp. obtained from water samples of Laguna Lake, Philippines, and its tributaries for the purpose of source-tracking fecal contamination. A total of 104 surface water samples were collected over a 1-year period (March 2018 to April 2019). Detection of Cryptosporidium was carried out using genus-specific primers targeting a fragment of the small subunit (SSU) rRNA gene. The study revealed 8 (14%) tributary samples and 1 (2.77%) lake sample positive for contamination. The species were determined to be C. parvum (n = 4), C. muris (n = 2), C. hominis (n = 1), C. galli (n = 1), C. baileyi (n = 1), C. suis (n = 1), as well as rat genotype IV (n = 1). Two species were detected in duck (C. baileyi) and cattle (C. parvum) fecal samples. The data presented suggest that Cryptosporidium contamination is likely to come from sewage or human feces as well as various agricultural sources (i.e. cattle, swine, and poultry). This information reveals the importance of mitigating fecal pollution in the lake system and minimizing health risks due to exposure to zoonotic Cryptosporidium species. HIGHLIGHTS Zoonotic and pathogenic species of Cryptosporidium in a freshwater reservoir point to domestic and agricultural sources of fecal contamination.; Cryptosporidium can be a potential marker for microbial source tracking in the Laguna Lake watershed to augment current monitoring efforts.;Laurice Beatrice Raphaelle O. dela PeñaMark Raymond A. VejanoWindell L. RiveraIWA Publishingarticlecryptosporidiumlaguna lakemicrobial source trackingphilippinessurface waterPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 3, Pp 534-544 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryptosporidium laguna lake microbial source tracking philippines surface water Public aspects of medicine RA1-1270
spellingShingle	cryptosporidium laguna lake microbial source tracking philippines surface water Public aspects of medicine RA1-1270 Laurice Beatrice Raphaelle O. dela Peña Mark Raymond A. Vejano Windell L. Rivera Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
description	Water quality deterioration in source waters poses increased health, environmental, and economic risks. Here, we genotyped Cryptosporidium spp. obtained from water samples of Laguna Lake, Philippines, and its tributaries for the purpose of source-tracking fecal contamination. A total of 104 surface water samples were collected over a 1-year period (March 2018 to April 2019). Detection of Cryptosporidium was carried out using genus-specific primers targeting a fragment of the small subunit (SSU) rRNA gene. The study revealed 8 (14%) tributary samples and 1 (2.77%) lake sample positive for contamination. The species were determined to be C. parvum (n = 4), C. muris (n = 2), C. hominis (n = 1), C. galli (n = 1), C. baileyi (n = 1), C. suis (n = 1), as well as rat genotype IV (n = 1). Two species were detected in duck (C. baileyi) and cattle (C. parvum) fecal samples. The data presented suggest that Cryptosporidium contamination is likely to come from sewage or human feces as well as various agricultural sources (i.e. cattle, swine, and poultry). This information reveals the importance of mitigating fecal pollution in the lake system and minimizing health risks due to exposure to zoonotic Cryptosporidium species. HIGHLIGHTS Zoonotic and pathogenic species of Cryptosporidium in a freshwater reservoir point to domestic and agricultural sources of fecal contamination.; Cryptosporidium can be a potential marker for microbial source tracking in the Laguna Lake watershed to augment current monitoring efforts.;
format	article
author	Laurice Beatrice Raphaelle O. dela Peña Mark Raymond A. Vejano Windell L. Rivera
author_facet	Laurice Beatrice Raphaelle O. dela Peña Mark Raymond A. Vejano Windell L. Rivera
author_sort	Laurice Beatrice Raphaelle O. dela Peña
title	Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
title_short	Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
title_full	Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
title_fullStr	Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
title_full_unstemmed	Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines
title_sort	molecular surveillance of cryptosporidium spp. for microbial source tracking of fecal contamination in laguna lake, philippines
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/0089eb3e441c4d20867665a3824514b3
work_keys_str_mv	AT lauricebeatriceraphaelleodelapena molecularsurveillanceofcryptosporidiumsppformicrobialsourcetrackingoffecalcontaminationinlagunalakephilippines AT markraymondavejano molecularsurveillanceofcryptosporidiumsppformicrobialsourcetrackingoffecalcontaminationinlagunalakephilippines AT windelllrivera molecularsurveillanceofcryptosporidiumsppformicrobialsourcetrackingoffecalcontaminationinlagunalakephilippines
_version_	1718443834681065472

Molecular surveillance of Cryptosporidium spp. for microbial source tracking of fecal contamination in Laguna Lake, Philippines

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