Epitachophoresis is a novel versatile total nucleic acid extraction method

Abstract Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extract...

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Autores principales: Vladimira Datinska, Pantea Gheibi, Keynttisha Jefferson, Jaeyoung Yang, Sri Paladugu, Carolina Dallett, Ivona Voracova, Frantisek Foret, Yann Astier
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/00c39e87922a484680bbb26c4758cffb
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spelling oai:doaj.org-article:00c39e87922a484680bbb26c4758cffb2021-11-28T12:20:22ZEpitachophoresis is a novel versatile total nucleic acid extraction method10.1038/s41598-021-02214-12045-2322https://doaj.org/article/00c39e87922a484680bbb26c4758cffb2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02214-1https://doaj.org/toc/2045-2322Abstract Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.Vladimira DatinskaPantea GheibiKeynttisha JeffersonJaeyoung YangSri PaladuguCarolina DallettIvona VoracovaFrantisek ForetYann AstierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Vladimira Datinska
Pantea Gheibi
Keynttisha Jefferson
Jaeyoung Yang
Sri Paladugu
Carolina Dallett
Ivona Voracova
Frantisek Foret
Yann Astier
Epitachophoresis is a novel versatile total nucleic acid extraction method
description Abstract Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
format article
author Vladimira Datinska
Pantea Gheibi
Keynttisha Jefferson
Jaeyoung Yang
Sri Paladugu
Carolina Dallett
Ivona Voracova
Frantisek Foret
Yann Astier
author_facet Vladimira Datinska
Pantea Gheibi
Keynttisha Jefferson
Jaeyoung Yang
Sri Paladugu
Carolina Dallett
Ivona Voracova
Frantisek Foret
Yann Astier
author_sort Vladimira Datinska
title Epitachophoresis is a novel versatile total nucleic acid extraction method
title_short Epitachophoresis is a novel versatile total nucleic acid extraction method
title_full Epitachophoresis is a novel versatile total nucleic acid extraction method
title_fullStr Epitachophoresis is a novel versatile total nucleic acid extraction method
title_full_unstemmed Epitachophoresis is a novel versatile total nucleic acid extraction method
title_sort epitachophoresis is a novel versatile total nucleic acid extraction method
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/00c39e87922a484680bbb26c4758cffb
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