Cell-type specific four-component hydrogel.

In the field of regenerative medicine we aim to develop implant matrices for specific tissue needs. By combining two per se, cell-permissive gel systems with enzymatic crosslinkers (gelatin/transglutaminase and fibrinogen/thrombin) to generate a blend (technical term: quattroGel), an unexpected cell...

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Autores principales:	Timo Aberle, Katrin Franke, Elke Rist, Karin Benz, Burkhard Schlosshauer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00ddbcb3a16d478685292aba7cf09baa
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id	oai:doaj.org-article:00ddbcb3a16d478685292aba7cf09baa
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spelling	oai:doaj.org-article:00ddbcb3a16d478685292aba7cf09baa2021-11-18T08:35:39ZCell-type specific four-component hydrogel.1932-620310.1371/journal.pone.0086740https://doaj.org/article/00ddbcb3a16d478685292aba7cf09baa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24475174/?tool=EBIhttps://doaj.org/toc/1932-6203In the field of regenerative medicine we aim to develop implant matrices for specific tissue needs. By combining two per se, cell-permissive gel systems with enzymatic crosslinkers (gelatin/transglutaminase and fibrinogen/thrombin) to generate a blend (technical term: quattroGel), an unexpected cell-selectivity evolved. QuattroGels were porous and formed cavities in the cell diameter range, possessed gelation kinetics in the minute range, viscoelastic properties and a mechanical strength appropriate for general cell adhesion, and restricted diffusion. Cell proliferation of endothelial cells, chondrocytes and fibroblasts was essentially unaffected. In contrast, on quattroGels neither endothelial cells formed vascular tubes nor did primary neurons extend neurites in significant amounts. Only chondrocytes differentiated properly as judged by collagen isoform expression. The biophysical quattroGel characteristics appeared to leave distinct cell processes such as mitosis unaffected and favored differentiation of sessile cells, but hampered differentiation of migratory cells. This cell-type selectivity is of interest e.g. during articular cartilage or invertebral disc repair, where pathological innervation and angiogenesis represent adverse events in tissue engineering.Timo AberleKatrin FrankeElke RistKarin BenzBurkhard SchlosshauerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86740 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Timo Aberle Katrin Franke Elke Rist Karin Benz Burkhard Schlosshauer Cell-type specific four-component hydrogel.
description	In the field of regenerative medicine we aim to develop implant matrices for specific tissue needs. By combining two per se, cell-permissive gel systems with enzymatic crosslinkers (gelatin/transglutaminase and fibrinogen/thrombin) to generate a blend (technical term: quattroGel), an unexpected cell-selectivity evolved. QuattroGels were porous and formed cavities in the cell diameter range, possessed gelation kinetics in the minute range, viscoelastic properties and a mechanical strength appropriate for general cell adhesion, and restricted diffusion. Cell proliferation of endothelial cells, chondrocytes and fibroblasts was essentially unaffected. In contrast, on quattroGels neither endothelial cells formed vascular tubes nor did primary neurons extend neurites in significant amounts. Only chondrocytes differentiated properly as judged by collagen isoform expression. The biophysical quattroGel characteristics appeared to leave distinct cell processes such as mitosis unaffected and favored differentiation of sessile cells, but hampered differentiation of migratory cells. This cell-type selectivity is of interest e.g. during articular cartilage or invertebral disc repair, where pathological innervation and angiogenesis represent adverse events in tissue engineering.
format	article
author	Timo Aberle Katrin Franke Elke Rist Karin Benz Burkhard Schlosshauer
author_facet	Timo Aberle Katrin Franke Elke Rist Karin Benz Burkhard Schlosshauer
author_sort	Timo Aberle
title	Cell-type specific four-component hydrogel.
title_short	Cell-type specific four-component hydrogel.
title_full	Cell-type specific four-component hydrogel.
title_fullStr	Cell-type specific four-component hydrogel.
title_full_unstemmed	Cell-type specific four-component hydrogel.
title_sort	cell-type specific four-component hydrogel.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/00ddbcb3a16d478685292aba7cf09baa
work_keys_str_mv	AT timoaberle celltypespecificfourcomponenthydrogel AT katrinfranke celltypespecificfourcomponenthydrogel AT elkerist celltypespecificfourcomponenthydrogel AT karinbenz celltypespecificfourcomponenthydrogel AT burkhardschlosshauer celltypespecificfourcomponenthydrogel
_version_	1718421541439406080

Cell-type specific four-component hydrogel.

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