Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence

Abstract. Nopriawansyah N, Rauf A, Kusumah YM, Nurmansyah A, Koesmaryono Y. 2019. Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence. Biodiversitas 20: 2685-2692. Cassava me...

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Autores principales:	Nopriawansyah Nopriawansyah, AUNU RAUF, YAYI M. KUSUMAH, ALI NURMANSYAH, YONNY KOESMARYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cassava mealybug , co1, genetic diversity, phenacoccus manihoti Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0093ebd30dcb47d6b5bd8cdb24112e76
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spelling	oai:doaj.org-article:0093ebd30dcb47d6b5bd8cdb24112e762021-11-21T21:54:25ZGenetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence1412-033X2085-472210.13057/biodiv/d200933https://doaj.org/article/0093ebd30dcb47d6b5bd8cdb24112e762019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3912https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nopriawansyah N, Rauf A, Kusumah YM, Nurmansyah A, Koesmaryono Y. 2019. Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence. Biodiversitas 20: 2685-2692. Cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) is an invasive pest native to South America. Research was conducted with the objective to study genetic variation of cassava mealybug from different geographic areas in Indonesia. Thirty-six samples of cassava mealybugs were collected from eight different population locations. Genomic DNA was extracted from individual specimens of P. manihoti using gSYNC DNA Extraction Kit from Geneaid Lot No. FB221707. PCR amplification using primary CO1 gene showed the target position as long as 518 bp. Analysis of homology from eight populations revealed eight haplotypes in Indonesia with different variable sites. Genetic distance analysis was done by calculating base pair differences in each population. The percentage of genetic distance between populations was 0.0000% -0.00409%. The results of the analyst using the Kimura-2 parameter showed that the population was divided into 2 clusters. The highest haplotype (Hd) diversity value was 0.8333, while the lowest was zero (Hd = 0). The highest nucleotide diversity (?) was 0.00225 and the lowest was 0.000000. The genetic distance and nucleotide diversity were low because P. manihoti was thelytoky parthenogenesis.Nopriawansyah NopriawansyahAUNU RAUFYAYI M. KUSUMAHALI NURMANSYAHYONNY KOESMARYONOMBI & UNS Soloarticlecassava mealybug , co1, genetic diversity, phenacoccus manihotiBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cassava mealybug , co1, genetic diversity, phenacoccus manihoti Biology (General) QH301-705.5
spellingShingle	cassava mealybug , co1, genetic diversity, phenacoccus manihoti Biology (General) QH301-705.5 Nopriawansyah Nopriawansyah AUNU RAUF YAYI M. KUSUMAH ALI NURMANSYAH YONNY KOESMARYONO Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
description	Abstract. Nopriawansyah N, Rauf A, Kusumah YM, Nurmansyah A, Koesmaryono Y. 2019. Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence. Biodiversitas 20: 2685-2692. Cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) is an invasive pest native to South America. Research was conducted with the objective to study genetic variation of cassava mealybug from different geographic areas in Indonesia. Thirty-six samples of cassava mealybugs were collected from eight different population locations. Genomic DNA was extracted from individual specimens of P. manihoti using gSYNC DNA Extraction Kit from Geneaid Lot No. FB221707. PCR amplification using primary CO1 gene showed the target position as long as 518 bp. Analysis of homology from eight populations revealed eight haplotypes in Indonesia with different variable sites. Genetic distance analysis was done by calculating base pair differences in each population. The percentage of genetic distance between populations was 0.0000% -0.00409%. The results of the analyst using the Kimura-2 parameter showed that the population was divided into 2 clusters. The highest haplotype (Hd) diversity value was 0.8333, while the lowest was zero (Hd = 0). The highest nucleotide diversity (?) was 0.00225 and the lowest was 0.000000. The genetic distance and nucleotide diversity were low because P. manihoti was thelytoky parthenogenesis.
format	article
author	Nopriawansyah Nopriawansyah AUNU RAUF YAYI M. KUSUMAH ALI NURMANSYAH YONNY KOESMARYONO
author_facet	Nopriawansyah Nopriawansyah AUNU RAUF YAYI M. KUSUMAH ALI NURMANSYAH YONNY KOESMARYONO
author_sort	Nopriawansyah Nopriawansyah
title	Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
title_short	Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
title_full	Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
title_fullStr	Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
title_full_unstemmed	Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence
title_sort	genetic variation among the geographic population of cassava mealybug phenacoccus manihoti (hemiptera: pseudococcidae) in indonesia inferred from mitochondrial coi gene sequence
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/0093ebd30dcb47d6b5bd8cdb24112e76
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Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence

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