Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress

Saba Naqvi1, Mohammad Samim2, MZ Abdin3, Farhan Jalees Ahmed4, AN Maitra5, CK Prashant6, Amit K Dinda61Faculty of Engineering and Interdisciplinary Sciences, 2Department of Chemistry, 3Department of Biotechnology, Faculty of Science, 4Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard,...

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Autores principales:	Saba Naqvi, Mohammad Samim, MZ Abdin, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/008a9c3ec68744f189b17c58418b97db
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spelling	oai:doaj.org-article:008a9c3ec68744f189b17c58418b97db2021-12-02T07:45:07ZConcentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress1176-91141178-2013https://doaj.org/article/008a9c3ec68744f189b17c58418b97db2010-11-01T00:00:00Zhttp://www.dovepress.com/concentration-dependent-toxicity-of-iron-oxide-nanoparticles-mediated--a5655https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Saba Naqvi1, Mohammad Samim2, MZ Abdin3, Farhan Jalees Ahmed4, AN Maitra5, CK Prashant6, Amit K Dinda61Faculty of Engineering and Interdisciplinary Sciences, 2Department of Chemistry, 3Department of Biotechnology, Faculty of Science, 4Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, 5Department of Chemistry, University of Delhi, 6Department of Pathology, All India Institute of Medical Sciences, New Delhi, IndiaAbstract: Iron oxide nanoparticles with unique magnetic properties have a high potential for use in several biomedical, bioengineering and in vivo applications, including tissue repair, magnetic resonance imaging, immunoassay, drug delivery, detoxification of biologic fluids, cell sorting, and hyperthermia. Although various surface modifications are being done for making these nonbiodegradable nanoparticles more biocompatible, their toxic potential is still a major concern. The current in vitro study of the interaction of superparamagnetic iron oxide nanoparticles of mean diameter 30 nm coated with Tween 80 and murine macrophage (J774) cells was undertaken to evaluate the dose- and time-dependent toxic potential, as well as investigate the role of oxidative stress in the toxicity. A 15&ndash;30 nm size range of spherical nanoparticles were characterized by transmission electron microscopy and zeta sizer. MTT assay showed &gt;95% viability of cells in lower concentrations (25&ndash;200 &micro;g/mL) and up to three hours of exposure, whereas at higher concentrations (300&ndash;500 &micro;g/mL) and prolonged (six hours) exposure viability reduced to 55%&ndash;65%. Necrosis-apoptosis assay by propidium iodide and Hoechst-33342 staining revealed loss of the majority of the cells by apoptosis. H2DCFDDA assay to quantify generation of intracellular reactive oxygen species (ROS) indicated that exposure to a higher concentration of nanoparticles resulted in enhanced ROS generation, leading to cell injury and death. The cell membrane injury induced by nanoparticles studied using the lactate dehydrogenase assay, showed both concentration- and time-dependent damage. Thus, this study concluded that use of a low optimum concentration of superparamagnetic iron oxide nanoparticles is important for avoidance of oxidative stress-induced cell injury and death.Keywords: superparamagnetic iron oxide nanoparticles, cytotoxicity, MTT assay, J774 cell line Saba NaqviMohammad SamimMZ Abdinet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 983-989 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Saba Naqvi Mohammad Samim MZ Abdin et al Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
description	Saba Naqvi1, Mohammad Samim2, MZ Abdin3, Farhan Jalees Ahmed4, AN Maitra5, CK Prashant6, Amit K Dinda61Faculty of Engineering and Interdisciplinary Sciences, 2Department of Chemistry, 3Department of Biotechnology, Faculty of Science, 4Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, Hamdard University, 5Department of Chemistry, University of Delhi, 6Department of Pathology, All India Institute of Medical Sciences, New Delhi, IndiaAbstract: Iron oxide nanoparticles with unique magnetic properties have a high potential for use in several biomedical, bioengineering and in vivo applications, including tissue repair, magnetic resonance imaging, immunoassay, drug delivery, detoxification of biologic fluids, cell sorting, and hyperthermia. Although various surface modifications are being done for making these nonbiodegradable nanoparticles more biocompatible, their toxic potential is still a major concern. The current in vitro study of the interaction of superparamagnetic iron oxide nanoparticles of mean diameter 30 nm coated with Tween 80 and murine macrophage (J774) cells was undertaken to evaluate the dose- and time-dependent toxic potential, as well as investigate the role of oxidative stress in the toxicity. A 15&ndash;30 nm size range of spherical nanoparticles were characterized by transmission electron microscopy and zeta sizer. MTT assay showed &gt;95% viability of cells in lower concentrations (25&ndash;200 &micro;g/mL) and up to three hours of exposure, whereas at higher concentrations (300&ndash;500 &micro;g/mL) and prolonged (six hours) exposure viability reduced to 55%&ndash;65%. Necrosis-apoptosis assay by propidium iodide and Hoechst-33342 staining revealed loss of the majority of the cells by apoptosis. H2DCFDDA assay to quantify generation of intracellular reactive oxygen species (ROS) indicated that exposure to a higher concentration of nanoparticles resulted in enhanced ROS generation, leading to cell injury and death. The cell membrane injury induced by nanoparticles studied using the lactate dehydrogenase assay, showed both concentration- and time-dependent damage. Thus, this study concluded that use of a low optimum concentration of superparamagnetic iron oxide nanoparticles is important for avoidance of oxidative stress-induced cell injury and death.Keywords: superparamagnetic iron oxide nanoparticles, cytotoxicity, MTT assay, J774 cell line
format	article
author	Saba Naqvi Mohammad Samim MZ Abdin et al
author_facet	Saba Naqvi Mohammad Samim MZ Abdin et al
author_sort	Saba Naqvi
title	Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
title_short	Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
title_full	Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
title_fullStr	Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
title_full_unstemmed	Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
title_sort	concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/008a9c3ec68744f189b17c58418b97db
work_keys_str_mv	AT sabanaqvi concentrationdependenttoxicityofironoxidenanoparticlesmediatedbyincreasedoxidativestress AT mohammadsamim concentrationdependenttoxicityofironoxidenanoparticlesmediatedbyincreasedoxidativestress AT mzabdin concentrationdependenttoxicityofironoxidenanoparticlesmediatedbyincreasedoxidativestress AT etal concentrationdependenttoxicityofironoxidenanoparticlesmediatedbyincreasedoxidativestress
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Concentration-dependent toxicity of iron oxide nanoparticles mediated by increased oxidative stress

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