NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress
Abstract Doxorubicin (DOX) is an anthracycline antibiotic used in the clinical treatment of cancer, but its use is limited due to its cardiotoxic effects. Therefore, it is necessary to explore natural compounds that are effective in protecting against the cardiotoxicity caused by DOX. Neutral Morche...
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Wiley
2021
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oai:doaj.org-article:008dfff0966e4904aef051697cb27e2c2021-11-04T13:06:43ZNMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress2048-717710.1002/fsn3.2586https://doaj.org/article/008dfff0966e4904aef051697cb27e2c2021-11-01T00:00:00Zhttps://doi.org/10.1002/fsn3.2586https://doaj.org/toc/2048-7177Abstract Doxorubicin (DOX) is an anthracycline antibiotic used in the clinical treatment of cancer, but its use is limited due to its cardiotoxic effects. Therefore, it is necessary to explore natural compounds that are effective in protecting against the cardiotoxicity caused by DOX. Neutral Morchella conica polysaccharides‐2 (NMCP‐2) is a natural polysaccharide with antioxidant activity that was isolated and purified from Morchella conica in our laboratory's previous study. This study aimed to investigate the possible protective effect of NMCP‐2 on DOX‐induced cardiotoxicity and the potential underlying mechanisms. The model of DOX‐induced H9C2 cells and the model of DOX‐induced mice were used in this study. In in vitro studies of H9C2 myocardial cells, NMCP‐2 effectively increased the activity of H9C2 cells, reducing the levels of lactate dehydrogenase (LDH). In the mouse model of DOX‐induced chronic cardiotoxicity, NMCP‐2 significantly reduced the cardiac index, reduced the release of serum cardiac enzymes, and improved the pathology of murine myocardial tissues, thereby alleviating DOX‐induced cardiotoxicity. Further mechanism studies showed that pretreatment with NMCP‐2 counteracted the oxidative stress induced by DOX, as indicated by increasing superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) activities, and malondialdehyde (MDA) production decreased. In addition, we observed NMCP‐2 inhibited the activation of the mitochondrial apoptosis pathway and regulated the disordered expression of Bcl‐2 and Bax in the myocardial tissues of DOX‐treated mice. These findings indicated that NMCP‐2, a natural bioactive compound, could potentially be used as a food supplement to reduce the cardiotoxicity caused by DOX.Na XuYi LuXinmiao YaoRui ZhaoZhebin LiJialei LiYinglei ZhangBo LiYe ZhouHuifang ShenLiqun WangKaixin ChenLi YangShuwen LuWileyarticlecardiotoxicitydoxorubicinmitochondrial apoptosisNMCP‐2oxidative stressNutrition. Foods and food supplyTX341-641ENFood Science & Nutrition, Vol 9, Iss 11, Pp 6262-6273 (2021) |
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| collection |
DOAJ |
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cardiotoxicity doxorubicin mitochondrial apoptosis NMCP‐2 oxidative stress Nutrition. Foods and food supply TX341-641 |
| spellingShingle |
cardiotoxicity doxorubicin mitochondrial apoptosis NMCP‐2 oxidative stress Nutrition. Foods and food supply TX341-641 Na Xu Yi Lu Xinmiao Yao Rui Zhao Zhebin Li Jialei Li Yinglei Zhang Bo Li Ye Zhou Huifang Shen Liqun Wang Kaixin Chen Li Yang Shuwen Lu NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| description |
Abstract Doxorubicin (DOX) is an anthracycline antibiotic used in the clinical treatment of cancer, but its use is limited due to its cardiotoxic effects. Therefore, it is necessary to explore natural compounds that are effective in protecting against the cardiotoxicity caused by DOX. Neutral Morchella conica polysaccharides‐2 (NMCP‐2) is a natural polysaccharide with antioxidant activity that was isolated and purified from Morchella conica in our laboratory's previous study. This study aimed to investigate the possible protective effect of NMCP‐2 on DOX‐induced cardiotoxicity and the potential underlying mechanisms. The model of DOX‐induced H9C2 cells and the model of DOX‐induced mice were used in this study. In in vitro studies of H9C2 myocardial cells, NMCP‐2 effectively increased the activity of H9C2 cells, reducing the levels of lactate dehydrogenase (LDH). In the mouse model of DOX‐induced chronic cardiotoxicity, NMCP‐2 significantly reduced the cardiac index, reduced the release of serum cardiac enzymes, and improved the pathology of murine myocardial tissues, thereby alleviating DOX‐induced cardiotoxicity. Further mechanism studies showed that pretreatment with NMCP‐2 counteracted the oxidative stress induced by DOX, as indicated by increasing superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) activities, and malondialdehyde (MDA) production decreased. In addition, we observed NMCP‐2 inhibited the activation of the mitochondrial apoptosis pathway and regulated the disordered expression of Bcl‐2 and Bax in the myocardial tissues of DOX‐treated mice. These findings indicated that NMCP‐2, a natural bioactive compound, could potentially be used as a food supplement to reduce the cardiotoxicity caused by DOX. |
| format |
article |
| author |
Na Xu Yi Lu Xinmiao Yao Rui Zhao Zhebin Li Jialei Li Yinglei Zhang Bo Li Ye Zhou Huifang Shen Liqun Wang Kaixin Chen Li Yang Shuwen Lu |
| author_facet |
Na Xu Yi Lu Xinmiao Yao Rui Zhao Zhebin Li Jialei Li Yinglei Zhang Bo Li Ye Zhou Huifang Shen Liqun Wang Kaixin Chen Li Yang Shuwen Lu |
| author_sort |
Na Xu |
| title |
NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| title_short |
NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| title_full |
NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| title_fullStr |
NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| title_full_unstemmed |
NMCP‐2 polysaccharide purified from Morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| title_sort |
nmcp‐2 polysaccharide purified from morchella conica effectively prevents doxorubicin‐induced cardiotoxicity by decreasing cardiomyocyte apoptosis and myocardial oxidative stress |
| publisher |
Wiley |
| publishDate |
2021 |
| url |
https://doaj.org/article/008dfff0966e4904aef051697cb27e2c |
| work_keys_str_mv |
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