Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>

A Q fever outbreak on a dairy goat and cattle farm was investigated with regard to the One Health concept. Serum samples and vaginal swabs from goats with different reproductive statuses were collected. Cows, cats, and a dog were investigated with the same sample matrix. The farmer’s family was exam...

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Autores principales:	Benjamin U. Bauer, Michael R. Knittler, T. Louise Herms, Dimitrios Frangoulidis, Svea Matthiesen, Dennis Tappe, Martin Runge, Martin Ganter
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Coxiella burnetii</i> cat cattle dog goat MLVA/VNTR Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/00992b35d5a847d78547a6d2ea81f134
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spelling	oai:doaj.org-article:00992b35d5a847d78547a6d2ea81f1342021-11-25T19:11:53ZMultispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>10.3390/vetsci81102522306-7381https://doaj.org/article/00992b35d5a847d78547a6d2ea81f1342021-10-01T00:00:00Zhttps://www.mdpi.com/2306-7381/8/11/252https://doaj.org/toc/2306-7381A Q fever outbreak on a dairy goat and cattle farm was investigated with regard to the One Health concept. Serum samples and vaginal swabs from goats with different reproductive statuses were collected. Cows, cats, and a dog were investigated with the same sample matrix. The farmer’s family was examined by serum samples. Ruminant sera were analyzed with two phase-specific enzyme-linked immunoassays (ELISAs). Dominant immunoglobulin G (IgG) phase II levels reflected current infections in goats. The cows had high IgG phase I and II levels indicating ongoing infections. Feline, canine, and human sera tested positive by indirect fluorescent antibody test (IFAT). Animal vaginal swabs were analyzed by qPCR to detect <i>C. burnetii</i>, and almost all tested positive. A new cattle-associated <i>C. burnetii</i> genotype C16 was identified by the Multiple-Locus Variable-number tandem repeat Analysis (MLVA/VNTR) from ruminant samples. Additionally, a possible influence of 17ß-estradiol on <i>C. burnetii</i> antibody response was evaluated in goat sera. Goats in early/mid-pregnancy had significantly lower levels of phase-specific IgGs and 17ß-estradiol than goats in late pregnancy. We conclude that the cattle herd may have transmitted <i>C. burnetii</i> to the pregnant goat herd, resulting in a Q fever outbreak with one acute human case. The influence of placentation and maternal pregnancy hormones during pregnancy on the immune response is discussed.Benjamin U. BauerMichael R. KnittlerT. Louise HermsDimitrios FrangoulidisSvea MatthiesenDennis TappeMartin RungeMartin GanterMDPI AGarticle<i>Coxiella burnetii</i>catcattledoggoatMLVA/VNTRVeterinary medicineSF600-1100ENVeterinary Sciences, Vol 8, Iss 252, p 252 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Coxiella burnetii</i> cat cattle dog goat MLVA/VNTR Veterinary medicine SF600-1100
spellingShingle	<i>Coxiella burnetii</i> cat cattle dog goat MLVA/VNTR Veterinary medicine SF600-1100 Benjamin U. Bauer Michael R. Knittler T. Louise Herms Dimitrios Frangoulidis Svea Matthiesen Dennis Tappe Martin Runge Martin Ganter Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
description	A Q fever outbreak on a dairy goat and cattle farm was investigated with regard to the One Health concept. Serum samples and vaginal swabs from goats with different reproductive statuses were collected. Cows, cats, and a dog were investigated with the same sample matrix. The farmer’s family was examined by serum samples. Ruminant sera were analyzed with two phase-specific enzyme-linked immunoassays (ELISAs). Dominant immunoglobulin G (IgG) phase II levels reflected current infections in goats. The cows had high IgG phase I and II levels indicating ongoing infections. Feline, canine, and human sera tested positive by indirect fluorescent antibody test (IFAT). Animal vaginal swabs were analyzed by qPCR to detect <i>C. burnetii</i>, and almost all tested positive. A new cattle-associated <i>C. burnetii</i> genotype C16 was identified by the Multiple-Locus Variable-number tandem repeat Analysis (MLVA/VNTR) from ruminant samples. Additionally, a possible influence of 17ß-estradiol on <i>C. burnetii</i> antibody response was evaluated in goat sera. Goats in early/mid-pregnancy had significantly lower levels of phase-specific IgGs and 17ß-estradiol than goats in late pregnancy. We conclude that the cattle herd may have transmitted <i>C. burnetii</i> to the pregnant goat herd, resulting in a Q fever outbreak with one acute human case. The influence of placentation and maternal pregnancy hormones during pregnancy on the immune response is discussed.
format	article
author	Benjamin U. Bauer Michael R. Knittler T. Louise Herms Dimitrios Frangoulidis Svea Matthiesen Dennis Tappe Martin Runge Martin Ganter
author_facet	Benjamin U. Bauer Michael R. Knittler T. Louise Herms Dimitrios Frangoulidis Svea Matthiesen Dennis Tappe Martin Runge Martin Ganter
author_sort	Benjamin U. Bauer
title	Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
title_short	Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
title_full	Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
title_fullStr	Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
title_full_unstemmed	Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>
title_sort	multispecies q fever outbreak in a mixed dairy goat and cattle farm based on a new bovine-associated genotype of <i>coxiella burnetii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/00992b35d5a847d78547a6d2ea81f134
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Multispecies Q Fever Outbreak in a Mixed Dairy Goat and Cattle Farm Based on a New Bovine-Associated Genotype of <i>Coxiella burnetii</i>

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