Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a nov...
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| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/00a855e90bea4385a689fc360575c967 |
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| Sumario: | Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a novel rapeseed accession with up-curling leaf, analyzed the up-curling leaf trait inheritance, and fine mapped the locus for up-curling leaf property (<i>Bnuc3)</i> in <i>Brassica napus</i>. Genetic analysis revealed that the up-curling leaf trait is controlled by a single dominant locus, named <i>BnUC3</i>. We performed an association study of <i>BnUC3</i> with single nucleotide polymorphism (SNP) markers using a backcross population derived from the homozygous up-curling leaf line NJAU-M1295 and the canola variety ‘zhongshuang11’ with typical flat leaves, and mapped the <i>BnUC3</i> locus in a 1.92 Mb interval of chromosome A02 of <i>B. napus</i>. To further map <i>BnUC3</i>, 232 simple sequence repeat (SSR) primers and four pairs of Insertion/Deletion (InDel) primers were developed for the mapping interval. Among them, five SSR markers and two InDel markers were polymorphic. By these markers, the mapping interval was narrowed to 92.0 kb using another F<sub>2</sub> population. This fine mapping interval has 11 annotated genes among which <i>BnaA02T0157000ZS</i> were inferred to be candidate casual genes for up-curling leaf based on the cloned sequence analysis, gene functionality, and gene expression analysis. The current study laid a foundational basis for further elucidating the mechanism of <i>BnUC3</i> and breeding of variety with up-curling leaf. |
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