Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres

Abstract Apoptosis is a physiological process that occurs commonly during the development of the preimplantation embryo. The present work examines the ability of apoptotic embryonic cells to express a signal promoting their phagocytosis, and quantifies the ability of neighbouring, normal embryonic c...

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Autores principales:	Jozef Pisko, Alexandra Špirková, Štefan Čikoš, Lucia Olexiková, Veronika Kovaříková, Zuzana Šefčíková, Dušan Fabian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00f6b50ec69b4f2aacf1d911ccd6acab
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spelling	oai:doaj.org-article:00f6b50ec69b4f2aacf1d911ccd6acab2021-12-02T13:41:43ZApoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres10.1038/s41598-021-88752-02045-2322https://doaj.org/article/00f6b50ec69b4f2aacf1d911ccd6acab2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88752-0https://doaj.org/toc/2045-2322Abstract Apoptosis is a physiological process that occurs commonly during the development of the preimplantation embryo. The present work examines the ability of apoptotic embryonic cells to express a signal promoting their phagocytosis, and quantifies the ability of neighbouring, normal embryonic cells to perform that task. Microscopic analysis of mouse blastocysts revealed phosphatidylserine externalization to be 10 times less common than incidence of apoptotic cells (as detected by TUNEL). In spite of the low frequency of phosphatidylserine-flipping (in inner cell mass, no annexin V staining was recorded), fluorescence staining of the plasma membrane showed more than 20% of apoptotic cells to have been engulfed by neighbouring blastomeres. The mean frequency of apoptotic cells escaping phagocytosis by their extrusion into blastocyst cavities did not exceed 10%. Immunochemically visualised RAC1 (an enzyme important in actin cytoskeleton rearrangement) was seen in phagosome-like structures containing a nucleus with a condensed morphology. Gene transcript analysis showed that the embryonic cells expressed 12 receptors likely involved in phagocytic process (Scarf1, Msr1, Cd36, Itgav, Itgb3, Cd14, Scarb1, Cd44, Stab1, Adgrb1, Cd300lf, Cd93). In conclusion, embryonic cells possess all the necessary mechanisms for recognising, engulfing and digesting apoptotic cells, ensuring the clearance of most dying blastomeres.Jozef PiskoAlexandra ŠpirkováŠtefan ČikošLucia OlexikováVeronika KovaříkováZuzana ŠefčíkováDušan FabianNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jozef Pisko Alexandra Špirková Štefan Čikoš Lucia Olexiková Veronika Kovaříková Zuzana Šefčíková Dušan Fabian Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
description	Abstract Apoptosis is a physiological process that occurs commonly during the development of the preimplantation embryo. The present work examines the ability of apoptotic embryonic cells to express a signal promoting their phagocytosis, and quantifies the ability of neighbouring, normal embryonic cells to perform that task. Microscopic analysis of mouse blastocysts revealed phosphatidylserine externalization to be 10 times less common than incidence of apoptotic cells (as detected by TUNEL). In spite of the low frequency of phosphatidylserine-flipping (in inner cell mass, no annexin V staining was recorded), fluorescence staining of the plasma membrane showed more than 20% of apoptotic cells to have been engulfed by neighbouring blastomeres. The mean frequency of apoptotic cells escaping phagocytosis by their extrusion into blastocyst cavities did not exceed 10%. Immunochemically visualised RAC1 (an enzyme important in actin cytoskeleton rearrangement) was seen in phagosome-like structures containing a nucleus with a condensed morphology. Gene transcript analysis showed that the embryonic cells expressed 12 receptors likely involved in phagocytic process (Scarf1, Msr1, Cd36, Itgav, Itgb3, Cd14, Scarb1, Cd44, Stab1, Adgrb1, Cd300lf, Cd93). In conclusion, embryonic cells possess all the necessary mechanisms for recognising, engulfing and digesting apoptotic cells, ensuring the clearance of most dying blastomeres.
format	article
author	Jozef Pisko Alexandra Špirková Štefan Čikoš Lucia Olexiková Veronika Kovaříková Zuzana Šefčíková Dušan Fabian
author_facet	Jozef Pisko Alexandra Špirková Štefan Čikoš Lucia Olexiková Veronika Kovaříková Zuzana Šefčíková Dušan Fabian
author_sort	Jozef Pisko
title	Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
title_short	Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
title_full	Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
title_fullStr	Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
title_full_unstemmed	Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
title_sort	apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/00f6b50ec69b4f2aacf1d911ccd6acab
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Apoptotic cells in mouse blastocysts are eliminated by neighbouring blastomeres

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