Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy

Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy

Abstract Now more than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome. Cellular energetics can be impacted by many bacteriostatic and bactericidal antibiotics, which affect metabolism and ene...

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Autores principales: Helen Smith, Sharon Grant, Joanne Parker, Richard Murphy
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/00bfef374d9e4160b1b38a9ba4419377
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spelling oai:doaj.org-article:00bfef374d9e4160b1b38a9ba44193772021-12-02T12:40:41ZYeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy10.1038/s41598-020-78855-52045-2322https://doaj.org/article/00bfef374d9e4160b1b38a9ba44193772020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78855-5https://doaj.org/toc/2045-2322Abstract Now more than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome. Cellular energetics can be impacted by many bacteriostatic and bactericidal antibiotics, which affect metabolism and energy output, resulting in a reduction of cell growth or induction of cell death respectively. In this study, we provide evidence that a mannan rich fraction (MRF) from the cell wall of Saccharomyces cerevisiae modulates growth of antibiotic susceptible and resistant Escherichia coli and potentiates bactericidal antibiotic efficiency through modulation of bacterial cellular respiration. The role of MRF in modulating bactericidal impact and cellular metabolic state were assessed in E. coli by monitoring microbial growth and by measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XFe96 Analyser, respectively. This work further illustrates the link between bacterial susceptibility to antibiotics (phenotypic resistance) and resistance through modulation of bacterial metabolism. This is the first example of yeast MRF enabling collateral sensitivity to antibiotics in vitro and supports the search for alternative strategies to promote animal health without contributing to the growing issue of antimicrobial resistance.Helen SmithSharon GrantJoanne ParkerRichard MurphyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Helen Smith
Sharon Grant
Joanne Parker
Richard Murphy
Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
description Abstract Now more than ever there is a demand to understand the mechanisms surrounding antibiotic resistance and look for alternative ways to impact phenotypic antibiotic outcome. Cellular energetics can be impacted by many bacteriostatic and bactericidal antibiotics, which affect metabolism and energy output, resulting in a reduction of cell growth or induction of cell death respectively. In this study, we provide evidence that a mannan rich fraction (MRF) from the cell wall of Saccharomyces cerevisiae modulates growth of antibiotic susceptible and resistant Escherichia coli and potentiates bactericidal antibiotic efficiency through modulation of bacterial cellular respiration. The role of MRF in modulating bactericidal impact and cellular metabolic state were assessed in E. coli by monitoring microbial growth and by measuring oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using the Seahorse XFe96 Analyser, respectively. This work further illustrates the link between bacterial susceptibility to antibiotics (phenotypic resistance) and resistance through modulation of bacterial metabolism. This is the first example of yeast MRF enabling collateral sensitivity to antibiotics in vitro and supports the search for alternative strategies to promote animal health without contributing to the growing issue of antimicrobial resistance.
format article
author Helen Smith
Sharon Grant
Joanne Parker
Richard Murphy
author_facet Helen Smith
Sharon Grant
Joanne Parker
Richard Murphy
author_sort Helen Smith
title Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
title_short Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
title_full Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
title_fullStr Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
title_full_unstemmed Yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
title_sort yeast cell wall mannan rich fraction modulates bacterial cellular respiration potentiating antibiotic efficacy
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/00bfef374d9e4160b1b38a9ba4419377
work_keys_str_mv AT helensmith yeastcellwallmannanrichfractionmodulatesbacterialcellularrespirationpotentiatingantibioticefficacy
AT sharongrant yeastcellwallmannanrichfractionmodulatesbacterialcellularrespirationpotentiatingantibioticefficacy
AT joanneparker yeastcellwallmannanrichfractionmodulatesbacterialcellularrespirationpotentiatingantibioticefficacy
AT richardmurphy yeastcellwallmannanrichfractionmodulatesbacterialcellularrespirationpotentiatingantibioticefficacy
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