Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology

The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to eva...

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Autores principales:	José Alfredo Santiz-Gómez, Miguel Abud-Archila, Víctor Manuel Ruíz-Valdiviezo, Yazmin Sánchez-Roque, Federico Antonio Gutiérrez-Miceli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	carrot encapsulation somatic cells spray drying Botany QK1-989
Acceso en línea:	https://doaj.org/article/00dfbc47924e4efd8e8bdccc1c377b8a
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spelling	oai:doaj.org-article:00dfbc47924e4efd8e8bdccc1c377b8a2021-11-25T18:47:01ZTotipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology10.3390/plants101124912223-7747https://doaj.org/article/00dfbc47924e4efd8e8bdccc1c377b8a2021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2491https://doaj.org/toc/2223-7747The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to evaluate the effect of spray drying on the viability and totipotency of somatic carrot cells. Leaf, root and stem explants were evaluated to induce callus with 2 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Calli obtained from the stem were cultivated in a liquid medium with 1 mg/L of 2,4-D. Cell suspensions were spray dried with maltodextrin-gum Arabic and maltodextrin-xanthan gum mixtures, two outlet air temperatures (50 and 60 °C) and 120 °C inlet air temperature. Results showed that carrot cells were viable after spray drying, and this viability remained for six months at 8 °C. The totipotency of the microencapsulated cells was proven. Cells that were not spray dried regenerated 24.6 plantlets, while the spray dried cells regenerated 19 plantlets for each gram of rehydrated powder. Thus, spray drying allowed researchers to obtain viable and totipotent cells. This work is the first manuscript that reported the spray drying of plant somatic cells.José Alfredo Santiz-GómezMiguel Abud-ArchilaVíctor Manuel Ruíz-ValdiviezoYazmin Sánchez-RoqueFederico Antonio Gutiérrez-MiceliMDPI AGarticlecarrotencapsulationsomatic cellsspray dryingBotanyQK1-989ENPlants, Vol 10, Iss 2491, p 2491 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	carrot encapsulation somatic cells spray drying Botany QK1-989
spellingShingle	carrot encapsulation somatic cells spray drying Botany QK1-989 José Alfredo Santiz-Gómez Miguel Abud-Archila Víctor Manuel Ruíz-Valdiviezo Yazmin Sánchez-Roque Federico Antonio Gutiérrez-Miceli Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
description	The carrot is considered a model system in plant cell culture. Spray drying represents a widely used technology to preserve microorganisms, such as bacteria and yeasts. In germplasm conservation, the most used methods are freeze drying and cryopreservation. Therefore, the aim of this work was to evaluate the effect of spray drying on the viability and totipotency of somatic carrot cells. Leaf, root and stem explants were evaluated to induce callus with 2 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). Calli obtained from the stem were cultivated in a liquid medium with 1 mg/L of 2,4-D. Cell suspensions were spray dried with maltodextrin-gum Arabic and maltodextrin-xanthan gum mixtures, two outlet air temperatures (50 and 60 °C) and 120 °C inlet air temperature. Results showed that carrot cells were viable after spray drying, and this viability remained for six months at 8 °C. The totipotency of the microencapsulated cells was proven. Cells that were not spray dried regenerated 24.6 plantlets, while the spray dried cells regenerated 19 plantlets for each gram of rehydrated powder. Thus, spray drying allowed researchers to obtain viable and totipotent cells. This work is the first manuscript that reported the spray drying of plant somatic cells.
format	article
author	José Alfredo Santiz-Gómez Miguel Abud-Archila Víctor Manuel Ruíz-Valdiviezo Yazmin Sánchez-Roque Federico Antonio Gutiérrez-Miceli
author_facet	José Alfredo Santiz-Gómez Miguel Abud-Archila Víctor Manuel Ruíz-Valdiviezo Yazmin Sánchez-Roque Federico Antonio Gutiérrez-Miceli
author_sort	José Alfredo Santiz-Gómez
title	Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
title_short	Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
title_full	Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
title_fullStr	Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
title_full_unstemmed	Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology
title_sort	totipotency of <i>daucus carota</i> l. somatic cells microencapsulated using spray drying technology
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/00dfbc47924e4efd8e8bdccc1c377b8a
work_keys_str_mv	AT josealfredosantizgomez totipotencyofidaucuscarotailsomaticcellsmicroencapsulatedusingspraydryingtechnology AT miguelabudarchila totipotencyofidaucuscarotailsomaticcellsmicroencapsulatedusingspraydryingtechnology AT victormanuelruizvaldiviezo totipotencyofidaucuscarotailsomaticcellsmicroencapsulatedusingspraydryingtechnology AT yazminsanchezroque totipotencyofidaucuscarotailsomaticcellsmicroencapsulatedusingspraydryingtechnology AT federicoantoniogutierrezmiceli totipotencyofidaucuscarotailsomaticcellsmicroencapsulatedusingspraydryingtechnology
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Totipotency of <i>Daucus carota</i> L. Somatic Cells Microencapsulated Using Spray Drying Technology

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