A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.

Many pesticides are used increasingly in combinations during crop protection and their stability ensures the presence of such combinations in foodstuffs. The effects of three fungicides, pyrimethanil, cyprodinil and fludioxonil, were investigated together and separately on U251 and SH-SY5Y cells, wh...

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Autores principales:	Michael D Coleman, John D O'Neil, Elizabeth K Woehrling, Oscar Bate Akide Ndunge, Eric J Hill, Andre Menache, Claude J Reiss
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00cc83d1ac624ae9b508d2485a088016
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spelling	oai:doaj.org-article:00cc83d1ac624ae9b508d2485a0880162021-11-18T07:09:39ZA preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.1932-620310.1371/journal.pone.0042768https://doaj.org/article/00cc83d1ac624ae9b508d2485a0880162012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880100/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many pesticides are used increasingly in combinations during crop protection and their stability ensures the presence of such combinations in foodstuffs. The effects of three fungicides, pyrimethanil, cyprodinil and fludioxonil, were investigated together and separately on U251 and SH-SY5Y cells, which can be representative of human CNS glial and neuronal cells respectively. Over 48h, all three agents showed significant reductions in cellular ATP, at concentrations that were more than tenfold lower than those which significantly impaired cellular viability. The effects on energy metabolism were reflected in their marked toxic effects on mitochondrial membrane potential. In addition, evidence of oxidative stress was seen in terms of a fall in cellular thiols coupled with increases in the expression of enzymes associated with reactive species formation, such as GSH peroxidase and superoxide dismutase. The glial cell line showed significant responsiveness to the toxin challenge in terms of changes in antioxidant gene expression, although the neuronal SH-SY5Y line exhibited greater vulnerability to toxicity, which was reflected in significant increases in caspase-3 expression, which is indicative of the initiation of apoptosis. Cyprodinil was the most toxic agent individually, although oxidative stress-related enzyme gene expression increases appeared to demonstrate some degree of synergy in the presence of the combination of agents. This report suggests that the impact of some pesticides, both individually and in combinations, merits further study in terms of their impact on human cellular health.Michael D ColemanJohn D O'NeilElizabeth K WoehrlingOscar Bate Akide NdungeEric J HillAndre MenacheClaude J ReissPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42768 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael D Coleman John D O'Neil Elizabeth K Woehrling Oscar Bate Akide Ndunge Eric J Hill Andre Menache Claude J Reiss A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
description	Many pesticides are used increasingly in combinations during crop protection and their stability ensures the presence of such combinations in foodstuffs. The effects of three fungicides, pyrimethanil, cyprodinil and fludioxonil, were investigated together and separately on U251 and SH-SY5Y cells, which can be representative of human CNS glial and neuronal cells respectively. Over 48h, all three agents showed significant reductions in cellular ATP, at concentrations that were more than tenfold lower than those which significantly impaired cellular viability. The effects on energy metabolism were reflected in their marked toxic effects on mitochondrial membrane potential. In addition, evidence of oxidative stress was seen in terms of a fall in cellular thiols coupled with increases in the expression of enzymes associated with reactive species formation, such as GSH peroxidase and superoxide dismutase. The glial cell line showed significant responsiveness to the toxin challenge in terms of changes in antioxidant gene expression, although the neuronal SH-SY5Y line exhibited greater vulnerability to toxicity, which was reflected in significant increases in caspase-3 expression, which is indicative of the initiation of apoptosis. Cyprodinil was the most toxic agent individually, although oxidative stress-related enzyme gene expression increases appeared to demonstrate some degree of synergy in the presence of the combination of agents. This report suggests that the impact of some pesticides, both individually and in combinations, merits further study in terms of their impact on human cellular health.
format	article
author	Michael D Coleman John D O'Neil Elizabeth K Woehrling Oscar Bate Akide Ndunge Eric J Hill Andre Menache Claude J Reiss
author_facet	Michael D Coleman John D O'Neil Elizabeth K Woehrling Oscar Bate Akide Ndunge Eric J Hill Andre Menache Claude J Reiss
author_sort	Michael D Coleman
title	A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
title_short	A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
title_full	A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
title_fullStr	A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
title_full_unstemmed	A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
title_sort	preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/00cc83d1ac624ae9b508d2485a088016
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A preliminary investigation into the impact of a pesticide combination on human neuronal and glial cell lines in vitro.

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