Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone

Abstract The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jinho Bang, Heesun Park, Jihye Yoo, Donghyun Lee, Won Il Choi, Jin Hyung Lee, Young-Ran Lee, Chungho Kim, Heebeom Koo, Sunghyun Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/00f7dbf6beec41eaa002d16d4917a8d2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:00f7dbf6beec41eaa002d16d4917a8d2
record_format	dspace
spelling	oai:doaj.org-article:00f7dbf6beec41eaa002d16d4917a8d22021-12-02T16:31:53ZSelection and identification of a novel bone-targeting peptide for biomedical imaging of bone10.1038/s41598-020-67522-42045-2322https://doaj.org/article/00f7dbf6beec41eaa002d16d4917a8d22020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-67522-4https://doaj.org/toc/2045-2322Abstract The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (~ 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications.Jinho BangHeesun ParkJihye YooDonghyun LeeWon Il ChoiJin Hyung LeeYoung-Ran LeeChungho KimHeebeom KooSunghyun KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinho Bang Heesun Park Jihye Yoo Donghyun Lee Won Il Choi Jin Hyung Lee Young-Ran Lee Chungho Kim Heebeom Koo Sunghyun Kim Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
description	Abstract The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (~ 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications.
format	article
author	Jinho Bang Heesun Park Jihye Yoo Donghyun Lee Won Il Choi Jin Hyung Lee Young-Ran Lee Chungho Kim Heebeom Koo Sunghyun Kim
author_facet	Jinho Bang Heesun Park Jihye Yoo Donghyun Lee Won Il Choi Jin Hyung Lee Young-Ran Lee Chungho Kim Heebeom Koo Sunghyun Kim
author_sort	Jinho Bang
title	Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
title_short	Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
title_full	Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
title_fullStr	Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
title_full_unstemmed	Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
title_sort	selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/00f7dbf6beec41eaa002d16d4917a8d2
work_keys_str_mv	AT jinhobang selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT heesunpark selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT jihyeyoo selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT donghyunlee selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT wonilchoi selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT jinhyunglee selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT youngranlee selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT chunghokim selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT heebeomkoo selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone AT sunghyunkim selectionandidentificationofanovelbonetargetingpeptideforbiomedicalimagingofbone
_version_	1718383791468183552

Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone

Ejemplares similares