Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests

Entomopathogenic fungi are widely used as biocontrol agents against several agricultural pests. Among them, Beauveria bassiana is considered the important one against insect and other arthropod pests. The entomotoxic proteins of B. bassiana were extracted by Sephadex G-25 column, and fractionated us...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Majesh Tomson, Kitherian Sahayaraj, Samy Sayed, Sayed-Ashraf Elarnaouty, Ganeshan Petchidurai
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Beauveria bassiana Entomotoxin Characterization Insecticidal activity Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/00ec5cd70fb641868e75535752dcc4c1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:00ec5cd70fb641868e75535752dcc4c1
record_format	dspace
spelling	oai:doaj.org-article:00ec5cd70fb641868e75535752dcc4c12021-11-18T04:43:52ZEntomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests1018-364710.1016/j.jksus.2021.101595https://doaj.org/article/00ec5cd70fb641868e75535752dcc4c12021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1018364721002573https://doaj.org/toc/1018-3647Entomopathogenic fungi are widely used as biocontrol agents against several agricultural pests. Among them, Beauveria bassiana is considered the important one against insect and other arthropod pests. The entomotoxic proteins of B. bassiana were extracted by Sephadex G-25 column, and fractionated using HPLC (BBI, BBII and BBIII) and tested against two hemipteran insect pests i.e., Dysdercus cingulatus Fab. and Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae). Results indicated that protein content was higher in fraction BBII than BBI and BBIII. The vibration frequency in FT-IR obtained with a range of 1650 to 1580 cm−1. Bioassays of fractions (I, II and III) reveal that BBII was highly virulent against third nymphal instar of D. cingulatus (LC50 = 800.2 ppm) and adults of P. solenopsis adult (LC50 = 713.3 ppm). Considering the high virulence of BBII subjected to SDS-PAGE, HPLC and MALDI-TOF analyses. Analyses reveals the presence of 174 kDa and designated as BBF2. These results concluded that the entomotoxic protein of B. bassiana can be utilized for management of these investigated hemipreran pests. Further investigations are necessary for the field application of this entomotoxin against these pests or other insect pests. These results also could be helpful for establishing novel biotechnological uses for this fungus.Majesh TomsonKitherian SahayarajSamy SayedSayed-Ashraf ElarnaoutyGaneshan PetchiduraiElsevierarticleBeauveria bassianaEntomotoxinCharacterizationInsecticidal activityScience (General)Q1-390ENJournal of King Saud University: Science, Vol 33, Iss 8, Pp 101595- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Beauveria bassiana Entomotoxin Characterization Insecticidal activity Science (General) Q1-390
spellingShingle	Beauveria bassiana Entomotoxin Characterization Insecticidal activity Science (General) Q1-390 Majesh Tomson Kitherian Sahayaraj Samy Sayed Sayed-Ashraf Elarnaouty Ganeshan Petchidurai Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
description	Entomopathogenic fungi are widely used as biocontrol agents against several agricultural pests. Among them, Beauveria bassiana is considered the important one against insect and other arthropod pests. The entomotoxic proteins of B. bassiana were extracted by Sephadex G-25 column, and fractionated using HPLC (BBI, BBII and BBIII) and tested against two hemipteran insect pests i.e., Dysdercus cingulatus Fab. and Phenacoccus solenopsis Tinsely (Hemiptera: Pseudococcidae). Results indicated that protein content was higher in fraction BBII than BBI and BBIII. The vibration frequency in FT-IR obtained with a range of 1650 to 1580 cm−1. Bioassays of fractions (I, II and III) reveal that BBII was highly virulent against third nymphal instar of D. cingulatus (LC50 = 800.2 ppm) and adults of P. solenopsis adult (LC50 = 713.3 ppm). Considering the high virulence of BBII subjected to SDS-PAGE, HPLC and MALDI-TOF analyses. Analyses reveals the presence of 174 kDa and designated as BBF2. These results concluded that the entomotoxic protein of B. bassiana can be utilized for management of these investigated hemipreran pests. Further investigations are necessary for the field application of this entomotoxin against these pests or other insect pests. These results also could be helpful for establishing novel biotechnological uses for this fungus.
format	article
author	Majesh Tomson Kitherian Sahayaraj Samy Sayed Sayed-Ashraf Elarnaouty Ganeshan Petchidurai
author_facet	Majesh Tomson Kitherian Sahayaraj Samy Sayed Sayed-Ashraf Elarnaouty Ganeshan Petchidurai
author_sort	Majesh Tomson
title	Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
title_short	Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
title_full	Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
title_fullStr	Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
title_full_unstemmed	Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests
title_sort	entomotoxic proteins of beauveria bassiana bals. (vuil.) and their virulence against two cotton insect pests
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/00ec5cd70fb641868e75535752dcc4c1
work_keys_str_mv	AT majeshtomson entomotoxicproteinsofbeauveriabassianabalsvuilandtheirvirulenceagainsttwocottoninsectpests AT kitheriansahayaraj entomotoxicproteinsofbeauveriabassianabalsvuilandtheirvirulenceagainsttwocottoninsectpests AT samysayed entomotoxicproteinsofbeauveriabassianabalsvuilandtheirvirulenceagainsttwocottoninsectpests AT sayedashrafelarnaouty entomotoxicproteinsofbeauveriabassianabalsvuilandtheirvirulenceagainsttwocottoninsectpests AT ganeshanpetchidurai entomotoxicproteinsofbeauveriabassianabalsvuilandtheirvirulenceagainsttwocottoninsectpests
_version_	1718425078847242240

Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests

Ejemplares similares