High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.

Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pierre Philippe Mbehang Nguema, Richard Onanga, Guy Roger Ndong Atome, Jean Jules Tewa, Arsène Mabika Mabika, Jean Ulrich Muandze Nzambe, Jean Constant Obague Mbeang, Paul Yannick Bitome Essono, François Bretagnolle, Sylvain Godreuil
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/009751dbd6504701b48bcd8cba2e6f44
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:009751dbd6504701b48bcd8cba2e6f44
record_format	dspace
spelling	oai:doaj.org-article:009751dbd6504701b48bcd8cba2e6f442021-12-02T20:07:55ZHigh level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.1932-620310.1371/journal.pone.0257994https://doaj.org/article/009751dbd6504701b48bcd8cba2e6f442021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257994https://doaj.org/toc/1932-6203Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance.Pierre Philippe Mbehang NguemaRichard OnangaGuy Roger Ndong AtomeJean Jules TewaArsène Mabika MabikaJean Ulrich Muandze NzambeJean Constant Obague MbeangPaul Yannick Bitome EssonoFrançois BretagnolleSylvain GodreuilPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257994 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pierre Philippe Mbehang Nguema Richard Onanga Guy Roger Ndong Atome Jean Jules Tewa Arsène Mabika Mabika Jean Ulrich Muandze Nzambe Jean Constant Obague Mbeang Paul Yannick Bitome Essono François Bretagnolle Sylvain Godreuil High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
description	Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines. The preliminary test for ESBL-producing Enterobacteriaceae was performed by replicating enterobacterial colonies on MacConkey agar supplemented with 2 mg/L cefotaxime (MCA+CTX). Extended-spectrum beta-lactamase (ESBL) production was confirmed with the double-disc synergy test (DDST). The inhibition zone diameters were read with SirScan. Among the 130 bacterial colonies isolated from 125 fecal samples, 90 enterobacterial isolates were identified. Escherichia coli (61%) was the most prevalent, followed by Enterobacter cloacae (8%), Proteus mirabilis (8%), Klebsiella variicola (7%), Klebsiella aerogenes (7%), Klebsiella oxytoca (4%), Citrobacter freundii (3%), Klebsiella pneumoniae (1%) and Serratia marcescens (1%). Acquired resistance was carried by E. coli (11% of all E. coli isolates) and E. cloacae (3% of all E. cloacae) isolates, while intrinsic resistance was detected in all the other resistant isolates (n = 31); K. variicola, K. oxytoca, K. pneumoniae, E. cloacae, K. aerogenes, S. marcescens and P. mirabilis). Our data show that most strains isolated in protected areas in Gabon are wild type isolates and carry intrinsic resistance rather than acquired resistance.
format	article
author	Pierre Philippe Mbehang Nguema Richard Onanga Guy Roger Ndong Atome Jean Jules Tewa Arsène Mabika Mabika Jean Ulrich Muandze Nzambe Jean Constant Obague Mbeang Paul Yannick Bitome Essono François Bretagnolle Sylvain Godreuil
author_facet	Pierre Philippe Mbehang Nguema Richard Onanga Guy Roger Ndong Atome Jean Jules Tewa Arsène Mabika Mabika Jean Ulrich Muandze Nzambe Jean Constant Obague Mbeang Paul Yannick Bitome Essono François Bretagnolle Sylvain Godreuil
author_sort	Pierre Philippe Mbehang Nguema
title	High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
title_short	High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
title_full	High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
title_fullStr	High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
title_full_unstemmed	High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.
title_sort	high level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in gabonese national parks.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/009751dbd6504701b48bcd8cba2e6f44
work_keys_str_mv	AT pierrephilippembehangnguema highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT richardonanga highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT guyrogerndongatome highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT jeanjulestewa highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT arsenemabikamabika highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT jeanulrichmuandzenzambe highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT jeanconstantobaguembeang highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT paulyannickbitomeessono highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT francoisbretagnolle highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks AT sylvaingodreuil highlevelofintrinsicphenotypicantimicrobialresistanceinenterobacteriafromterrestrialwildlifeingabonesenationalparks
_version_	1718375221476458496

High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks.

Ejemplares similares