ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.

<h4>Background</h4>Protective responses against Mycobacterium tuberculosis are dependent on appropriate T cell and macrophage activation. Mycobacterial antigen six kDa early secreted antigenic target (ESAT6) and culture filtrate protein 10 (CFP10) can detect M. tuberculosis specific IFNg...

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Autores principales:	Zahra Hasan, Bushra Jamil, Mussarat Ashraf, Muniba Islam, Muhammad S Yusuf, Javaid A Khan, Rabia Hussain
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/009fdc07e45249698703023c0b49bfbc
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spelling	oai:doaj.org-article:009fdc07e45249698703023c0b49bfbc2021-11-25T06:16:22ZESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.1932-620310.1371/journal.pone.0005158https://doaj.org/article/009fdc07e45249698703023c0b49bfbc2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19340290/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Protective responses against Mycobacterium tuberculosis are dependent on appropriate T cell and macrophage activation. Mycobacterial antigen six kDa early secreted antigenic target (ESAT6) and culture filtrate protein 10 (CFP10) can detect M. tuberculosis specific IFNgamma responses. However, most studies have been performed in non-endemic regions and to study pulmonary tuberculosis (PTB). We have studied ESAT6 and CFP10 induced cytokine and chemokines responses in PTB and extrapulmonary (EPul) TB.<h4>Methodology</h4>IFNgamma, IL10, CXCL9 and CCL2 responses were determined using an ex vivo whole blood assay system in PTB (n = 30) and EPulTB patients with limited (LNTB, n = 24) or severe (SevTB, n = 22) disease, and in healthy endemic controls (ECs). Responses to bacterial LPS were also determined.<h4>Principal findings</h4>ESAT6- and CFP10-induced IFNgamma was comparable between ECs and TB patients. Both ESAT6- and CFP10-induced IFNgamma secretion was greater in LNTB than PTB. ESAT6-induced CXCL9 was greater in EPulTB as compared with PTB, with an increase in SevTB as compared with LNTB. CFP10-induced CCL2 was higher in PTB than LNTB patients. LPS-stimulated CXCL9 was greatest in SevTB and LPS-induced CCL2 was increased in PTB as compared with LNTB patients. A positive correlation between ESAT6-induced IFNgamma and CXCL9 was present in all TB patients, but IFNgamma and CCL2 was only correlated in LNTB. ESAT-induced CCL2 and CXCL9 were significantly associated in LNTB while correlation in response to LPS was only present in SevTB.<h4>Conclusions</h4>ESAT6 induced IFNgamma and CXCL9 can differentiate between limited and severe TB infections.Zahra HasanBushra JamilMussarat AshrafMuniba IslamMuhammad S YusufJavaid A KhanRabia HussainPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5158 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zahra Hasan Bushra Jamil Mussarat Ashraf Muniba Islam Muhammad S Yusuf Javaid A Khan Rabia Hussain ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
description	<h4>Background</h4>Protective responses against Mycobacterium tuberculosis are dependent on appropriate T cell and macrophage activation. Mycobacterial antigen six kDa early secreted antigenic target (ESAT6) and culture filtrate protein 10 (CFP10) can detect M. tuberculosis specific IFNgamma responses. However, most studies have been performed in non-endemic regions and to study pulmonary tuberculosis (PTB). We have studied ESAT6 and CFP10 induced cytokine and chemokines responses in PTB and extrapulmonary (EPul) TB.<h4>Methodology</h4>IFNgamma, IL10, CXCL9 and CCL2 responses were determined using an ex vivo whole blood assay system in PTB (n = 30) and EPulTB patients with limited (LNTB, n = 24) or severe (SevTB, n = 22) disease, and in healthy endemic controls (ECs). Responses to bacterial LPS were also determined.<h4>Principal findings</h4>ESAT6- and CFP10-induced IFNgamma was comparable between ECs and TB patients. Both ESAT6- and CFP10-induced IFNgamma secretion was greater in LNTB than PTB. ESAT6-induced CXCL9 was greater in EPulTB as compared with PTB, with an increase in SevTB as compared with LNTB. CFP10-induced CCL2 was higher in PTB than LNTB patients. LPS-stimulated CXCL9 was greatest in SevTB and LPS-induced CCL2 was increased in PTB as compared with LNTB patients. A positive correlation between ESAT6-induced IFNgamma and CXCL9 was present in all TB patients, but IFNgamma and CCL2 was only correlated in LNTB. ESAT-induced CCL2 and CXCL9 were significantly associated in LNTB while correlation in response to LPS was only present in SevTB.<h4>Conclusions</h4>ESAT6 induced IFNgamma and CXCL9 can differentiate between limited and severe TB infections.
format	article
author	Zahra Hasan Bushra Jamil Mussarat Ashraf Muniba Islam Muhammad S Yusuf Javaid A Khan Rabia Hussain
author_facet	Zahra Hasan Bushra Jamil Mussarat Ashraf Muniba Islam Muhammad S Yusuf Javaid A Khan Rabia Hussain
author_sort	Zahra Hasan
title	ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
title_short	ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
title_full	ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
title_fullStr	ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
title_full_unstemmed	ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.
title_sort	esat6-induced ifngamma and cxcl9 can differentiate severity of tuberculosis.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/009fdc07e45249698703023c0b49bfbc
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ESAT6-induced IFNgamma and CXCL9 can differentiate severity of tuberculosis.

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