USP45 and Spindly are part of the same complex implicated in cell migration

Abstract Ubiquitylation is a protein modification implicated in several cellular processes. This process is reversible by the action of deubiquinating enzymes (DUBs). USP45 is a ubiquitin specific protease about which little is known, aside from roles in DNA damage repair and differentiation of the...

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Autores principales:	Claudia Conte, Eric R. Griffis, Ian Hickson, Ana B. Perez-Oliva
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Ubiquitin Chains Type Ubiquitin Protein Ubiquitylation USP14 Activity Dako Mounting Medium Medicine R Science Q
Acceso en línea:	https://doaj.org/article/009b0e446c014860a8da9ab01d93189f
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spelling	oai:doaj.org-article:009b0e446c014860a8da9ab01d93189f2021-12-02T15:08:54ZUSP45 and Spindly are part of the same complex implicated in cell migration10.1038/s41598-018-32685-82045-2322https://doaj.org/article/009b0e446c014860a8da9ab01d93189f2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32685-8https://doaj.org/toc/2045-2322Abstract Ubiquitylation is a protein modification implicated in several cellular processes. This process is reversible by the action of deubiquinating enzymes (DUBs). USP45 is a ubiquitin specific protease about which little is known, aside from roles in DNA damage repair and differentiation of the vertebrate retina. Here, by using mass spectrometry we have identified Spindly as a new target of USP45. Our data show that Spindly and USP45 are part of the same complex and that their interaction specifically depends on the catalytic activity of USP45. In addition, we describe the type of ubiquitin chains associated with the complex that can be cleaved by USP45, with a preferential activity on K48 ubiquitin chain type and potentially K6. Here, we also show that Spindly is mono-ubiquitylated and this can be specifically removed by USP45 in its active form but not by the catalytic inactive form. Lastly, we identified a new role for USP45 in cell migration, similar to that which was recently described for Spindly.Claudia ConteEric R. GriffisIan HicksonAna B. Perez-OlivaNature PortfolioarticleUbiquitin ChainsType UbiquitinProtein UbiquitylationUSP14 ActivityDako Mounting MediumMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ubiquitin Chains Type Ubiquitin Protein Ubiquitylation USP14 Activity Dako Mounting Medium Medicine R Science Q
spellingShingle	Ubiquitin Chains Type Ubiquitin Protein Ubiquitylation USP14 Activity Dako Mounting Medium Medicine R Science Q Claudia Conte Eric R. Griffis Ian Hickson Ana B. Perez-Oliva USP45 and Spindly are part of the same complex implicated in cell migration
description	Abstract Ubiquitylation is a protein modification implicated in several cellular processes. This process is reversible by the action of deubiquinating enzymes (DUBs). USP45 is a ubiquitin specific protease about which little is known, aside from roles in DNA damage repair and differentiation of the vertebrate retina. Here, by using mass spectrometry we have identified Spindly as a new target of USP45. Our data show that Spindly and USP45 are part of the same complex and that their interaction specifically depends on the catalytic activity of USP45. In addition, we describe the type of ubiquitin chains associated with the complex that can be cleaved by USP45, with a preferential activity on K48 ubiquitin chain type and potentially K6. Here, we also show that Spindly is mono-ubiquitylated and this can be specifically removed by USP45 in its active form but not by the catalytic inactive form. Lastly, we identified a new role for USP45 in cell migration, similar to that which was recently described for Spindly.
format	article
author	Claudia Conte Eric R. Griffis Ian Hickson Ana B. Perez-Oliva
author_facet	Claudia Conte Eric R. Griffis Ian Hickson Ana B. Perez-Oliva
author_sort	Claudia Conte
title	USP45 and Spindly are part of the same complex implicated in cell migration
title_short	USP45 and Spindly are part of the same complex implicated in cell migration
title_full	USP45 and Spindly are part of the same complex implicated in cell migration
title_fullStr	USP45 and Spindly are part of the same complex implicated in cell migration
title_full_unstemmed	USP45 and Spindly are part of the same complex implicated in cell migration
title_sort	usp45 and spindly are part of the same complex implicated in cell migration
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/009b0e446c014860a8da9ab01d93189f
work_keys_str_mv	AT claudiaconte usp45andspindlyarepartofthesamecompleximplicatedincellmigration AT ericrgriffis usp45andspindlyarepartofthesamecompleximplicatedincellmigration AT ianhickson usp45andspindlyarepartofthesamecompleximplicatedincellmigration AT anabperezoliva usp45andspindlyarepartofthesamecompleximplicatedincellmigration
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USP45 and Spindly are part of the same complex implicated in cell migration

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