Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>

Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a nov...

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Autores principales:	Shubei Wan, Zongping Qin, Xiaomei Jiang, Mao Yang, Wenjing Chen, Yangming Wang, Fei Ni, Yijian Guan, Rongzhan Guan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	up-curling leaf mapping <i>Brassica napus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/00a855e90bea4385a689fc360575c967
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spelling	oai:doaj.org-article:00a855e90bea4385a689fc360575c9672021-11-11T17:09:30ZIdentification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>10.3390/ijms2221116931422-00671661-6596https://doaj.org/article/00a855e90bea4385a689fc360575c9672021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11693https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a novel rapeseed accession with up-curling leaf, analyzed the up-curling leaf trait inheritance, and fine mapped the locus for up-curling leaf property (<i>Bnuc3)</i> in <i>Brassica napus</i>. Genetic analysis revealed that the up-curling leaf trait is controlled by a single dominant locus, named <i>BnUC3</i>. We performed an association study of <i>BnUC3</i> with single nucleotide polymorphism (SNP) markers using a backcross population derived from the homozygous up-curling leaf line NJAU-M1295 and the canola variety ‘zhongshuang11’ with typical flat leaves, and mapped the <i>BnUC3</i> locus in a 1.92 Mb interval of chromosome A02 of <i>B. napus</i>. To further map <i>BnUC3</i>, 232 simple sequence repeat (SSR) primers and four pairs of Insertion/Deletion (InDel) primers were developed for the mapping interval. Among them, five SSR markers and two InDel markers were polymorphic. By these markers, the mapping interval was narrowed to 92.0 kb using another F<sub>2</sub> population. This fine mapping interval has 11 annotated genes among which <i>BnaA02T0157000ZS</i> were inferred to be candidate casual genes for up-curling leaf based on the cloned sequence analysis, gene functionality, and gene expression analysis. The current study laid a foundational basis for further elucidating the mechanism of <i>BnUC3</i> and breeding of variety with up-curling leaf.Shubei WanZongping QinXiaomei JiangMao YangWenjing ChenYangming WangFei NiYijian GuanRongzhan GuanMDPI AGarticleup-curling leafmapping<i>Brassica napus</i> L.Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11693, p 11693 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	up-curling leaf mapping <i>Brassica napus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	up-curling leaf mapping <i>Brassica napus</i> L. Biology (General) QH301-705.5 Chemistry QD1-999 Shubei Wan Zongping Qin Xiaomei Jiang Mao Yang Wenjing Chen Yangming Wang Fei Ni Yijian Guan Rongzhan Guan Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
description	Leaf trait is an important target trait in crop breeding programs. Moderate leaf curling may be a help for improving crop yield by minimizing the shadowing by leaves. Mining locus for leaf curling trait is of significance for plant genetics and breeding researches. The present study identified a novel rapeseed accession with up-curling leaf, analyzed the up-curling leaf trait inheritance, and fine mapped the locus for up-curling leaf property (<i>Bnuc3)</i> in <i>Brassica napus</i>. Genetic analysis revealed that the up-curling leaf trait is controlled by a single dominant locus, named <i>BnUC3</i>. We performed an association study of <i>BnUC3</i> with single nucleotide polymorphism (SNP) markers using a backcross population derived from the homozygous up-curling leaf line NJAU-M1295 and the canola variety ‘zhongshuang11’ with typical flat leaves, and mapped the <i>BnUC3</i> locus in a 1.92 Mb interval of chromosome A02 of <i>B. napus</i>. To further map <i>BnUC3</i>, 232 simple sequence repeat (SSR) primers and four pairs of Insertion/Deletion (InDel) primers were developed for the mapping interval. Among them, five SSR markers and two InDel markers were polymorphic. By these markers, the mapping interval was narrowed to 92.0 kb using another F<sub>2</sub> population. This fine mapping interval has 11 annotated genes among which <i>BnaA02T0157000ZS</i> were inferred to be candidate casual genes for up-curling leaf based on the cloned sequence analysis, gene functionality, and gene expression analysis. The current study laid a foundational basis for further elucidating the mechanism of <i>BnUC3</i> and breeding of variety with up-curling leaf.
format	article
author	Shubei Wan Zongping Qin Xiaomei Jiang Mao Yang Wenjing Chen Yangming Wang Fei Ni Yijian Guan Rongzhan Guan
author_facet	Shubei Wan Zongping Qin Xiaomei Jiang Mao Yang Wenjing Chen Yangming Wang Fei Ni Yijian Guan Rongzhan Guan
author_sort	Shubei Wan
title	Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
title_short	Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
title_full	Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
title_fullStr	Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
title_full_unstemmed	Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>
title_sort	identification and fine mapping of a locus related to leaf up-curling trait (bnuc3) in <i>brassica napus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/00a855e90bea4385a689fc360575c967
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Identification and Fine Mapping of a Locus Related to Leaf Up-Curling Trait (Bnuc3) in <i>Brassica napus</i>

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