Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.

Refined vaccines and adjuvants are urgently needed to advance immunization against global infectious challenges such as HIV, hepatitis C, tuberculosis and malaria. Large-scale screening efforts are ongoing to identify adjuvants with improved efficacy profiles. Reactogenicity often represents a major...

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Auteurs principaux:	Margarete Arras, Daniel L Glauser, Paulin Jirkof, Andreas Rettich, Benjamin Schade, Paolo Cinelli, Daniel D Pinschewer, Mathias Ackermann
Format:	article
Langue:	EN
Publié:	Public Library of Science (PLoS) 2012
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/00a5b32e0e794d43ad96b8e5939a4141
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spelling	oai:doaj.org-article:00a5b32e0e794d43ad96b8e5939a41412021-11-18T07:29:51ZMultiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.1932-620310.1371/journal.pone.0029726https://doaj.org/article/00a5b32e0e794d43ad96b8e5939a41412012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22276127/?tool=EBIhttps://doaj.org/toc/1932-6203Refined vaccines and adjuvants are urgently needed to advance immunization against global infectious challenges such as HIV, hepatitis C, tuberculosis and malaria. Large-scale screening efforts are ongoing to identify adjuvants with improved efficacy profiles. Reactogenicity often represents a major hurdle to the clinical use of new substances. Yet, irrespective of its importance, this parameter has remained difficult to screen for, owing to a lack of sensitive small animal models with a capacity for high throughput testing. Here we report that continuous telemetric measurements of heart rate, heart rate variability, body core temperature and locomotor activity in laboratory mice readily unmasked systemic side-effects of vaccination, which went undetected by conventional observational assessment and clinical scoring. Even minor aberrations in homeostasis were readily detected, ranging from sympathetic activation over transient pyrogenic effects to reduced physical activity and apathy. Results in real-time combined with the potential of scalability and partial automation in the industrial context suggest multiparameter telemetry in laboratory mice as a first-line screen for vaccine reactogenicity. This may accelerate vaccine discovery in general and may further the success of vaccines in combating infectious disease and cancer.Margarete ArrasDaniel L GlauserPaulin JirkofAndreas RettichBenjamin SchadePaolo CinelliDaniel D PinschewerMathias AckermannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29726 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Margarete Arras Daniel L Glauser Paulin Jirkof Andreas Rettich Benjamin Schade Paolo Cinelli Daniel D Pinschewer Mathias Ackermann Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
description	Refined vaccines and adjuvants are urgently needed to advance immunization against global infectious challenges such as HIV, hepatitis C, tuberculosis and malaria. Large-scale screening efforts are ongoing to identify adjuvants with improved efficacy profiles. Reactogenicity often represents a major hurdle to the clinical use of new substances. Yet, irrespective of its importance, this parameter has remained difficult to screen for, owing to a lack of sensitive small animal models with a capacity for high throughput testing. Here we report that continuous telemetric measurements of heart rate, heart rate variability, body core temperature and locomotor activity in laboratory mice readily unmasked systemic side-effects of vaccination, which went undetected by conventional observational assessment and clinical scoring. Even minor aberrations in homeostasis were readily detected, ranging from sympathetic activation over transient pyrogenic effects to reduced physical activity and apathy. Results in real-time combined with the potential of scalability and partial automation in the industrial context suggest multiparameter telemetry in laboratory mice as a first-line screen for vaccine reactogenicity. This may accelerate vaccine discovery in general and may further the success of vaccines in combating infectious disease and cancer.
format	article
author	Margarete Arras Daniel L Glauser Paulin Jirkof Andreas Rettich Benjamin Schade Paolo Cinelli Daniel D Pinschewer Mathias Ackermann
author_facet	Margarete Arras Daniel L Glauser Paulin Jirkof Andreas Rettich Benjamin Schade Paolo Cinelli Daniel D Pinschewer Mathias Ackermann
author_sort	Margarete Arras
title	Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
title_short	Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
title_full	Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
title_fullStr	Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
title_full_unstemmed	Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
title_sort	multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/00a5b32e0e794d43ad96b8e5939a4141
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Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice.

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