A novel mode of cytokinesis without cell-substratum adhesion

Abstract Cytokinesis is a final step in cell division. Dictyostelium cells, a model organism for the study of cytokinesis, have multiple modes, denoted cytokinesis A, B, C, and D. All these modes have been mainly investigated using cells adhering to the substratum although they can grow in shaking s...

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Autores principales:	Risa Taira, Shigehiko Yumura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00d2ccac69db49738039caab7b4fea07
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spelling	oai:doaj.org-article:00d2ccac69db49738039caab7b4fea072021-12-02T15:05:09ZA novel mode of cytokinesis without cell-substratum adhesion10.1038/s41598-017-17477-w2045-2322https://doaj.org/article/00d2ccac69db49738039caab7b4fea072017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17477-whttps://doaj.org/toc/2045-2322Abstract Cytokinesis is a final step in cell division. Dictyostelium cells, a model organism for the study of cytokinesis, have multiple modes, denoted cytokinesis A, B, C, and D. All these modes have been mainly investigated using cells adhering to the substratum although they can grow in shaking suspension culture. Here, we observed how cells divide without adhering to the substratum using a new non-adhesive material. These detached cells formed the cleavage furrow but eventually failed in the final abscission. Thus, the cells cannot divide without adhesion, suggesting that they cannot divide only through the conventional cytokinesis A. However, in a long-term culture, the detached cells adhered each other to form multicellular aggregates and divided properly in these aggregates. Myosin II-null cells also formed such aggregates but could not divide in the aggregates. Several lines of experiments using mutant cells showed that the process of cytokinesis in multicellular aggregates is a novel mode utilizing a confined space in the aggregate in a myosin II-dependent manner. These results shed light on a poorly characterized mechanism of cytokinesis in multicellular spheroids or tissues. We propose to redefine and classify multiple modes of cytokinesis.Risa TairaShigehiko YumuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Risa Taira Shigehiko Yumura A novel mode of cytokinesis without cell-substratum adhesion
description	Abstract Cytokinesis is a final step in cell division. Dictyostelium cells, a model organism for the study of cytokinesis, have multiple modes, denoted cytokinesis A, B, C, and D. All these modes have been mainly investigated using cells adhering to the substratum although they can grow in shaking suspension culture. Here, we observed how cells divide without adhering to the substratum using a new non-adhesive material. These detached cells formed the cleavage furrow but eventually failed in the final abscission. Thus, the cells cannot divide without adhesion, suggesting that they cannot divide only through the conventional cytokinesis A. However, in a long-term culture, the detached cells adhered each other to form multicellular aggregates and divided properly in these aggregates. Myosin II-null cells also formed such aggregates but could not divide in the aggregates. Several lines of experiments using mutant cells showed that the process of cytokinesis in multicellular aggregates is a novel mode utilizing a confined space in the aggregate in a myosin II-dependent manner. These results shed light on a poorly characterized mechanism of cytokinesis in multicellular spheroids or tissues. We propose to redefine and classify multiple modes of cytokinesis.
format	article
author	Risa Taira Shigehiko Yumura
author_facet	Risa Taira Shigehiko Yumura
author_sort	Risa Taira
title	A novel mode of cytokinesis without cell-substratum adhesion
title_short	A novel mode of cytokinesis without cell-substratum adhesion
title_full	A novel mode of cytokinesis without cell-substratum adhesion
title_fullStr	A novel mode of cytokinesis without cell-substratum adhesion
title_full_unstemmed	A novel mode of cytokinesis without cell-substratum adhesion
title_sort	novel mode of cytokinesis without cell-substratum adhesion
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/00d2ccac69db49738039caab7b4fea07
work_keys_str_mv	AT risataira anovelmodeofcytokinesiswithoutcellsubstratumadhesion AT shigehikoyumura anovelmodeofcytokinesiswithoutcellsubstratumadhesion AT risataira novelmodeofcytokinesiswithoutcellsubstratumadhesion AT shigehikoyumura novelmodeofcytokinesiswithoutcellsubstratumadhesion
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A novel mode of cytokinesis without cell-substratum adhesion

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