A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression

Hadiyah N Green,1,2 Stephanie D Crockett,3 Dmitry V Martyshkin,1 Karan P Singh,2,4 William E Grizzle,2,5 Eben L Rosenthal,2,6 Sergey B Mirov11Department of Physics, Center for Optical Sensors and Spectroscopies, 2Comprehensive Cancer Center, 3Department of Pediatrics, Division of Neonatology, 4Depa...

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Autores principales:	Green HN, Crockett SD, Martyshkin DV, Singh KP, Grizzle WE, Rosenthal EL, Mirov SB
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/009f79116d924c95965667e24b3d7e8b
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spelling	oai:doaj.org-article:009f79116d924c95965667e24b3d7e8b2021-12-02T03:11:32ZA histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression1178-2013https://doaj.org/article/009f79116d924c95965667e24b3d7e8b2014-11-01T00:00:00Zhttp://www.dovepress.com/a-histological-evaluation-and-in-vivo-assessment-of-intratumoral-near--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Hadiyah N Green,1,2 Stephanie D Crockett,3 Dmitry V Martyshkin,1 Karan P Singh,2,4 William E Grizzle,2,5 Eben L Rosenthal,2,6 Sergey B Mirov11Department of Physics, Center for Optical Sensors and Spectroscopies, 2Comprehensive Cancer Center, 3Department of Pediatrics, Division of Neonatology, 4Department of Medicine, Division of Preventive Medicine, Biostatistics and Bioinformatics Shared Facility, 5Department of Pathology, 6Department of Surgery, Division of Otolaryngology, Head and Neck Surgery, The University of Alabama at Birmingham, Birmingham, AL, USAPurpose: Nanoparticle (NP)-enabled near infrared (NIR) photothermal therapy has realized limited success in in vivo studies as a potential localized cancer therapy. This is primarily due to a lack of successful methods that can prevent NP uptake by the reticuloendothelial system, especially the liver and kidney, and deliver sufficient quantities of intravenously injected NPs to the tumor site. Histological evaluation of photothermal therapy-induced tumor regression is also neglected in the current literature. This report demonstrates and histologically evaluates the in vivo potential of NIR photothermal therapy by circumventing the challenges of intravenous NP delivery and tumor targeting found in other photothermal therapy studies.Methods: Subcutaneous Cal 27 squamous cell carcinoma xenografts received photothermal nanotherapy treatments, radial injections of polyethylene glycol (PEG)-ylated gold nanorods and one NIR 785 nm laser irradiation for 10 minutes at 9.5 W/cm2. Tumor response was measured for 10–15 days, gross changes in tumor size were evaluated, and the remaining tumors or scar tissues were excised and histologically analyzed.Results: The single treatment of intratumoral nanorod injections followed by a 10 minute NIR laser treatment also known as photothermal nanotherapy, resulted in ~100% tumor regression in ~90% of treated tumors, which was statistically significant in a comparison to the average of all three control groups over time (P<0.01).Conclusion: Photothermal nanotherapy, or intratumoral nanorod injections followed by NIR laser irradiation of tumors and tumor margins, demonstrate the potential of NIR photothermal therapy as a viable localized treatment approach for primary and early stage tumors, and prevents NP uptake by the reticuloendothelial system.Keywords: photothermal cancer therapy, malignancy, cancer treatment, intratumoral, gold nanorods, nanoparticles, PEGylation, laser therapyGreen HNCrockett SDMartyshkin DVSingh KPGrizzle WERosenthal ELMirov SBDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5093-5102 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Green HN Crockett SD Martyshkin DV Singh KP Grizzle WE Rosenthal EL Mirov SB A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
description	Hadiyah N Green,1,2 Stephanie D Crockett,3 Dmitry V Martyshkin,1 Karan P Singh,2,4 William E Grizzle,2,5 Eben L Rosenthal,2,6 Sergey B Mirov11Department of Physics, Center for Optical Sensors and Spectroscopies, 2Comprehensive Cancer Center, 3Department of Pediatrics, Division of Neonatology, 4Department of Medicine, Division of Preventive Medicine, Biostatistics and Bioinformatics Shared Facility, 5Department of Pathology, 6Department of Surgery, Division of Otolaryngology, Head and Neck Surgery, The University of Alabama at Birmingham, Birmingham, AL, USAPurpose: Nanoparticle (NP)-enabled near infrared (NIR) photothermal therapy has realized limited success in in vivo studies as a potential localized cancer therapy. This is primarily due to a lack of successful methods that can prevent NP uptake by the reticuloendothelial system, especially the liver and kidney, and deliver sufficient quantities of intravenously injected NPs to the tumor site. Histological evaluation of photothermal therapy-induced tumor regression is also neglected in the current literature. This report demonstrates and histologically evaluates the in vivo potential of NIR photothermal therapy by circumventing the challenges of intravenous NP delivery and tumor targeting found in other photothermal therapy studies.Methods: Subcutaneous Cal 27 squamous cell carcinoma xenografts received photothermal nanotherapy treatments, radial injections of polyethylene glycol (PEG)-ylated gold nanorods and one NIR 785 nm laser irradiation for 10 minutes at 9.5 W/cm2. Tumor response was measured for 10–15 days, gross changes in tumor size were evaluated, and the remaining tumors or scar tissues were excised and histologically analyzed.Results: The single treatment of intratumoral nanorod injections followed by a 10 minute NIR laser treatment also known as photothermal nanotherapy, resulted in ~100% tumor regression in ~90% of treated tumors, which was statistically significant in a comparison to the average of all three control groups over time (P<0.01).Conclusion: Photothermal nanotherapy, or intratumoral nanorod injections followed by NIR laser irradiation of tumors and tumor margins, demonstrate the potential of NIR photothermal therapy as a viable localized treatment approach for primary and early stage tumors, and prevents NP uptake by the reticuloendothelial system.Keywords: photothermal cancer therapy, malignancy, cancer treatment, intratumoral, gold nanorods, nanoparticles, PEGylation, laser therapy
format	article
author	Green HN Crockett SD Martyshkin DV Singh KP Grizzle WE Rosenthal EL Mirov SB
author_facet	Green HN Crockett SD Martyshkin DV Singh KP Grizzle WE Rosenthal EL Mirov SB
author_sort	Green HN
title	A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
title_short	A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
title_full	A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
title_fullStr	A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
title_full_unstemmed	A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
title_sort	histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/009f79116d924c95965667e24b3d7e8b
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A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression

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