Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase

Abstract Many types of adherent cells are known to reorient upon uniaxial cyclic stretching perpendicularly to the direction of stretching to facilitate such important events as wound healing, angiogenesis, and morphogenesis. While this phenomenon has been documented for decades, the underlying mech...

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Autores principales:	Jui-Chien Lien, Yu-li Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00b5863c87f54f2da75f87b3ccb092d1
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spelling	oai:doaj.org-article:00b5863c87f54f2da75f87b3ccb092d12021-12-02T17:03:49ZCyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase10.1038/s41598-021-93987-y2045-2322https://doaj.org/article/00b5863c87f54f2da75f87b3ccb092d12021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93987-yhttps://doaj.org/toc/2045-2322Abstract Many types of adherent cells are known to reorient upon uniaxial cyclic stretching perpendicularly to the direction of stretching to facilitate such important events as wound healing, angiogenesis, and morphogenesis. While this phenomenon has been documented for decades, the underlying mechanism remains poorly understood. Using an on-stage stretching device that allowed programmable stretching with synchronized imaging, we found that the reorientation of NRK epithelial cells took place primarily during the relaxation phase when cells underwent rapid global retraction followed by extension transverse to the direction of stretching. Inhibition of myosin II caused cells to orient along the direction of stretching, whereas disassembly of microtubules enhanced transverse reorientation. Our results indicate distinct roles of stretching and relaxation in cell reorientation and implicate a role of myosin II-dependent contraction via a microtubule-modulated mechanism. The importance of relaxation phase also explains the difference between the responses to cyclic and static stretching.Jui-Chien LienYu-li WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jui-Chien Lien Yu-li Wang Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
description	Abstract Many types of adherent cells are known to reorient upon uniaxial cyclic stretching perpendicularly to the direction of stretching to facilitate such important events as wound healing, angiogenesis, and morphogenesis. While this phenomenon has been documented for decades, the underlying mechanism remains poorly understood. Using an on-stage stretching device that allowed programmable stretching with synchronized imaging, we found that the reorientation of NRK epithelial cells took place primarily during the relaxation phase when cells underwent rapid global retraction followed by extension transverse to the direction of stretching. Inhibition of myosin II caused cells to orient along the direction of stretching, whereas disassembly of microtubules enhanced transverse reorientation. Our results indicate distinct roles of stretching and relaxation in cell reorientation and implicate a role of myosin II-dependent contraction via a microtubule-modulated mechanism. The importance of relaxation phase also explains the difference between the responses to cyclic and static stretching.
format	article
author	Jui-Chien Lien Yu-li Wang
author_facet	Jui-Chien Lien Yu-li Wang
author_sort	Jui-Chien Lien
title	Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
title_short	Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
title_full	Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
title_fullStr	Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
title_full_unstemmed	Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
title_sort	cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/00b5863c87f54f2da75f87b3ccb092d1
work_keys_str_mv	AT juichienlien cyclicstretchinginducedepithelialcellreorientationisdrivenbymicrotubulemodulatedtransverseextensionduringtherelaxationphase AT yuliwang cyclicstretchinginducedepithelialcellreorientationisdrivenbymicrotubulemodulatedtransverseextensionduringtherelaxationphase
_version_	1718381887787892736

Cyclic stretching-induced epithelial cell reorientation is driven by microtubule-modulated transverse extension during the relaxation phase

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