Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis

Abstract Neosartorya fumigata (Aspergillus fumigatus) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daniel G. Panaccione, Stephanie L. Arnold
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/009d78bbe61a499cb5302863bf403bae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:009d78bbe61a499cb5302863bf403bae
record_format	dspace
spelling	oai:doaj.org-article:009d78bbe61a499cb5302863bf403bae2021-12-02T16:07:56ZErgot alkaloids contribute to virulence in an insect model of invasive aspergillosis10.1038/s41598-017-09107-22045-2322https://doaj.org/article/009d78bbe61a499cb5302863bf403bae2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09107-2https://doaj.org/toc/2045-2322Abstract Neosartorya fumigata (Aspergillus fumigatus) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a branch of the ergot alkaloid pathway called fumigaclavines. The biosynthesis of the N. fumigata ergot alkaloids and their relation to those produced by alternate pathway branches in fungi from the plant-inhabiting Clavicipitaceae have been well-characterised, but the potential role of these alkaloids in animal pathogenesis has not been studied extensively. We investigated the contribution of ergot alkaloids to virulence of N. fumigata by measuring mortality in the model insect Galleria mellonella. Larvae were injected with conidia (asexual spores) of two different wild-type strains of N. fumigata and three different ergot alkaloid mutants derived by previous gene knockouts and differing in ergot alkaloid profiles. Elimination of all ergot alkaloids significantly reduced virulence of N. fumigata in G. mellonella (P < 0.0001). Mutants accumulating intermediates but not the pathway end product fumigaclavine C also were less virulent than the wild type (P < 0.0003). The data indicate that ergot alkaloids contribute to virulence of N. fumigata in this insect model and that fumigaclavine C is important for full virulence.Daniel G. PanaccioneStephanie L. ArnoldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel G. Panaccione Stephanie L. Arnold Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
description	Abstract Neosartorya fumigata (Aspergillus fumigatus) is the most common cause of invasive aspergillosis, a frequently fatal lung disease primarily affecting immunocompromised individuals. This opportunistic fungal pathogen produces several classes of specialised metabolites including products of a branch of the ergot alkaloid pathway called fumigaclavines. The biosynthesis of the N. fumigata ergot alkaloids and their relation to those produced by alternate pathway branches in fungi from the plant-inhabiting Clavicipitaceae have been well-characterised, but the potential role of these alkaloids in animal pathogenesis has not been studied extensively. We investigated the contribution of ergot alkaloids to virulence of N. fumigata by measuring mortality in the model insect Galleria mellonella. Larvae were injected with conidia (asexual spores) of two different wild-type strains of N. fumigata and three different ergot alkaloid mutants derived by previous gene knockouts and differing in ergot alkaloid profiles. Elimination of all ergot alkaloids significantly reduced virulence of N. fumigata in G. mellonella (P < 0.0001). Mutants accumulating intermediates but not the pathway end product fumigaclavine C also were less virulent than the wild type (P < 0.0003). The data indicate that ergot alkaloids contribute to virulence of N. fumigata in this insect model and that fumigaclavine C is important for full virulence.
format	article
author	Daniel G. Panaccione Stephanie L. Arnold
author_facet	Daniel G. Panaccione Stephanie L. Arnold
author_sort	Daniel G. Panaccione
title	Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
title_short	Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
title_full	Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
title_fullStr	Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
title_full_unstemmed	Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
title_sort	ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/009d78bbe61a499cb5302863bf403bae
work_keys_str_mv	AT danielgpanaccione ergotalkaloidscontributetovirulenceinaninsectmodelofinvasiveaspergillosis AT stephanielarnold ergotalkaloidscontributetovirulenceinaninsectmodelofinvasiveaspergillosis
_version_	1718384635429257216

Ergot alkaloids contribute to virulence in an insect model of invasive aspergillosis

Ejemplares similares